Investigation of two phenotypes of the Gorgani Cave Salamander (Paradactylodon gorganensis) Using D-loop Region Sequencing

Document Type : Genetic

Authors

1 Department of Biology, Faculty of Biological Sciences,Varamin-Pishva Branch, Islamic Azad University, Pishva

2 Department of Genetic and Biotechnology, Faculty of Biological Sciences,Varamin-Pishva Branch, Islamic Azad University, Pishva

3 Agricultural Research & Education Organization(AREO), Tehran, Po Box :19395-1113

Abstract

The Gorgani Cave Salamander (Paradactylodon gorganensis) is one of at risk species that only habitat of this species is a cave in Shirabad of Golestan province. In order to genetic determination of two phenotypes of Gorgan Mountain Salamander, after sampling and DNA extraction from ventral fins of 10 Salamander, the D-loop region was used. After loci amplifying in PCR, the all samples were sequenced. In this study, the results revealed that all sequence pattern of D-loop region were similar that it can be concluded that The D-loop region in the two strains was a one haplotype and there wasn’t any variation and it would appear that the high genetic similarities and no differences are in the two phenotypes of this population. 

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