Utilizing trypsin enzyme in the diet and their effects on growth, body composition, some blood biochemical factors and activity of intestinal trypsin of great sturgeon (Huso huso)

Document Type : Nutrition

Authors

1 Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, PO Box: 487-49175

2 Institute of Fisheries Research, Agricultural Research, Education and Promotion Organization, Tehran, PO Box: 6116-14155

3 Department of Aquaculture Processing, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, PO Box: 487-49175

4 Department of Materials Science and Design, Food Science and Technology Faculty, Gorgan University of Agricultural Sciences and Natural Resources, PO Box: 487-49175

Abstract

Exogenous proteolytic enzyme supplementations can eliminate the effects of antinutritional factors and to improve fish growth performance. The purpose of this study was to investigate the utilizing porcine trypsin in the diet and its effects on growth factors, body composition, some blood biochemical parameters and activity of intestinal trypsin in great sturgeon (Huso huso). The experiment was conducted in completely randomized design with 3 treatments and 3 replications and trypsin at 3 levels of 0, 0.01% and 0.02% was added to the diet and given the experimental diets to fishes for 45 days and growth factors, Weight growth percent (WGP), specific growth rate (SGR), feed conversion ratio (FCR), Condition factor (CF), Protein efficiency ratio (PER), Hepato-somatic index were studied. Blood biochemical factors glucose, BUN, total protein, albumin, globulin and A: G ratio were measured and evaluated. Different treatments of (CF) were not significantly different from each other (P> 0.05), But (WGP), (SGR), (FCR) and (PER), the enzyme levels of 0.01% and 0.02 were significantly different from control group (P <0.05). HSI and activity of intestinal trypsin in enzyme levels of 0.02% was significantly different from control group (P <0.05). Body composition and blood biochemical parameters were not significantly different between enzyme levels (P> 0.05).

Keywords

References

  1.  

