ارتباط جایگاه های T340C و G286A ژن GnRHR با صفات کیفی و کمی اسپرم گاوهای هلشتاین ایران

نوع مقاله : ژنتیک

نویسندگان

گروه علوم دامی، دانشکده کشاورزی و دامپزشکی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران

چکیده

این پژوهش به ­منظور بررسی ارتباط جایگاه ­های G286A و T340C ژن گیرنده هورمون آزاد‌ کننده ‌گنادوتروپین (GnRHR) و صفات مرتبط با کیفیت اسپرم در جمعیت گاوهای نر هلشتاین انجام گرفت. اطلاعات فنوتیپی و نمونه ­های خون از 150 راس گاو نر موجود در دو مرکز اصلاح نژاد شیخ حسن تبریز و جاهد کرج بین سال­ های 1395-1368 جمع­ آوری شدند. جهت شناسایی چند‌شکلی‌ های تک نوکلئوتیدی (SNP) ژن GnRHR از روش PCR-RFLP و آنزیم‌ های برشی MboII و BspMI به ­ترتیب برای جایگاه­ های G286A و T340C استفاده گردید. در جایگاه T340C دو آلل T وC و در جایگاه G286A دو آلل A و G به ­دست آمد. آلل ­های G) 0/679) و T) 0/676) و ژنوتیپ­ های TC) 0/46) و GA) 0/45) بیش ­ترین فراوانی را داشتند. نتایج تجزیه واریانس، ارتباط معنی‌ دار جایگاه­ های کاندیدای فوق را با اکثر صفات اسپرم نشان داد. نتایج مقایسه میانگین حاکی از برتری ژنوتیپ ­های هتروزیگوت GA  و TC  در جایگاه ­های مورد بررسی داشت. این مطالعه برای اولین بار ارتباط معنی ­دار ژن GnRHR را با صفات مرتبط با اسپرم در جمعیت گاوهای هلشتاین ایران بررسی نمود و نتایج حاکی از آن است که GnRHRدر فرآیندهای مختلف تولید اسپرم نقش دارد. با این­ حال، تحقیقات بیش ­تر ضروری است.

کلیدواژه‌ها

عنوان مقاله [English]

Association of T340C and G286A loci GnRHR gene polymorphism with sperm quality and quantity in Iranian Holstein bulls

نویسندگان [English]

  • Mohamadtaghi Zarinnia
  • Abolfazl Gorbani
Animal Science department, Faculty of agriculture and veterinary, Shabestar Branch, Is-lamic Azad university, Shabestar, Iran
چکیده [English]

This study was performed to investigate the relationship between G286A and T340C loci of the gonadotropin releasing hormone receptor (GnRHR) gene and sperm quality related traits in Iranian Holstein bull’s populations. Phenotypic data and blood samples were collected from 150 bulls at the Sheikh Hassan Tabriz and Jahed Karaj Breeding Centers between 1992-2014. To identify single nucleotide polymorphisms (SNP) of GnRHR gene, PCR-RFLP method and MboII and BspMI restriction enzymes were used for G286A and T340C loci, respectively. The T and C alleles in T340C locus and A and G alleles in G286A locus two were obtained. G (0.679) and T (0.676) alleles and TC (0.46) and GA (0.45) genotypes were the most abundant. Analysis of variance showed a significant relationship between the above candidate loci and most of the sperm traits. The results of mean comparison indicated the superiority of heterozygous GA and TC genotypes in the studied loci. This study for the first time investigated the significant relationship of GnRHR gene with sperm-related traits in Iranian Holstein population. However, further research is needed.

کلیدواژه‌ها [English]

