Sequence analysis of VP28 gene of white spot virus isolated from White Leg shrimp( Litopenaeus vannamei) in Khuzestan province-Iran

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran. Po. Box:61355-145

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran. Po. Box:61355-145

3 Iranian Fisheries Reseach Organization, South of Iran Aquaculture Reseach Center, Ahvaz,Iran. PO. Box:61645/866

4 Iranian Fisheries Reseach Organization, Tehran,Iran. PO. Box:14965/149.

Abstract

White spot disease is one of the most serious viral diseases which affect farmed shrimp in the world. In Iran the disease maked serious damages to shrimp farmers. During a culture period, shrimp farms of Abadan were visited and those shrimps with clinical symptoms of WSSD were sampled. The samples which had the highest frequency of white spot disease were used as a template in subsequent experiments. This isolate was named: IRI-KHZ/904. By designing of primers, VP28 gene amplification and sequence was recorded in gene bank with accession number: AB855742. The nucleotide sequences obtained in this study had 100% homology with most isolates from different countries which recorded in the gene bank. Also in comparison with 4 other isolates that had been recorded in gene bank, Iranian isolate showed 99% homology.

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