A comparison investigation in serum protein analytical and electrophoretical pattern between Arab horses and Caspian ponies(Equus ferus caballus)

Document Type : Physiology (Animal)

Author

Department of Cellular and Molecular Biology, Lahijan Unit, Islamic Azad University, Lahijan, Iran

Abstract

Caspin pony & Persian Arab horses were an endemic and proper strain in Persia for thousand years.Proteins apply a grate roll as enzymes, hormones, antibodies and coagollation agents etc.This investigation were done on 86 Persian horses  and 94 Caspian ponies blood serums.Total protein using Biurett reaction estimated by Elan automated analyzer and serum proteins fractionation and separation analyzed  by Sebia K20 agarose gel electrophoresis system techniques.Of Persian arab horses total protein 7.2±0.64,albumin2.9±0.56,α1-glubolins fraction 0.2±0.09,α2-glubolins fraction 1.2±0.28,β glubolins0.1±0.18,γ1-glubolins fraction 0.7±0.85,γ2-glubolins fraction 1.2±0.4 and mean total glubolin were 4.2±0.55 g/dl and alb/glu ratio was 0.7±0.18 in contrast in Caspian ponies total protein 7.34±0.8, albumin3.68±0.03,α1-glubolins fraction 0.19±0.004,α2-glubolins fraction 0.78±0.01,β1-glubolin fraction 0.71±0.04, β2-glubolins fraction 0.46±0.01, γ glubolins1.54±0.02 and mean total glubolin were 3.65±0.05 g/dl whereas alb/glu ratio determined by 1.01±0.01.Our findings  showed  drastic differences in comparison to Christense (1977) and Rashidinia(1995) but more confidences with and Osbaldiston(1972) reported results noting to α2, β1- and β2-glubolins fractions laid in albumin and γ glubolin zones.

Keywords

References

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Journal of Animal Environment
Volume 9, Issue 4
January 2018
Pages 41-48

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