تأثیر منابع مختلف سلنیوم بر فراسنجه‌های منی خروس‌های مادر گوشتی مسن تحت چالش ایمونولوژیک لیپو پلی ساکارید (LPS)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی، دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

3 گروه علوم دامی، دانشکده کشاورزی، دانشگاه تهران، کرج، ایران

4 گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی گلستان، گرگان، ایران

10.22034/aej.2023.423415.3053

چکیده

سلنیوم به عنوان جزیی از آنزیم‌هایی مانند گلوتاتیون پراکسیدازها و سلنوپروتئین‌ها، نقش کلیدی در بسیاری از فرآیندهای بیولوژی نظیر سیستم دفاعی آنتی‌اکسیدانی، باروری و عملکرد سیستم ایمنی دارد. لذا این پژوهش به منظور تأثیر منابع مختلف سلنیوم بر فراسنجه‌های منی خروس‌های مادر گوشتی مسن تحت چالش ایمونولوژیک لیپو پلی ساکارید (LPS ) انجام شد. پژوهش حاضر با استفاده از 96 قطعه خروس مادر گوشتی راس 308 و سن 45 هفته درقالب آزمایش فاکتوریل 2×3 با 8 گروه آزمایشی و با 4 تکرار و 3 پرنده در هر تکرار انجام شد. سه منبع مختلف سلنیوم شامل سلنیوم معدنی(سلنیت سدیم)، سلنیوم آلی (سلمکس) و نانو بایوکیلات سلنیومُ (بن‌داسل) به جیره افزوده شدند. در زمان صفر قبل از تزریق و پس از آخرین تزریق LPS در پایان آزمایش، انزال خروس‌های هر تکرار جمع آوری و مورد ارزیابی قرار گرفت. شاخصه‌های مورد ارزیابی شامل میزان MDA منی، ظرفیت آنتی‌اکسیدانی کل ،فعالیت گلوتاتیون پراکسیداز پلاسمای منی و غلظت تستوسترون در پلاسمای خون بود. تجزیه و تحلیل داده‌های پیوسته حاصل با استفاده از رویه‌های‌ GLM و MIXED و نرم افزار SAS 9.4 (2012) انجام شد. ظرفیت آنتی‌اکسیدانی کل در گروه شاهد دریافت کننده لیپو‌پلی‌ساکارید (LPS) به طور معنی‌داری کمتر از گرو‌ه‌های سلمکس و بن‌داسل دریافت کننده PBS بود، اما با سایر گروه‌ها تفاوتی نداشت (0/05<P). فعالیت گلوتاتیون پراکسیداز در گروه شاهد دریافت کننده LPS نسبت به گروه سلمکس دریافت کننده PBS کمتر بود اما با سایر گروه‌ها تفاوتی مشاهده نشد و کمترین مقدار MDA در گروه سلمکس دریافت کننده PBS مشاهده شد که تفاوت معنی‌داری با گروه‌های شاهد و سلنیت سدیم دریافت کننده LPS داشت. غلظت تستوسترون در گروه‌های بن‌داسل و سلنیت سدیم دریافت کننده PBS به طور معنی‌داری بیش تر از گروه شاهد دریافت کننده LPS بود، اما بین سایر تیمارها تفاوتی مشاهده نشد. بر اساس نتایج حاصل از این پژوهش، به‌نظر می‌رسد افزودن سلنیوم آلی از منابع سلمکس و بن‌دا‌سل و به مقدار 0/30 میلی‌گرم در کیلوگرم می‌تواند اثرات منفی افزایش سن در خروس‌ها کاهش و موجب بهبود عملکرد تولیدمثلی شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effects of different selenium sources on semen quality of aged broilers under lipopolysaccharide (LPS) stress

نویسندگان [English]

  • Morteza Asghari Moghadam 1
  • Seyed Reza Hashemi 1
  • Mehran Mehri 2
  • Amir Karamzadeh Dehaghani 3
  • Homa Davoodi 4
1 Department of Animal Science, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Department of Animal Science, Faculty of Agriculture, Zabol University, Zabol, Iran
3 Department of Animal Science, Faculty of Agriculture, University of Tehran, Karaj, Iran
4 Department of Immunology, Faculty of Medical Science, Golestan University of Medical Science, Gorgan, Iran
چکیده [English]

Selenium, a vital element in the body, plays a crucial role in the functioning of enzymes related to antioxidant defense, fertility, and the immune system. This study seeks to explore how various selenium sources impact the semen quality of aging broilers under lipopolysaccharide stress (LPS). A 2×3 factorial experiment involved 96 Ross 308 strain roosters at 45 weeks of age, organized into four replications of three birds each. The experiment included three selenium sources: mineral selenium (sodium selenite), organic selenium, and nano-bio chelate of selenium, added to the diet. Semen was assessed before LPS injection and after the final LPS injection at the experiment's conclusion. Evaluation indicators comprised malondialdehyde (MDA) concentration in semen, total oxidative capacity, glutathione peroxidase activity in semen, and testosterone levels in blood plasma. Statistical analysis utilized SAS 9.4 through GLM or MIXED procedures.
The total antioxidant capacity and glutathione peroxidase activity levels exhibited variations among the groups. Specifically, in the LPS-receiving group, the control group demonstrated significantly lower total antioxidant capacity compared to the Selmax and Bendacel groups that received PBS. Additionally, the control group exhibited lower glutathione peroxidase activity compared to the Selmax group that received PBS, although no differences were observed between this group and others. Moreover, the Selmax group that received PBS displayed the lowest amount of MDA, a significant difference from the control and sodium selenite groups that received LPS. Notably, the testosterone concentration in birds that received Bendacel and sodium selenite, and were challenged with PBS, was significantly higher than in birds that received LPS without any source of selenium. The study demonstrated that using organic selenium from Selmax and Bendacel sources has the potential to enhance the reproductive performance of aged roosters, mitigating the adverse effects of aging. Notably, the effective addition of 0.30 mg/kg of organic selenium emerged as a positive intervention in improving reproductive outcomes in the study's context.

کلیدواژه‌ها [English]

  • Rooster
  • Selenium
  • Testosterone
  • Semen
  • Lipopolysaccharide

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فصلنامه محیط زیست جانوری
دوره 15، شماره 3
آبان 1402
صفحه 137-144

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