Evaluating the possible causes of wild common carp (Cypirnus carpio) mortality cultivated in pen in Gorgan Bay

Document Type : Disease

Authors

1 Inland Aquatic Reserves Research Center, National Institute of Fisheries Research, Agricultural Research, Education and Extension Organization, Gorgan, Iran

2 Fisheries department, animal science and fisheries faculty, sari agricultural sciences and natural resources university, Sari, Iran

Abstract

In this research investigated the possible causes of mortality of cultivated wild Carp containing 2 densities (2 and 4 individual/m2) in pens in Gorgan Bay between May and June 2013. Mortality started with increasing the temperature (29 till 30.8 °c) in July. Clinical symptoms observed in sick fish include fever, swimming in surface, dark color, Ventricular dilatation, one and two sides exophthalmia and hemorrhage on the  body surface. 50 samples of fish at the time of death for autopsy were transferred to the laboratory. The internal organs (kidney and liver) were cultured in the TSA medium. After incubation, purified colonies, the bacteria were identified using diagnostic tests. The results proved that the isolated bacteria were Aeromonas hydrophila.The result of antibiogram test was performed the sensitivity of bacteria to Antroflexacin. To compare mortality in two treatments, it used of the non-parametric tests of Man-Witney and to compare the different times in each of the densities used of Kruskal-Walis test. The results showed there was no significant differences between two treatments (2 and 4 individual/m2) in each months.

Keywords

References

  1. Adanir, D.O.R. and Turutoglu, H., 2007. Isolation and antibiotic susceptibility of Aeromonas hydrophila in a carp (Cyprinus carpio) hatchery farm. Bulletin of theVeterinary Institute in Pulawy. Vol. 51, pp: 361-364.
  2. Austin, B. and Austin, D.A., 2007. Bacterial Fish Pathogenes,4th ed. Chichester, UK: Springer- Praxis. 81 p.
  3. Buller, N.B., 2004. Bacteria from fish and other aquatic animals: a practical identification manual. CABI
    publishing. 361 p.
  4. Halmer, J.E., 1988. Fish nutrition. 2nd ed. Sandieo Academic press. 384 p.
  5. Jester, D.B., 1974. Life history, ecology and management of the carp Cyprinus carpio Linnaeus .in Elephant Butte Lake.New Mexico State Univ. Ag. Exp. sta. Res. Rep. pp: 80-273.
  6. Lee, S.; Kim, S.; Oh, Y.  and Lee, Y., 2002. Characterization of Aeromonas hydrophila, isolated from rainbow trouts in Korea. The Journal of Microbiology. Vol. 38, No. 1, pp: 1-7.
  7. Holt, J. and Krieng, N., 1994. Bergey’s Manual of Determinative Bacteriology. 9th Edition, The Williams
    comp. 787 p.
  8. Nielsen, M.E.; Høi, L.; Schmidt, A.S.; Qian, D.; Shimada, T.; Shen, J.Y. and Larsen, J.L., 2001. Is Aeromonas hydrophila the dominant motile aeromonas species that causes disease outbreaks in aquaculture production in the Zhejiang province of china? Disease of Aquatic Organisms. Vol. 46, No. 22, pp: 23-29.
  9. NRC. 2011. Nutrient Requirement of Fish and Shrimp. Committee on Animal Nutrition, Board 32, on Agriculture, National Research Council, National Academy press, Washington, D.C., USA. 392 p.
  10. Porteen, K.; Agarwal, R.K. and Bhilegaonkar, K.N., 2006. PCR Based Detection of Aerominas from Milk Samplees.  Journal of Food Technology. Vol. 4, No. 2, pp: 111-115.
  11. Rey, A.N.; Verján, H.W.; Ferguson, C. and Iregui, I., 2009. Pathogenesis of Aeromonas hydrophila strain KJ99 infection and its extracellular products in two species of fish. Veterinary Record. Vol. 164, pp: 493-499.
Journal of Animal Environment
Volume 11, Issue 1
May 2019
Pages 217-224

Files

Share

How to cite

Statistics