The chemical structure of acid-soluble Collagen from the Skin of Rutilus Kutum of Caspian Sea and Scomberomorus guttatus of Persian Gulf

Document Type : Animal science

Authors

1 Department of Marine Biology, Science and Research Branch, Islamic Azad university, Tehran, iran.

2 Agricultural Research and Development Organization, Fisheries Research Institute of Iran, Tehran, Iran

3 Plastic & amp; Reconstructive Surgery Department, Burn Research Center, Tehran, Iran.

4 pharmacology Department , Iran University of Medical Sciences, Tehran, Iran

5 Biotechnology Department , Baqiyatallah University of Medical Sciences

Abstract

Collagen has a wide range of applications in biomedical, pharmaceutical, cosmetic, and food industries. Collagen from cow has a risk for contamination from bovine spongiform encephalopathy (BSE), transmissible spongiform encephalopathy (TSE). This study proceeds to extract collagen from the skin of  Rutilus Kutum main and economical species of the Caspian Sea and Scomberomorus guttatus. Scomberomorus guttatus  one of the tuna species of Persian Golf. Acid-soluble collagens (ASC) from the fish skin of Scomberomorus guttatus and Rutilus Kutum were extracted and characterized by Pico.Tag, SDS-PAGE, FTIR and UV spectrophotometry. Based on the data from the SDS-PAGE and the composition of amino acid, both collagens  were probable that the obtained collagen classified as type I collagen. Collagens consisted of two different types of α chains, including α1 and  α2,and  β component. Also, FTIR analysis revealed the presence of helical arrangements of collagens. Moreover, UV spectrophotometry exhibited this collagen have an absorbance at 220 nm. According to the basis of dry weight, the yield of Rutilus Kutum  and Scomberomorus guttatus ASC were calculated 15.6 % and 14.5 %. Amino acid analysis revealed a much higher glaycin content in the both collagens extracted which accounted to one third of the total amino acids. Besides, proline as an unique amino acid in Rutilus Kutum  and Scomberomorus guttatus ASC had the specific amount of89.6  and  86.8 residues per 706.1 residues.

Keywords

References

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Journal of Animal Environment
Volume 10, Issue 3
October 2018
Pages 241-250

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