شناسایی مولکولی گونه‌های آناپلاسما بوویس و آناپلاسما مارژیناله در گاوهای استان گیلان

نوع مقاله : مقاله پژوهشی

نویسندگان

بخش تحقیقات بیماری‌های انگلی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

10.22034/AEJ.2022.324186.2727

چکیده

جنس آناپلاسما، شامل گونه‌های از تک‌یاخته‌های، درون‌سلولی است که می‌توانند بر سلامت انسان و حیوان تأثیر بگذارند. آناپلاسموزیس، در مناطق گرمسیری و نیمه گرمسیری دنیا از اهمیت اقتصادی ویژه‌ای برخوردار هستند. این تک‌یاخته منتقله توسط بندپایان همگی سلول‌های خونی میزبان‌های یوکاریوتی را آلوده می‌کنند و منحصراً در واکوئول های داخل سیتوپلاسمی سلول مستقر می‌شوند. هدف این مطالعه شناسایی مولکولی گونه‌های آناپلاسما بوویس و آناپلاسما مارژیناله در گاوهای استان گیلان بود. این پژوهش، در طول سال 1397 روی تعداد 200 نمونه خون جمع‌آوری‌شده از گاوهای نقاط مختلف استان گیلان انجام گردید. ابتدا DNA از نمونه‌های‌ خون استخراج شد و با استفاده از روش nested-PCR در مرحلۀ اول قطعه 1468 جفت بازی از ژن S rRNA 16 جنس آناپلاسما با جفت پرایمر اختصاصی تکثیر شد. سپس با استفاده از محصول PCR اولیه، قطعه 345 جفت بازی توسط جفت پرایمری اختصاصی دیگر تکثیر شد. تمامی نمونه‌های مثبت گاوی با nested-PCR  اختصاصی از نظر وجود آناپلاسما بوویس بررسی شدند. هم چنین از DNA استخراجی نمونه‌های مثبت، با استفاده از جفت پرایمرهای اختصاصی که قطعه 866 جفت بازی از ژن msp4  آناپلاسما مارژیناله را تکثیر می‌کرد، تکثیر شد. در مجموع 17 درصد از گاوها به آناپلاسما بوویس و 20 درصد به آناپلاسما مارژیناله آلوده بودند. در ارتباط با تأثیرمتغیرهای فصول مختلف سال، سنین مختلف و نوع دامداری بر میزان آلودگی دام‌ها، اختلاف معنی‌داری مشاهده نگردیده است (0/05<P). از آن جایی‌ که در استان گیلان، شرایط مناسب برای فعالیت اکثر کنه‌های سخت فراهم می‌باشد، بنابراین گونه‌های مختلف آناپلاسما در گاوهای استان گیلان شایع می‌باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Molecular identification of Anaplasma bovis and Anaplasma marginale in cows of Gilan province

نویسندگان [English]

  • Nasrollah Vahedi Nouri
  • Vahid Noaman
  • Ebrahim Rahimabadi
Department of Parasitic Disease Research, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Promotion Organization, Karaj, Iran
چکیده [English]

The genus Anaplasma includes species of gram-negative, intracellular bacteria that can affect human and animal health. Anaplasmosis is of special economic importance in the tropical and subtropical regions of the world. These arthropod-borne bacteria all infect the blood cells of eukaryotic hosts and are located exclusively in the intracytoplasmic vacuoles of the cell. The aim of this study was molecular identification of Anaplasma bovis and Anaplasma marginale species in cows of Gilan province. This study was performed on 200 samples of cow blood collected from different parts of Gilan province during 1397. DNA was first extracted from blood samples, and using nested-PCR method, in the first stage, fragment of 1468 bp of Anaplasma16 S rRNA gene was amplified with a specific primer pair. Then, using the original PCR product, the 345 bp fragment was amplified by another specific primer pair. All positive bovine samples were examined by specific nested-PCR for the presence of Anaplasma bovis. Also from positive DNA extraction samples, using a pair of specific primers that amplified the 866 bp fragment of the Anaplasma marginale msp4 gene, reproduced. In total, 17% of cows were infected with Anaplasma bovis and 20% with Anaplasma marginale. In connection with the effect of variables: Different seasons, different ages and types of livestock on the level of livestock pollution, no significant difference was observed (P>0.05).  Since in Gilan province, suitable conditions are provided for the activity of most hard ticks, therefore, different species of Anaplasma are common in cows of Gilan province.

کلیدواژه‌ها [English]

  • Molecular Identification
  • Anaplasma bovis
  • Anaplasma marginale
  • Gilan

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فصلنامه محیط زیست جانوری
دوره 14، شماره 4
بهمن 1401
صفحه 73-80

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