تاثیر منابع مختلف سلنیوم بر فراسنجه‌های کیفی اسپرم خروس های مادر گوشتی مسن

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی، دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

3 گروه علوم دامی، دانشکده کشاورزی، دانشگاه تهران، کرج، ایران

4 گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی گلستان، گرگان، ایران

10.22034/aej.2023.397488.2975

چکیده

یکی از روش های بهبود در باروری، مکمل‌سازی عناصری نظیر سلنیوم به جیره است. اثرات مکمل‌سازی سلنیوم متفاوت است و شدیداً تحت تأثیر منبع و مقدار سلنیوم جیره قرار می گیرد لذا این پژوهش به منظور تاثیر منابع مختلف سلنیوم بر فراسنجه‌های کیفی اسپرم خروس های مادر گوشتی مسن انجام شد. این پژوهش با استفاده از 120 قطعه خروس مادر گوشتی راس 308 با سن 45 هفته در قالب طرح کاملاً تصادفی با چند نمونه در هر واحد آزمایشی 10 تیمار با 4 تکرار و 3 پرنده در داخل هر تکرار اجرا شد. سه منبع مختلف سلنیوم شامل سلنیت سدیم، سلنیوم آلی و نانو بایوکیلات سلنیوم، هر کدام در سه سطح صفر، 0/15، 0/30 و 0/45 میلی‌گرم در کیلوگرم به جیره افزوده شدند. بعد از دو هفته تغذیه با جیره پایه، خروس‌ها به مدت 40 روز و از ابتدای سن 47 هفتگی تیمارهای آزمایشی را دریافت کردند. هر 10 روز یکبار اسپرم‌گیری به روش مالش شکمی از خروس‌ها صورت گرفت سپس فراسنجه‌های کیفی اسپرم خروس ها شامل حجم منی، غلظت، درصد جنبایی، درصد زنده‌مانی، درصد ناهنجاری و درصد عملکرد غشای پلاسمایی اسپرم در زمان های مختلف اندازه گیری شد. تجزیه و تحلیل داده‌ها با استفاده از رویه‌ GLM و نرم افزار SAS 9.4 انجام شد. در مطالعه حاضر، افزودن سلنیوم به جیره در سطح 0/30 میلی‌گرم در کیلوگرم، صرف نظر از منبع آن، موجب افزایش غلظت، درصد جنبایی، زنده‌مانی و عملکرد غشای پلاسمایی اسپرم و کاهش درصد ناهنجاری اسپرم شد. در این مطالعه تغییری در حجم منی با افزودن سلنیوم به جیره خروس‌های مسن مشاهده نشد همچنین غلظت، جنبانی، زندمانی و عملکرد غشای پلاسمایی اسپرم، در روز 40 آزمایش در خروس‌های دریافت کننده 0/30 میلی‌گرم در کیلوگرم سلنیوم از منابع سلنیوم آلی و نانو بایوکیلات سلنیوم به‌طور معنی‌داری بیش تر از سایر زمان ها بود. نتایج نشان داد که شکل آلی سلنیوم (منابع سلمکس و نانوبایوکیلات سلنیوم) نسبت به شکل معدنی (سلنیت سدیم)، در سطح 0/30 میلی‌گرم در کیلوگرم به جیره باعث بهبود عملکرد تولیدمثلی خروس‌های مسن می شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effects of different selenium sources on sperm quality parameters in aged broiler breeder roosters

نویسندگان [English]

  • Morteza Asghari Moghadam 1
  • Seyed Reza Hashemi 1
  • Mehran Mehri 2
  • Amir Karamzadeh Dehaghani 3
  • Homa Davoodi 4
1 Department of Animal Science, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Department of Animal Science, Faculty of Agricalture, Zabol University, Zabol, Iran
3 Department of Animal Science, Faculty of Agricalture, University of Tehran, Karaj, Iran
4 Department of Immunology, Faculty of Medical Science, Golestan University of Medical Science, Gorgan, Iran
چکیده [English]