    1. AOAC (Association of Official Analytical Chemists), 2005. Official Methods of Analysis 16th edition. AOAC, Gaithersburg, Maryland. 532 P.
    2. Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal. Biochem. Vol. 72,
      pp: 248-254.
    3. Carter, C.G.; Houlihan, D.F.; Buchanan, B. and Mitchell, A.I., 1994. Growth and feed utilization efficiencies of seawater Atlantic salmon Salmo salar L. fed a diet containing supplementary enzymes. Aquaculture research. Vol. 25,
      No. 1, pp: 37-46.
    4. Chen, J.M.; Ye, J.Y.; Xu, Y.X.; Shen, B.Q.; Guo, J.L.; Pan, Q. and Wang, Y.H., 2009. Effect of adding neutral protease to diets on growth performance, digestion, and body composition of fingerling black carp (Mylopharyngodon piceus). Acta Hydrobiologica Sinica. Vol. 33, No. 4,
      pp: 726-731.
    5. Dabrowska, H.; Grudniewski, H. and Dabrowski,
      K., 1979.       Artificial diets for common carp: effect of the addition of enzyme extracts. The Progressive Fish-Culturist. Vol. 41, No. 4, pp: 196-200.
    6. Dabrowski, K. and Glogowski, J., 1977. A study of the application of proteolytic enzymes to fish food. Aquaculture. Vol. 12, pp: 349-360.
    7. Dalsgaard, J.; Verlhac, V.; Hjermitslev, N.; Ekmann, K.S.; Fischer, M.; Klausen, M. and Pedersen, P.B., 2012. Effects of exogenous enzymes on apparent nutrient digestibility in rainbow trout (Oncorhynchus mykiss) fed diets with high inclusion of plant-based protein. Animal feed science and technology. Vol. 171, pp: 181-91.
    8. Divakaran, S. and Velasco, M., 1999. Effect of proteolytic enzyme addition to a practical feed on growth of the Pacific white shrimp, Litopenaeus vannamei (Boone). Aquaculture Research. Vol. 30, pp: 335-339.
    9. Erlanger, B.F.; Kokowski, N. and Cohen, W., 1961. The preparation and properties of two new chromogenic substrates of trypsin. Archive Biochemistry and Biophysics. Vol. 95, pp: 271-278.
    10. Farhangi, M. and Carter, C.G., 2007. Effect of enzyme supplementation to dehulled lupin‐based diets on growth, feed efficiency, nutrient digestibility and carcass composition of rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research. Vol. 38, pp: 1274-1282.
    11. Furne, M.; García-Gallego, M.; Hidalgo, M.C.; Morales, A.E.; Domezain, A.; Domezain,J. and Sanz, A., 2008. Effect of starvation and refeeding on digestive enzyme activities in sturgeon (Acipenser naccarii) and trout (Oncorhynchus mykiss). Comp. Biochem. Physiol. Vol. 149, pp: 420-425.
    12. Ghomi, M.R.; Shahriari, R.; Langroudi, H.F.; Nikoo, M. and von Elert, E., 2012. Effects of exogenous dietary enzyme on growth, body composition, and fatty acid profiles of cultured great sturgeon Huso huso fingerlings. Aquaculture International. Vol. 20, pp: 249-254.
    13. Kolkovski, S., Tandler, A. and Izquierdo, M., 1997. Effects of live food and dietary digestive enzymes on the efficiency of microdiets for seabass (Dicentrarchus labrax) larvae. Aquaculture. Vol. 148, pp: 313-322.
    14. Kumari, R.; Gupta, S.; Singh, A.R.; Ferosekhan, S.; Kothari, D.C.; Kumar, P.A. and Jadhao, S.B., 2013. Chitosan nanoencapsulated exogenous trypsin biomimics zymogen-Like enzyme in fish gastrointestinal tract. Plos One. Vol. 8, No. 9, pp: 1-12.
    15. Leng, X.; Liu, D.; Li, X. and Lu, Y., 2008. Effects of adding Protease AG on growth and digestive protease activities of common carp (Cyprinus carpio) fingerling. Chinese Journal of Animal Nutrition. Vol. 20, No. 3, pp: 1-7.
    16. Li, X.; Chai, X.; Liu, D.; Chowdhury, K. and Leng,
      X., 2015.       Effects of temperature and feed processing on protease activity and dietary protease on growths of white shrimp, Litopenaeus vannamei, and tilapia, Oreochromis niloticus× O. aureus. Aquaculture Nutrition. Vol. 21, No. 4, pp: 1-10.
    17. Lin, S.; Mai, K. and Tan, B., 2007. Effects of exogenous enzyme supplementation in diets on growth and feed utilization in tilapia, Oreochromis niloticus x O. aureus. Aquaculture Research. Vol. 38, pp: 1645-1653.
    18. Ogunkoya, A.E.; Page, G.I.; Adewolu, M.A. and Bureau, D.P., 2006. Dietary incorporation of soybean meal and exogenous enzyme cocktail can affect physical characteristics of faecal material egested by rainbow trout (Oncorhynchus mykiss). Aquaculture. Vol. 254 pp: 466-475.
    19. Patil, D. and Singh, H., 2013. Effect of papain supplemented diet on growth and survival of post-larvae of Macrobrachium rosenbergii. International Journal of Fisheries and Aquatic Studies. Vol. 1, No. 6, pp: 176-179.
    20. Ross, L.G. and Ross, B., 2008. Anaesthesia and Sedation of Aquatic Animals. 3rd ed. Oxford, Blackwell Science. 236 P.
    21. Shi, Z.; Li, X.Q.; Chowdhury, M.K.; Chen, J.N. and Leng, X.J., 2016. Effects of protease supplementation in low fish meal pelleted and extruded diets on growth, nutrient retention and digestibility of gibel carp, Carassius auratus gibelio. Aquaculture. Vol. 460, pp: 37-44.
    22. Singh, P.; Maqsood, S.; Samoon, M.H.; Phulia, V.; Danish, M. and Singh-Chalal, R., 2011. Exogenous supplementation of papain as growth promoter in diet of fingerlings of Cyprinus carpio. International Aquatic Research. Vol. 3, pp: 1-9.
    23. Song, H.L.; Tan, B.P.; Chi, S.Y.; Liu, Y.; Chowdhury, M. and Dong, X.H., 2016. The effects of a dietary protease‐complex on performance, digestive and immune enzyme activity, and disease resistance of Litopenaeus vannamei fed high plant protein diets. Aquaculture Research. Vol. 47,
      No. 5, pp: 1-11.
    24. Subasinghe, R.P., 2005. Epidemiological approach to aquatic animal health management: opportunities and challenges for developing countries to increase aquatic production through aquaculture. Preventive Veterinary Medicine. Vol. 67, pp: 117-124.
    25. Tacon, A.G.J., 1990. Standard methods for the nutrition and feeding of farmed fish and shrimp. Washington DC, Argent Laboratories press. 454 P.
    26. Vielma, J.; Makinen, T.; Ekholm, P. and Koskela,
      J., 2000.       Influenceof dietary soy and phytase levels on performance and body composition of large rainbow trout (Oncorhynchus mykiss) andalgal availability of phosphorus load. Aquaculture. Vol. 183, pp: 349-362.
    27. Zamini, A.A.; Kanani, H.G.; Esmaeili, A.A.; Ramezani, S. and Zoriezahra, S.J., 2014. Effects of two dietary exogenous multi-enzyme supplementation, Natuzyme® and beta-mannanase (Hemicell®), on growth and blood parameters of Caspian salmon (Salmo trutta caspius). Comparative Clinical Pathology. Vol. 23, pp: 187-192.

 

Journal of Animal Environment
Volume 9, Issue 3
September 2017
Pages 211-218

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