بررسی زهراگینی جدایه های مختلف قارچMetarhizium anisopliae استخراج شده از خاک باغات مختلف آذربایجان شرقی روی بید آرد (Anagasta kuehniella)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاه‌پزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

2 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

چکیده

قارچ‌های بیماری‌گر حشرات از جمله عوامل کنترل بیولوژیک هستند که به‌صورت طبیعی جمعیت حشرات را در سطح تعادل نگاه می‌دارند و به‌عنوان حشره‌کش‌های میکروبی مورد استفاده قرار می‌گیرند. در این تحقیق، جدایه‌های مختلف قارچ‌های بیمارگر حشرات، از خاک باغات مناطق مختلف آذربایجان شرقی جداسازی و بیماری‌زایی آن ها علیه بید آرد Anagasta kuehniella (Lep.:Pyralidae) بررسی شد. از بیست نمونه‌ مختلف خاک جمع‌آوری شده، پنج جدایه قارچ بیمارگر Metarhizium anisopliae، با استفاده از روش طعمه‌گذاری با لارو بید آرد A. kuehniella جداسازی و شناسایی شد و سپس زیست سنجی لاروهای سن چهارم، به‌روش غوطه‌ورسازی با جدایه‌ها در پنج غلظت، در سه تکرار و با دوازده عدد لارو بید آرد انجام شد. نتایج زیست سنجی نشان داد که لاروهای سن چهارم بید آرد به همه جدایه‌ها حساس بودند. بیش ترین مرگ و میر به وسیله جدایه MTA با حدود 90 درصد روی میزبان بید آرد A. kuehniella  بوده است. که در بالاترین گروه زهرآگینی قرار گرفتند. جدایه‌ MTA با کم‌ترین LC50 با مقدار 106×0/42 کنیدی بر میلی‌لیتر، بیش ترین زهرآگینی را نسبت به سایر جدایه‌ها داشته ‌و بیش ترین LC50 ثبت شده مربوط به جدایه MTV، با مقدار 109×4/3 کنیدی بر میلی‌لیتر بود، که کم ترین زهراگینی را نسبت به سایر جدایه‌ها داشته است. مقایسه میانگین و گروه‌بندی جدایه‌ها نشان داد که بین جدایه‌ها و اثربخشی غلظت‌های مختلف تفاوت معنی‌داری وجود دارد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Toxicity study of different isolates of Metarhizium anisopliae extracted from the soil of different orchards of East Azarbaijan on flour moth (Anagasta kuehniella)

نویسندگان [English]

  • Mohadeseh Moghasem 1
  • Manijeh Jamshidi 1
  • Reza Khakvar 2
  • Sevil Nematollahi 1
1 Department of Plant Protection, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Department of Plant Protection, Faculty of Agriculture, Tabriz University, Tabriz, Iran
چکیده [English]

Entomopathogenic fungi are the biocontrol agents which naturally keep the insect population in balance and can be used as microbial insecticides. In the present study, some isolates of entomopathogenic fungi were isolated from different soil samples and their virulence was investigated against flour moth, Anagasta kuehniella Zeller (Lep: Pyralidae). Five isolates M. anisopliae from Twenty isolates were isolated, using soil-baiting method from collected soil samples in different gardens East Azarbaijan were identified. The flour moth, A. kuehniella were infected using dipping method with isolates were performed at 5 concentrations, in 3 replications and with 12 of flour moth. The results showed that the instars of fourth larval instars of flour moth were susceptible to all isolates of M. anisopliae. The most virulent isolates were MTA with approximately 90% mortality on A. kuehniella. The most virulent isolates were MTA with 0.42×106 conidia/ml LC50 on A. kuehniella while the least virulent isolate were MTV 4.3×109 conidia/ml LC50 on A. kuehniella. Comparison of means and grouping of isolates showed that there was a significant difference between M. anisopliae isolates and the effectiveness of different concentrations.

کلیدواژه‌ها [English]

  • Flour moth
  • Microbial control
  • Isolation
  • Virulence
  • Metarhizium anisopliae

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فصلنامه محیط زیست جانوری
دوره 14، شماره 2
تیر 1401
صفحه 387-394

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