نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه علوم و فنون دریایی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
2 مرکز تحقیقات بیماری‌های پوست و سالک، گروه انگل‌شناسی و قارچ‌شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
In recent decades, extensive studies have been conducted on the effectiveness of marine aquatic extracts in the treatment of diseases including cancer. Based on the results of various experiments in Southeast Asian countries such as Thailand and Japan on the effect of the extract on the treatment of various diseases, this study is an attempt to investigate the effect of coral extract of Sinularia compressa on human gastric adenocarcinoma (AGS) cells. Different concentrations of coral extract 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 μg/ml were extracted. The AGS cell line was then transferred to the laboratory under sterile conditions. This cell line was cultured in "DMEM f12" medium. The cultured cells were then transferred to plate 96 of the well. The cells were incubated at three different times 18, 24, 48 hours. Then, using MTT and flow cytometers, the effectiveness of the extract on AGS cells was measured. Based on the results, it was found that at concentrations above 15 μg/mg, the cells were isolated from the surface of the plate and suspended in the medium. According to the results of the MTT method at a concentration of 10 μg/ml, more than 84% of the cells were destroyed. Based on the findings, the time factor did not show a significant effect on the results. Factor LC50 showed 50% cell death at a concentration of 7.5 μg/ml Sinolaria coral extract. Due to the high effect of this extract on cancer cell death, the lowest concentration was tested in flow cytometry. The results of this experiment showed that cell death of 43% of cells occurred at a concentration of 5 μg/ml of coral extract, which was consistent with the results of MTT test at this concentration. Cell shape changes were also examined. Based on morphological observations, the affected cells became smaller and lost their epithelial shape. S. compressa coral extract showed a great effect on AGS cell mortality. These findings confirm that the potential of using bioactive compounds extracted from marine aquatic animals to control cancer is very significant and can be considered as an alternative to chemical treatments and high side effects.
کلیدواژه‌ها [English]
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