تاثیر نانوذرات نقره بر سمیت جیوه در آرتمیا (Artemia salina (Linnaeus, 1758 در مواجهه همزمان و جداگانه

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران

2 گروه شیلات، دانشکده منابع طبیعی، دانشگاه کردستان، سنندج، ایران

10.22034/aej.2023.387227.2937

چکیده

استفاده روزافزون از نانوذرات در صنایع مختلف موجب شده است که مقادیر بسیار بالایی از این دسته مواد در محیط پیرامون آزاد شوند و بنابراین گسترش آن در طبیعت احتمال تأثیر آن‌ها بر جانداران را افزایش داده است. در همین راستا اثر مواجهه هم‌زمان و جداگانه نانوذرات نقره، نیترات نقره و یون جیوه بر سخت‌پوست Artemia salina مورد بررسی قرار گرفت. بدین منظور، سیست گونه A. salina در محیط آزمایشگاهی تفریخ شد و ناپلی A. salina در معرض غلظت‌های مختلف 0/0001، 0/001، 0/005، 0/01 و 0/1 میلی گرم در لیتر یون جیوه به طور جداگانه و هم چنین با غلظت ثابت 0/1 میلی گرم در لیتر نانوذرات نقره یا نیترات نقره به طور هم زمان قرار گرفت و میزان مرگ و میر آن پس از زمان‌های 24 و 48 ساعت بررسی شد. هم چنین اثرات این آلاینده‌ها بر پروفایل اسید چرب A. salina مورد سنجش قرار گرفت. میزان تلفات A. salina در رویارویی هم زمان جیوه و نیترات نقره و رویارویی هم زمان جیوه و نانوذرات نقره به دلیل واکنش جیوه با نیترات و نانوذرات نقره، کاهش یافته و اثر سمیت جیوه کم شد. هم چنین مقایسه ترکیب اسید چرب A. salina در تیمارهای مختلف نشان داد که اسیدهای چرب اشباع در رویارویی های هم‌زمان جیوه با نیترات و نانوذرات نقره نسبت به رویارویی جداگانه جیوه،کاهش یافته است. علاوه بر این، مقدار اسید‌های چرب غیر اشباع تک زنجیره در رویارویی هم زمان جیوه و نیترات نقره به طور معنی‌داری بیش تر از شاهد بود (0/05>P). در مجموع، اثرات سمیت یون جیوه بر آرتمیا در رویارویی هم زمان با نانوذرات و یون نقره نسبت به رویارویی جداگانه کاهش یافت که موید توجه بیش تر به پتانسیل برهم کنش آلاینده ها در اکوسیستم‌های آبی و اثر این برهم کنش بر دسترسی زیستی و سمیت آلاینده‌ها می‌باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of silver nanoparticles on the toxicity of mercury in Artemia salina (Linnaeus, 1758) in simultaneous and individual exposure

نویسندگان [English]

  • Narges Ravantab 1
  • Zahra Ghasemi 1
  • Seyed Ali Johari 2
1 Department of Fisheries, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas, Iran
2 Department of Fisheries, Faculty of Natural Resources, University of Kurdistan, Sanandaj, Iran
چکیده [English]

The use of nanoparticles in various industries has led to the release of very large amounts of these materials in the environment, and therefore, its spread in nature has increased the likelihood of their impact on organisms. In this study, the simultaneous and separate effects of mercury ions with/without silver nitrate and silver nanoparticles on Artemia salina were investigated. For this purpose, A. salina was grown in vitro and A. salina nauplius was exposed to different concentrations of silver nanoparticles and mercury ions and its mortality rate was evaluated after 24 and 48 hours. The effects of these contaminants on the fatty acid profile of A. salina were also evaluated. A. salina losses were reduced by simultaneous exposure of mercury and silver nitrate and simultaneous exposure of mercury and silver nanoparticles due to the mercury uptake by silver nitrate and silver nanoparticles, and the effect of mercury toxicity was reduced and mercury caused fewer losses in simultaneous exposure. Also, in the study of A. salina fatty acid profile in comparison with separate mercury treatment, a decrease in SFA fatty acids in the simultaneous exposure of mercury and silver nitrate to the amount of this type of fatty acids in the control was observed. In addition, the amount of MUFA fatty acids in the simultaneous exposure of mercury and silver nitrate was significantly higher than the control (P˂0.05). In conclusion, toxicity effects of mercury on the artemia was reduced in simultaneous exposure to silver ions and nanoparticles compared with the separate exposure, indicating the necessity of paying more attention to the contaminants interactions in aquatic ecosystems and the effects of this interaction on the contaminants bioavailability and toxicity.

کلیدواژه‌ها [English]

  • NNanotoxicology
  • Heavy metal
  • Mercury
  • Contaminants interaction
  • Artemia salina
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