Effect of orally administration of Lactoferrin, β-glucan and Nigella sativa on the growth indexes and hematological and immunological parameters in common carp Cyprinus carpio

Document Type : Nutrition

Authors

Department of Fisheries, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Iran, PO Box: 163

Abstract

In order to compare the herbal and chemical immunity stimulants, the effect of orally administration of Lactoferrin, β-glucan and Nigela sativa on growth indexes and hematological and immunological parameters in common carp was studied. 300 fingerling (3±0.02 g), were fed with control food (without immune stimulant) and food treatments include 20 mg lactoferrin, 10 mg  β-glucan and 50 mg Nigela sativa powder in kg of food for sixty days. Based on the results, the highest amounts of weight increase, specific growth rate and condition factor were obtained in the glucan treatment. The survival rate was above 80 percent, without significant difference, in all treatments (P>0.05). Also, all treatments include immune stimulant, showed significant difference in food conversion rate (FCR) compare to control group (P<0.05). The minimum amount of FCR and the maximum amount of protein coefficient were obtained in glucan treatment. The higher numbers of red and white blood cells and the higher amounts of hemoglobin and hematocrit were observed in glucan and the lower were observed in the control group. The higher and the lower rate of lysozyme enzyme activity were related to glucan (5.38± 0.18) and control treatments (3.19± 0.05) respectively. While the percentage of neutrophil and monocytes were higher in glucan treatment, the highest percentage of lymphocytes was observed in lactoferrin treatment (73.66± 0.57). So, compare to the other stimulants, glucan can be used as a growth and immunity stimulant in common carp.

