ردیابی مولکولی سیرکوویروس های کبوتر در نمونه های کبدی کبوتران تلف شده مشکوک به عفونت سیرکوویروسی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 بهداشت و بیماری های طیور، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

3 گروه علوم آزمایشگاهی، دانشکده پیراپزشکی، دانشگاه علوم پزشکی جندی شاپور اهواز، اهواز، ایران

4 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

10.22034/AEJ.2021.318463.2700

چکیده

سندرم بیماری کبوتران جوان (YPDS) اختلالی است که منجر به تلفات در  کبوتران خانگی و زینتی به خصوص بعد از مسابقه می شود. معمولاً کبوتران جوان کم تر از یک سال تحت تاثیر قرار می گیرند و علایم غیراختصاصی مانند بی اشتهایی، اسهال و تجمع آب و غذا در چینه دان را نشان می دهد. اخیرا سیرکوویروس کبوتر (PiCV) به عنوان یکی از عوامل بالقوه در ایجاد سندرم بیماری کبوتران جوان (YPDS) در نظر گرفته می شود. در این مطالعه که برای اولین بار در  استان خوزستان  انجام شد، 16 نمونه کبدی از 16 قطعه کبوتر تلف شده در سطح شهر اهواز، جمع­ آوری شد. علایم بالینی پرندگان بیمار در گله  شامل بی ­حالی، بی ­اشتهایی، کاهش وزن همراه با اسهال آبکی سبز و زرد رنگ بود. میکروتیوب­ های حاوی نمونه های کبدی به صورت جداگانه به آزمایشگاه ارسال شد.  DNA نمونه ­ها، جهت تست PCR استخراج شد. جهت شناسایی سیرکوویروس های کبوتری از آزمون nested-PCR استفاده شد. نتایج این تحقیق نشان داد که در 10 نمونه کبدی کبوتران تلف شده (62/5 درصد)، سیرکوویروس ردیابی شد که این مطالعه، اولین ردیابی مولکولی PiCV در  نمونه های کبدی کبوتران تلف شده در اهواز می ­باشد. با توجه به آلودگی کبوتران شهر اهواز به PiCV، این موضوع باید در هنگام معاینات بالینی کبوترانی که دارای علائمی مانند مرگ ناگهانی، استفراغ، اسهال آبکی حاد و کاهش وزن هستند، مورد توجه قرار گیرد تا از بیماری های مشابه تشخیص تفریقی داده شود و درمان های حمایتی لازم اعمال گردد و از درمان­ های غیر سودمند پیشگیری به عمل آید.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Molecular detection of pigeon circoviruses in liver samples of dead pigeons suspected of circovirus infection

نویسندگان [English]

  • Hadi Shabani 1
  • Forough Talazade 2
  • Gholam Abbas Kaydani 3
  • masoudreza seyfi abad shapouri 4
1 Poultry Diseases and Health, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Department of Laboratory Sciences, School of Allied Medical Sciences Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran
چکیده [English]

Young Pigeon Disease Syndrome (YPDS) is a disorder that results in deaths in domestic and ornamental pigeons, especially after the race. Young pigeons are usually less than one year old are affected and show nonspecific symptoms such as anorexia, diarrhea, and accumulation of water and food in the crop. Pigeon circovirus (PiCV) has recently been suggested as a potential contributor to YPDS. In this study, which was performed for the first time in Khuzestan province, 16 liver samples were collected from 16 dead pigeons in Ahvaz. The clinical symptoms of sick birds in the herd included lethargy, anorexia, weight loss with watery green and yellow diarrhea. Microtubes containing liver samples were sent to the laboratory separately. DNA was extracted for the PCR test. Nested-PCR test was used to identify pigeon circoviruses. The results of this study showed that circovirus was detected in 10 liver samples of dead pigeons (62.5%), which is the first molecular detection of PiCV in liver samples of dead pigeons in Ahvaz. Due to the infection of pigeons in Ahvaz city with PiCV, this issue should be considered during clinical examinations of pigeons that have symptoms such as sudden death, vomiting, acute watery diarrhea, and weight loss, to be differentiated from similar diseases and to use fair supportive medicine and prevent useless treatments.

کلیدواژه‌ها [English]

  • Circovirus
  • Pigeon
  • Liver sample
  • Ahvaz
  • PCR

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فصلنامه محیط زیست جانوری
دوره 14، شماره 3
آبان 1401
صفحه 127-132

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