Molecular Phylogeny of Vetigastropoda Based on Mitochondrial Genes, COI and 16S rRNA

Document Type : Animal systematic

Authors

1 Research group of Biodiversity & Biosafety, Research Center for Environment and Sustainable Development, RCESD, Department of Environment, Tehran, Islamic Republic of Iran

2 Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology

3 Department of Marine Biology, Faculty of Marine Science and Technology, Islamic Azad University North Tehran Branch

4 Department of Biology, Faculty of Biolobical Science, Islamic Azad University Varamin Pishva Branch

Abstract

The molecular phylogeny of Vetigastropoda species have been studied based on mitochondrial genes, COI and 16S rRNA in the present work for the first time in the northern rocky coastal zones of Persian Gulf during years 2013 and 2014. After morphological identifications of species, DNA extraction, amplifying partial of cytochrome oxidase COI and 16S rRNA and sequencing procedure were done in the laboratory. In this study 9 COI and 5 16S rRNA sequencing belonging to 5 species were measured. Also, phylogeny analyses with drawing phylogeny trees of Maximum Likelihood, Maximum Parsimony and Bayesian were done using MEGA6, PAUP and BEAST softwares. The result showed that clade Vetigastropoda, include Trochidae, Chilodontidae, Turbinidae and Fissurellidae families, is not monophyletic, which is not as classic identification. So Trochidae and Chilodontidae are in seprated clade from Turbinidae and Fissurellidae clade᾽s. This showed not monophyletic relation between this two group which represented difference between classic and molecular classification.

Keywords

References

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Journal of Animal Environment
Volume 10, Issue 2
July 2018
Pages 251-260

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