تاثیر باکتری های پروبیوتیکی جدا شده از روده ماهیان استان های خوزستان، بوشهر و هرمزگان بر سیستم استرس اکسیداتیو در بافت کبد و سرم خون ماهیان باس دریایی آسیایی (Lates calcarifer) پرورش یافته در استخرهای خاکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران

2 مرکز تحقیقات تکثیر و پرورش آبزیان، گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران

10.22034/AEJ.2021.310091.2661

چکیده

پروبیوتیک ها باکتری هایی هستندکه در مقادیر کافی دارای اثرات سودمند برای میزبان می باشند. امروزه استفاده از پروبیوتیک ها به عنوان مکمل غذایی در صنعت آبزی پروی رشد فزاینده ای داشته است. هدف از این تحقیق بررسی اثر پروبیوتیک های مختلف بر روی فاکتورهای آنتی اکسیدانی کبد و سرم خون ماهی سی باس آسیایی (Lates calcarifer) بود. بدین منظور، ماهیان سی باس آسیایی با وزن اولیه 8/79±171/29 گرم، به مدت 60 روز با به کارگیری چهار جیره شامل گروه شاهد (بدون باکتری)، گروه اول (سویه انتخابی جدا شده از ماهیان استان خوزستان)، گروه دوم (سویه انتخابی جدا شده از ماهیان استان بوشهر) و گروه سوم (سویه انتخابی جدا شده از ماهیان استان هرمزگان)، مورد تغذیه قرار گرفتند. نمونه برداری از ماهیان در روزهای صفر، 30 و 60 آزمایش انجام شد. نتایج نشان داد که بعد از 60 روز مصرف پروبیوتیک مقادیر سوپراکسید دیسموتاز کبد در تمام گروه ها کاهش و مقادیر گلوتاتیون در گروه های دوم و سوم افزایش معنی داری نسبت به گروه شاهد داشته است (0/05>p). هم چنین مقادیر سوپراکسید دیسموتاز سرم خون در تمام گروه ها و مقادیر گلوتاتیون در گروه اول افزایش معنی داری نسبت به گروه شاهد داشته است (0/05>p). نتایج این مطالعه نشان داد که سویه انتخابی جدا شده از ماهیان استان خوزستان، بوشهر و هرمزگان می تواند در بهبود شاخص های آنتی اکسیدانی کبد و سرم ماهی سی باس آسیایی نقش داشته باشد.
 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of probiotic bacteria isolated from fish intestines of Khuzestan, Bushehr and Hormozgan provinces on oxidative stress system in liver tissue and blood serum of Asian sea bass (Lates calcarifer)

نویسندگان [English]

  • Razieh Sokooti 1
  • Mojdeh Chelemal Dezfoulnejad 1 2
  • Mehran Javaheri baboli 1 2
  • Abolfazl Askary Sary 1 2
  • Hadideh Mabudi 1 2
1 Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
2 Aquaculture Research Center, Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
چکیده [English]

Probiotics are bacteria that in sufficient quantities have beneficial effects on the host. Today, the use of probiotics as food supplements in the aquatic industry has grown exponentially. The aim of this study was to investigate the effect of various probiotics on antioxidant factors in the liver and blood serum of Asian sea bass (Lates calcarifer). For this purpose, Asian bass fish with an initial weight of 171.29±8.79 g, for 60 days using four diets including control group (without bacteria), the first group (selected strain isolated from fish in Khuzestan province), the second group (selected strain isolated from fish of Bushehr province) and the third group (selected strain isolated from fish of Hormozgan province) were fed. Fish sampling was performed on days 0, 30, and 60 of the experiment. The results showed that after 60 days of probiotic use, liver superoxide dismutase levels decreased in all groups and glutathione levels in the second and third groups increased significantly compared to the control group (p<0.05). Also, serum superoxide dismutase levels in all groups and glutathione levels in the first group increased significantly compared to the control group (p<0.05). The results of this study showed that the selected strain isolated from fish in Khuzestan, Bushehr, and Hormozgan provinces can improve the antioxidant indices of the liver and serum of Asian seabass.

کلیدواژه‌ها [English]

  • Dietary probiotic
  • Superoxide dismutase
  • Catalase
  • Glutathione
  • Malondialdehyde
  • Asian sea bass

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فصلنامه محیط زیست جانوری
دوره 14، شماره 3
آبان 1401
صفحه 285-292

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