Keywords


  1. Ai,Q.; Mai, K.; Zhang, L.; Tan, B.; Zhang, W. and Xu, W., 2007. Effects of dietary β-1, 3 glucan on innate immune response of large yellow croaker (Pseudosciaena crocea). Fish and Shellfish Immunology. Vol. 22, pp: 394-402.
  2. Andrews, S.R.; Sahu, N.P. and Pal, A., 2009. Growth of Labeo rohita fingerlings: Effect of dietary mannan oligosaccharide, yeast extract, protein hydolysate and chlorella. Aquaculture Research. Vol. 41, pp: 61-69.
  3. Ardo, L.; Yin, G.; Xu, P.; Varadi, L.; Szigeti, G. and Jeney, G., 2008.Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis nitloticus) and reistance against Aeromonas hydrophyla. Aquaculture. Vol. 275, pp: 26-33.
  4. Blaxhall, P.C. and Daisley, K., 1973. Routine haematological methods for use with fish bloods. Journal of Fish Biology. Vol. 5, pp: 771-781.
  5. Cook, M.T.; Hayball, P.J.; Hutchinson, W.; Nowak, B.F. and Hayball, J.D., 2003. Administration of a commercial immunostimulant preparation, Ecoactiva as a feed supplement enhances macrophase respiratory burst and the growth rate of snapper (Pagrus auratus), Sparidae (Bloch and Schneider) in winter. Fish Shellfish Immunology. Vol. 14, pp: 333-345.
  6. Eliss, A.E., 1990. Lysozyme assay. In: Stolen J.S., Fletcher, D.P., Anderson, B.S. and Robertson, B. S.(eds). Techniques in Fish immunology. Fair Haven, N J, USA SOS Publication. pp:101-103.
  7. Galina, J.; Yin, G.; Ardo, L. and Jeney, Z., 2009. The use of immunostimulating herbs in fish. An overview of research. Fish Physiology and Biochemistry. Vol. 35, No. 4, pp: 669-676.
  8. Houston, A.H.; Dobric, N. and Kahurananga, R., 1996. The nature of hematological response in fish. Studies on rainbow trout Oncorhynchus mykiss exposed to stimulated winter, spring and summer conditions. Fish Physiology and Biochemistry. Vol. 15, pp: 339-347.
  9. Irianto, A. and Austin, B., 2002. Probiotic in aquaculture. Fish Disease. Vol. 25, pp: 1-10.
  10. Jaramillo, J.F. and Gatlin, D.M., 2004. Comparison of purified and practical diets supplemented with or without β glucan and selenium on resistance of hybrid striped bass (Morone chrysops* M. saxatilis) to Streptococcus iniae infection. World Aquaculture Society. Vol. 35, pp: 245-252.
  11. Kakuta, I., 1996. Protective effect of orally administrated bovine lactoferrin against experimental infection of goldfish (Carassius auratus) with Ichthyophthirius multifiliis. Aquaculture Science. Vol. 44, pp: 427-432.
  12. Kim, S.K. and Rajapakse, N., 2005. Enzymeatic production and biological activities of chitosan oligosaccharides (COS): a review. Carbohydrate Research. Vol.62, pp: 357-368.
  13. Klontz, G.W., 1994. Fish hematology. In: Techiques in fish immunology. Stolen, J.S., Fletcher, T.C., Rowley, A.F., Kelikoff, T.C., Kaaraii. S.L. and Smith, S.A. (eds). Vol.3.SOS Publications. Fair Haven.New Jersey, USA. pp: 121-132.
  14. Kumari, J.; Sahoo, P.K.; Swain, T.; Sahoo, S.K.; Sahu, B. and Mohanty, B.R., 2006. Seasonal variation in the innate immune parameters of the Asia cat fish Clarias batrachus. Aquaculture. Vol. 252, pp: 121-127.
  15. Lin, S.; Pan, Y.; Luo, L. and Luo, L., 2011. Effects of dietary b-1, 3-glucan, chitosan or raffinose on the growth, innate immunity and resistance of koi (Cyprinus carpio koi). Fish and Shellfish Immunology. Vol. 31, pp: 788-794.
  16. Maqsood, S.; Samoon, M.H. and Singh, P., 2009. Immunomodulatory and Growth Promoting Effect of Dietary Levamisole in Cyprinus carpio fingerlings against the challenge of Aeromonas hydrophila. Turkish Journal of Fish and Aquatic Sciences. Vol. 9, pp: 111-112.
  17. Muller, A.; Raptis, J.; Rice, P.J.; Kalbfleisch, J.H.; Stout, R.D.; Ensley, H.E.; Browder, W. and Williams, D.L., 2000. The influence of glucanpolymer structure and solution conformation on binding to (1_3)-β-D-glucan receptors in a human monocyte- like cell line, Glycobiology. Vol. 10, No. 4, pp: 339-346.
  18. NRC (National Research Council, USA). 1993. Nutrient Requirements of fish. National Academy of sciences, Washington. 124 p.
  19. Raa J., 1996. The use of immunostimulatory substance in fish and shellfish farming. Fisheries Science. Vol. 4, No. 3, pp: 229-288.
  20. Rehulka, J., 2000. Influence of astaxanthin on growth rate, condition, and some blood indices of rainbow trout, Oncorhynchus mykiss. Aquaculture. Vol.190, pp: 27-47.
  21. Ren, T.; Koshio, S.; Ishikawa, M.; Yokoyama, S.; Micheal, F.R. and Uyan, Q., 2007. Influence of dietary vitamin C and bovine Lactoferin on blood chemistry and non specific immune responses of Japanese eel, Anguilla japonica. Aquaculture. Vol. 267, pp: 31-37.
  22. Reverter, M.; Bontemps, N.; Lecchini, D.; Banaigs, B. and Sasal, P., 2014. Use of plant extracts in fish aquaculture as an alternative to chemotherapy: Current status and future Perspectives. Aquaculture. Vol. 433, pp: 50-61.
  23. Shalaby, A.M; Khattab, Y.A. and Abdel Rahman, A.M., 2006. Effect of garlic (Allium sativum) and chloram phenicol on growth performance, physiological parameters and survival of Nile tilapia (Oreochromis niloticus). Journal of Venomous Animal Toxins Including Tropical Disease. Vol. 12, pp: 172-201.
  24. Tomita, M.; Wakabayashi, H.; Shin, K.; Yamauchi, K.; Yaeshima, T.  and Iwatsuki, K., 2008. Twenty-five years of research on bovine lactoferin applications. Biochimie. Vol. 10, pp: 1-6.
  25. Walker, T.; Lin, C.; Yildirim- Aksoy M.; Shelby, R. and Klesius, P.H., 2007. Immune response and resistance to stress and Edwardsiella ictaluri challenge in channel catfish, Ictalurus pimctatus, fed diets containing commercial whole-cell yeast or yeast   subcompanents. Journal of   the World Aquaculture Society. Vol. 38, No. 1, pp: 24-35.
  26. Yin, G.; Ardo, L.; Thompson, K.D.; Adams, A.; Jeney, Z. and Jeney, G., 2009. Chinese herbs (Astragalus radix) and (Ganoderma lucidum) enhance immune response of crap, Cyprinus carpio, and protection against Aeromonas hydrophila. Fish and Shellfish Immunology. Vol. 26,
    pp: 140-145.
  27. Yokoyama, S.; Koshio, S.; Takakura, N.; Oshida, K.; Ishikawa, M. and Gallardo-Cigarroa, F.J., 2006. Effect of dietary bovine Lactoferin on growth response, tolerance to air exposure and low salinity stress conditions in orange spotted grouper (Epineohelus coioides). Aquaculture. Vol. 255, pp: 507-513.
  28. Yokoyama, S.; Koshi, S.; Takakura, N.; Oshida, K.; Ishikawa, M. and Gallardo- Cigarroa, F.J., 2005. Dietary bovine Lactoferin enhances tolerance to high temperature stress in Japanese flounder Paralichthys olivaceus. Aquaculture. Vol. 249, pp: 367-373.
  29. Yoshida, T.; Kruger, R. and Inglis, V., 1995. Augmentation of non-specific protection in African catfish, Clariasgraiepinus (Bruchell), by the long-term oral administration of immune stimulants. Journal of Fish Diseases. Vol. 18, pp: 195-198.