اثر عصاره گیاه سالیکورنیا .Salicornia sp بر فعالیت آنزیم های کبدی و شاخص های آنتی اکسیدانی ماهی کفال خاکستری (1785 ,Mugil cephalus Linnaeus)

نوع مقاله : تغذیه

نویسندگان

1 گروه شیلات، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، چابهار، ایران

2 مرکز تحقیقات آب‌های دور، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، چابهار، ایران

چکیده

 گیاه سالیکورنیا (.Salicornia sp) یکی از گیاهانی است که جنبه ­های دارویی موثری برای آن قائل شده ­اند. هدف از مطالعه حاضر، بررسی اثر عصاره الکلی گیاه سالیکورنیا (.Salicornia sp) بر فعالیت آنزیم ­های کبدی (آسپارتات آمینوترانسفراز (AST)، آلانین آمینوترانسفراز (ALT) و آلکالین فسفاتاز (ALP)) و شاخص ­های آنتی­ اکسیدانی (سوپراکسید دیسموتاز (SOD)، گلوتاتیون پراکسیداز (GPX)، مالون ­دی آلدئید (MDA) و کاتالاز (CAT)) ماهی کفال خاکستری (Mugil cephalus) به ­مدت 60 روز می­ باشد. در این مطالعه، تعداد 240 قطعه ماهی با میانگین وزنی 0/43±8/42 گرم در یک طرح کاملاً تصادفی با 4 تیمار آزمایشی و 3 تکرار (با تعداد 20 قطعه در هر تکرار) مورد آزمایش قرار گرفتند. تیمار شاهد (بدون عصاره گیاه) و تیمارهای آزمایشی 2، 3 و 4 به ­ترتیب دارای 500، 1000 و 1500 میلی­ گرم عصاره گیاه در هر کیلوگرم عصاره گیاه در غذا بودند. در پایان آزمایش، بیش ­ترین شاخص ­های آنتی ­اکسیدانی CAT، GPX و کم ­ترین شاخص آنتی اکسیدانی MDA و آنزیم ­های ALT ،AST و ALP در تیمار حاوی 500 و 1000  میلی­ گرم بر کیلوگرم عصاره الکلی گیاه مشاهده شد که با تیمار شاهد دارای تفاوت معنی­ دار بود (0/05>P). لذا با توجه به نتایج به ­دست آمده، به­ منظور کاهش اکسیداسیون لیپید و بهبود سلامت کبد، استفاده از عصاره عصاره الکلی گباه سالیکورنیا به ­عنوان مکمل غذایی مهم برای ماهی کفال خاکستری توصیه می­ شود.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of extract of Salicornia sp. plant on liver enzymes activity and antioxidant parameters in grey mullet, Mugil cephalus Linnaeus 1758

نویسندگان [English]

  • Paria Akbary 1
  • Asma Baluch Amin 1
  • Zahra Amini Khoei 2
1 Fisheries Department, Faculty of Marine Sciences, University of Maritime and Marine Sciences Chabahar, Chabahar, Iran
2 Agricultural Research Education and Extension Organization (AREEO), Offshore Research Center, Iranian Fisheries Science Research Institute (IFSRI), Chabahar, Iran
چکیده [English]

Salicornia sp is one of the plants with applied medicinal value. This study aimed to examine the effect of Salicornia sp plant alcoholic extract on activity of hepatic enzymes (aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP)) and antioxidant parameters (Superoxidase dismutase (SOD), Glutathione peroxidase (GPX), Malondialdehyde (MDA) catalase (CAT)) in grey mullet, Mugil cephalus for 60 days. The experiment was conducted in a completely randomized design with 240 of grey mullet (with average weight of 8.42±0.43g) in 4 treatments and 3 replicates (n=20 in each replicate) and included: control group without using alcoholic extract, and another groups (treatment 2,3and 4) the amounts of this extract were 500, 1000 and 1500 mg/kg food. In the end of experiment, the highest antioxidant parameters of CAT and GPX and the lowest MDA antioxidant parameter and ALT,AST,ALP enzymes were observed in the diets containing 500 and 1000 mg /kg alcoholic extract in all of these parameters and showed a significant difference compared with control treatment (P<0.05). So, based on the results, in order to reduce lipid oxidation and improve the liver, it is recommended to use of alcoholic extract of Salicornia sp as important feed supplements for grey mullet production.

کلیدواژه‌ها [English]

  • Grey mullet
  • Salicornia plant extract
  • Free radical
  • Oxidative strss

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فصلنامه محیط زیست جانوری
دوره 12، شماره 2
تیر 1399
صفحه 169-176

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