بررسی اثر بازدارنده عصاره های متانولی، استونی و آبی استخراج شده از صدف ملوک (Solen vagina) بر رشد باکتری ویبریو هاروی (Vibrio harveyi) در پست لاروهای میگوی وانامی (Litopenaeus vannamei)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، واحد بندرعباس، دانشگاه آزاد اسلامی، بندرعباس، ایران

2 گروه شیلات، مرکز تحقیقات فناوری های دریایی و شیلاتی، واحد بندرعباس، دانشگاه آزاد اسلامی، بندرعباس، ایران

3 موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

10.22034/aej.2022.374111.2908

چکیده

هدف از این مطالعه مقایسه قطر هاله (mm) ایجاد شده ناشی از عدم رشد باکتری ویبریو هاروی در پست لاروهای میگو وانامی (Litopenaeus vannamei) تحت تاثیر عصاره های متانولی، استونی و آبی (ppm) استخراج شده از صدف ملوک (S. vagina) می باشد. عصاره های متانولی، استونی و آبی با غلظت‌های 100، 150، 200، 250، 300، 350، 400 و 450 ppm برای ارزیابی MIC استفاده شدند. بر اساس نتایج بدست آمده، عصاره متانولی استخراج شده از صدف ملوک رشد باکتری ویبریو هاروی را در غلطت 450 ppm متوقف نمود. اگرچه استفاده از این عصاره سبب توقف رشد باکتری در غلظت 450 ppm شد، اما قطر هاله ایجاد شده در مقایسه با هاله عدم رشد ناشی از آنتی بیوتیک کم تر بود. عصاره استونی صدف از رشد باکتری ویبریو هاروی در غلظت 200ppm و غلظت های بالاتر جلوگیری نمود و هاله عدم رشدی به قطر 4.2 میلی متر ایجاد نمود. هم چنین عصاره آبی فاقد هر گونه اثر بازدارنده ای بود. نتایج مربوط به آزمون مقایسه میانگین ها نشان داد که بین غلظت های مختلف عصاره های متانولی و استونی استخراج شده اختلاف معنی داری وجود داشت (P<0/05). هم چنین بین غلظت های موثر مشابه تیمارهای مختلف (200، 250، 300، 350، 400 و 450 ppm) اختلافات معنی داری مشاهده شد (P<0/05). بنابراین، عصاره های متانولی و استونی S. vagina را می توان به عنوان یک ماده زیستی فعال برای جلوگیری از بیماری ویبریوز در Litopenaeus vannamei استفاده نمود. با این حال، مطالعات بیش تری برای بررسی سایر عصاره های قابل استخراج از این گونه و اثرات آن ها پیشنهاد می گردد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The inhibitory effect of methanolic, acetone, and aqueous extracts of razor clam (Solen vagina) on the growth of Vibrio harveyi bacteria in post-larvae of Vannamei shrimp (Litopenaeus vannamei)

نویسندگان [English]

  • Shahrzad Foroutan 1
  • Amir Houshang Bahri 2
  • Babak Ghaednia 3
  • Flora Mohammadizadeh 2
  • Maryam Mirbakhsh 3
1 Department of Fisheries, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, Iran
2 Department of Fisheries, Marine and Fisheries Technologies Research Center, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, Iran
3 Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization ,Tehran, Iran
چکیده [English]

This study was aimed to compare the halo diameter (mm) caused by the lack of bacteria growth (Vibrio harvey) in the post-larvae of Vannamei shrimp (Litopenaeus vannamei) under the influence of methanolic, acetone, and aqueous extracts (ppm) of razor clam (Solen vagina). Based on the obtained results, the methanolic extract stopped the growth of Vibrio harvey bacteria at 450 ppm. Although the use of this extract stopped the growth of bacteria at a concentration of 450 ppm, the diameter of the created halo was smaller compared to the halo of non-growth caused by antibiotics. Acetone extract prevented the growth of Vibrio harvey bacteria at 250 ppm and higher concentrations and created a lack of growth halo with a diameter of 2.8 cm. Also, the aqueous extract did not have any inhibitory effect. The results of the mean comparison test showed that there was a significant difference between different concentrations of methanolic and acetone extracts (P<0/05). Also, significant differences were observed between the same effective concentrations of different treatments (250, 300, 350, 400 and 450 ppm) (P<0/05). Therefore, methanolic and acetone extracts of S. vagina can be used as a bioactive substance to prevent vibriosis in Litopenaeus vannamei. However, more studies are suggested to investigate other extracts of this species and their effects.

کلیدواژه‌ها [English]

  • Razor clam
  • Extracts
  • Vibrios
  • Vannamei shrimp
  • Acetone extract

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فصلنامه محیط زیست جانوری
دوره 15، شماره 3
آبان 1402
صفحه 169-174

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