Genetic diversity of Oriental sole (Euryglossa orientalis) using microsatellite marker in the Persian Gulf (Khuzestan and Bushehr)

Document Type : Genetic

Authors

1 Department of Marine Biology, Faculty of Marine Science, Marine Science and Technology University, Khorramshahr, Iran

2 Department of Genetics, Faculty of Science, Shahid Chamran University, Khuzestan, P.O. Box: 61357-83151, Ahvaz, Iran

Abstract

The  aim  of  this  study  was  to  analysis  the  population  genetic  structure  of Eurygloss orientalis based  on  microsatellite  markers.  For  these  purpose  58  samples  of Eurygloss orientalis from  two  regions  of  Persian  Gulf  (Khuzestan  and  Bushehr)  were  collected.  DNA was  extracted  and  using  6  pairs  of  microsatellite  primers.  Polymerase  chain  reaction  (PCR) was conducted out of 6 microsatellite primers and results shows that 2 loci were polymorphic. Analysis revealed that the average number of observed and effective alleles in Khuzestan and Bushehr  were  14.5,  10.708  and  13.5,  9.511  respectively.  The  mean  observed  and  expected heterozygosity  was  0.837  and  0.889  respectively.  There  are  no  significant  deviations  from HWE.  The  Fst value  between  populations  was  0.025.  The  highest  genetic  distance  between Khuzestan  and  Bushehr  was  0.13  and  the  lowest  genetic  similarity  was  0.733.  The  Fst value suggested no genetic differences between populations.

Keywords

Journal of Animal Environment
Volume 5, Issue 3
November 2012
Pages 17-23

Files

Share

How to cite

Statistics