Isolation, Cultivation and Characterization of Neural stem cells from Persian Sturgeon (Acipenser persicus) Brain

Document Type : Biology(Animal)

Authors

1 Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Gorgan University of Medical Sciences, P.O.Box: 619, Gorgan, Iran

Abstract

In  the  present  study,  we  tried  to  cultivate  neural  stem  cells  from  different  regions  of Persian  sturgeon  (Acipenser  persicus)  brain,  as  a  chondrostei  fish.  For  this,  telencephalon, mesencephalon  and  metencephalon  of  the  brain  were  separated,  cultivated  in  DMEM/F12 medium  supplemented  with  fetal  bovine  serum  (20%  FBS)  and  maintained  at  250C  with 5%CO2.  Medium  was  changed  every  3s  days  and  replaced  with  fresh  medium.  After  2-3 weeks, cells were confluent and passaged. Cells of metencephalon (cerebellum) culture from Persian  sturgeon  brain  (CPS)  are  still  alive  after  about  7  months  and  9  passages  and  their stocks are available in the laboratory. The cell cultures have been cryopreserved using liquid nitrogen. The  survival rate  of cells  was 70%  after thawing.  In immonocytochemistry  survey, cells  were  immonopositive  for  anti-nestin  antibody,  as  a  neural  stem  cell  marker.  The obtained  cultures  could  be  considered  as  a  constant  long  term  culture  because  of  their stability, and could be used in different studies. 

Keywords

Journal of Animal Environment
Volume 5, Issue 2
September 2012
Pages 113-120

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