  • Holstein
  • GnRHR
  • Sperm trait
  • Polymorphism
  • Bull

مراجع

  1. Brito, L.F.; Silva, A.E.; Rodrigues, L.H.; Vieira, F.V.; Deragon, L.A. and Kastelic, J.P., 2002. Effects of environmental factors, age and genotype on sperm production and semen quality in Bus indicts and Bus Taurus AI bulls in Brazil.  Animal Reproduction Science. Vol. 70, No. 3-4, pp: 181-190.
  2. Cheng, C.K. and Leung, P.C.K., 2005. Molecular biology of gonadotropin- releasing hormone (GnRH)-1 and GnRH-11 and their receptors in humans. Endocrine Reviews. Vol. 25, No. 11, pp: 1-108.
  3. Dai, L.; Zhao, Z.; Zhao, R.; Xiao, S.; Jiang, H.; Yue, X.; Li, X.; Gao, Y.; Liu, J. and Zhang, J., 2009. Effects of novel single nucleotide polymorphisms of the FSH beta-subunit gene on semen quality and fertility in bulls. Animal Reproduction Science. Vol. 114, No. 1-3, pp: 14-22.
  4. Fortes, M.R.; Reverter, A.; Nagaraj, SH.; Zhang, Y.; Jonsson, NN.; Barris, W.; Lehnert, S.; BoeHansen, G.H. and Hawken, J., 2011. A single nucleotide polymorphism derived regulatory gene network underlying puberty in 2 tropical breeds of beef cattle. Journal of Animal Science.  Vol. 89, No. 6, pp: 1669-1683.
  5. Gadea, J., 2005. Sperm factors related to in vitro and in vivo porcine fertility. Theriogenology. Vol. 63, No. 2, pp: 431-444.
  6. Gorbani, A.; Vaez Torshizi, R.; Bonyadi, M. and Amirinia, S., 2009. A MPs PCR-RFLP within bovine growth hormone gene and its association with sperm quality traits in Iranian Holstein bulls. African journal of Biotechnology. Vol. 8. No. 19, pp: 4811-4816.
  7. Huang, S.Y.; Chen, M.Y.; Lin, E.C.; Tsou, H.L.; Kuo, H.Y.; Ju, C.C. and Lee, W.C., 2002. Effects of single nucleotide polymorphisms in the 5′-flanking region of heat shock protein 70.2 gene on semen quality in boars. Animal Reproduction Science. Vol. 70, pp: 99-109.
  8. Huhtaniemi, I. and Alevizaki M., 2007. Mutations along the hypothalamic-pituitary–gonadal axis affecting male reproduction. Reproductive BioMedicine Online. Vol. 15, No. 6, pp: 622-632.
  9. Li, G.; Wu, H.P.; Fu, M.Z. and Zhou, Z.Q., 2011. Novel single nucleotide polymorphisms of GnRHR gene and their association with litter size in goats. Archive Tierzucht. Vol. 54, No. 6, pp: 618-624.
  10. Lin, C.L.; Ponsuksili, S.; Tholen, E.; Jennen, D.I.G.; Schellander, K. and Wimmers, K., 2006. Candidate gene markers for sperm quality and fertility of boar. Animal Reproduction Science. Vol. 92, pp: 349-363.
  11. Liron, J.P.; Prando, A.J.; Fernandez, M.E.; Ripoli, M.V.; Rogberg-Munoz, A.; Goszczynski, D.E.; Posik, D.M.; Peral-Garcia, P.; Balbo, A. and Giovambattista, G., 2012. Association between GnRHR, LHR and IGF1 polymorphisms and timing of puberty in male Angus cattle. BMC Genetics.  Vol. 2156, pp: 13-26.
  12. Iyer, K.B.; Ingawale, M.V.; Kuralkar, S.V.; Bankar, P.S.; Sajid Ali, S.; Ingole, R.S.; Hajare S.W. and Pawshe, C.H., 2019. Association of GnRH receptor gene polymorphism on testicular parameters and semen quality in HF crossbred bulls. Indian Journal of Animal Research. Vol. 53, No. 9, pp: 1140-1143.
  13. Kumar, S.; Ganguly, I.; DEB, R.; Singh, U.; Mandal, D.; Mann, S.; Singh, R.; Kumar, M. and Sharma, A., 2015. Elucidating the role of bovine GnRHR on semen quality traits among crossbred bull. The Indian journal of animal sciences. Vol. 85, No. 12, pp: 1314-1317.
  14. Mathevon, M.; Buhr, M.M. and Dekkers, J.C.M., 1998. Environmental, management and genetic factors affecting semen production in Holstein bulls. Journal of dairy Science. Vol. 81, pp: 3321-3330.
  15. Milazzotto, M.P.; Rahal, P.; Nichi, M.; Miranda-Neto, T.; Teixeira, L.A.; Ferraz, J.B.S.; Eler, J.P.; Campagnari, F. and Garcia, J.E., 2008. New molecular variants of hypothalamus-pituitary-gonad axis genes and their association with early puberty phenotype in Bos Taurus indicts (Nellore). Livestock Science. Vol. 114, pp: 274-279.
  16. Nazi, R. and Sellamuthu, R., 2006.  Receptors in Spermatozoa: Are they real? Journal of Andragogy. Vol. 27: pp: 627-636.
  17. Rothschild, M.F. and Bidanel, J.P., 1998. Biology and genetics of reproduction. In: Rothschild, M.F., (Ed) The genetics of the pig. CAB International, Wallingford. pp: 313-343.
  18. Saremi, M.; Saremi, A.M. and Tavallaei, M., 2008. Rapid genomic DNA extraction (RGDE). Forensic Science International: Genetics Supplement Series. Vol. 1, pp: 63-65.
  19. Weinbauer, G.F.; Luetjens, C.M.; Simoni, M.; Nieschlag, E.; Ni-eschlag, E.; Behre, H.M. and Nieschlag, S., 2010. Physiology of testicular function in Andragogy Springer.   Berlin. Vol. 10, pp: 11-59.
  20. Wimmers, K.; Lin, C.L.; Tholen, E.; Jennen, D.G.; Schellander, K. and Ponsuksili, S., 2005. Polymorphisms in candidate genes as markers for sperm quality and boar fertility. Animal Genetic. Vol. 36, pp: 152-155.
  21. Yang, W.C.; Tang, K.Q.; Yu, J.N.; Zhang, C.Y.; Zhang, X.X. and Yang, L.G., 2011. Effects of MboII and BspMI polymorphisms in the gonadotropin releasing hormone receptor (GnRHR) gene on sperm quality in Holstein bulls. Molecular Biology Reports. Vol. 38, No. 5, pp: 3411-3415.
  22. Yang, W.U.; Tang, K.Q.; Zhang, C.H.; Deqing, X.U.; Wen, Q.U. and Yang, L.I.I., 2011. Polymorphism of the GnRHR gene and its association with litter size in Boer goats. South African Journal of Animal Science.  Vol. 4, pp: 41-49.
فصلنامه محیط زیست جانوری
دوره 12، شماره 1
فروردین 1399
صفحه 73-78

فایل ها

هم رسانی

ارجاع به این مقاله

آمار