In order to improve fertility, selenium supplementation serves as an important consideration in animals' diets. The impacts of selenium supplementation depend on the source and quantity of selenium in the diet, so this study was performed to evaluate the effects of different selenium sources on sperm quality parameters in aged broiler breeder roosters (BBR). The study took place over a 40-day period; for the first two weeks, all birds received the same basal diet, and then for the next 40 days, the roosters were fed the experimental diets containing different sources (i.e., sodium selenite, organic selenium, and selenium nano-bio-chelate) and levels of selenium (i.e., 0, 0.15, 0.30, and 0.45 mg/kg). Every ten days, sperm was collected from the BBR by the belly rub method. The collected sperm was evaluated for several qualitative parameters such as volume, concentration, viability, and plasma membrane function. Each parameter was assessed using the General Linear Model (GLM) procedure of SAS 9.4 software. The results of the present study showed that supplementing the diet with selenium at a level of 0.30 mg/kg, regardless of its source, led to an increase in the concentration and motility percentage of sperm, as well as a decrease in the percentage of abnormal sperm. Furthermore, the plasma membrane function of sperm was also enhanced following the addition of selenium to the diet. These findings suggest that selenium supplementation, particularly at a level of 0.30 mg/kg, may improve sperm quality parameters and promote fertility in aged BBR. The present study indicated that the dietary supplementation of selenium, particularly at a level of 0.30 mg/kg, has a positive impact on the reproductive performance of old roosters. The results revealed that the organic form of selenium, such as Selmax and selenium nano-bio-chelate were more effective than sodium selenite, a popular inorganic form of selenium, in enhancing the fertility of the roosters.

کلیدواژه‌ها [English]

  • Rooster
  • nano-bio
  • selenium
  • sperm
  • fertility

مراجع

  1. Ahsan, U., Kamran, Z., Raza, I., Ahmad, S., Babar, W., Riaz, M.H. and Iqbal, Z., 2014. Role of selenium in male reproduction. A review. Animal Reproduction Science. 146: 55-62.
  2. Akhlaghi, A., Ahangari, Y.J., Navidshad, B., Pirsaraei, Z.A., Zhandi, M., Deldar, H., Rezvani, M.R., Dadpasand, M., Hashemi, S.R. and Poureslami, R., 2014. Improvements in semen quality, sperm fatty acids, and reproductive performance in aged Cobb 500 breeder roosters fed diets containing dried ginger rhizomes (Zingiber officinale). Poultry Science. 93: 1236-1244.
  3. Alavi, M.H., Allymehr, M., Talebi, A. and Najafi, G., 2020. Comparative effects of nano-selenium and sodium selenite supplementations on fertility in aged broiler breeder males. Veterinary Research Forum. 11: 135-141.
  4. AlKaabi, A.A.H. and Ali, E.A., 2021. Effect of Dosing of Broiler Breeder Roosters (Ross) with different Levels of Nano-selenium Particles and Organic Selenium on Reproductive traits A Thesis Submitted. Annals of the Romanian Society for Cell Biology. 25: 3718-3726.
  5. AlKaabi, A.A.H. and Ali, E.A., 2021. Effect of Dosing ofBroiler Breeder Roosters (Ross) with different Levels of Nano-selenium Particles and Organic Selenium on Physiological and Histological Traits A Thesis Submitted. Annals of the Romanian Society for Cell Biology. 25: 3859-3870.
  6. Amini, M.R., Kohram, H., Zare Shahaneh, A., Zhandi, M., Sharideh, H. and Nabi, M.M., 2015. The effects of different levels of vitamin E and vitamin C in modified Beltsville extender on rooster post-thawed sperm quality. Cell and Tissue Banking. 16: 587-592.
  7. Ansari, M., Zhandi, M., Kohram, H., Zaghari, M., Sadeghi, M., Gholami, M., Deldar, H., Di Fiore, M.M. and Benson, A.P., 2018. d-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology. Reproduction, Fertility and Development. 30: 1038-1048.
  8. Ashraf, S., Bhatti, S.A., Nawaz, H. and Khan, M.S., 2020. Assessment of Dietary Selenium Sources in Commercial Male Broiler Breeders: Effects on Semen Quality, Antioxidant Status and Immune Responses. Pakistan Veterinary Journal. 40.
  9. Bălăceanu, R.A., Nimigean, V.G., Nimigean, V.R.E.S., Raită, Ş., Ognean, L. and Dojană, N., 2022. Vitamin E and Selenium Given as Dietary Supplements Accumulate in Tissues and Semen and Improve Reproductive Parameters in Older Red Cornish. The Journal of Poultry Science. 59: 96-103.
  10. Blanco, J.M., Wildt, D.E., Höfle, U., Voelker, W. and Donoghue, A.M., 2009. Implementing artificial insemination as an effective tool for ex situ conservation of endangered avian species. Theriogenology. 71: 200-213.
  11. Bréque, C., Surai, P. and Brillard, J.P., 2003. Roles of antioxidants on prolonged storage of avian spermatozoa in vivo and in vitro. Molecular Reproduction and Development: Incorporating Gamete Research. 66: 314-323.
  12. Chauychu-Noo, N., Thananurak, P., Boonkum, W., Vongpralub, T. and Chankitisakul, V., 2021. Effect of organic selenium dietary supplementation on quality and fertility of cryopreserved chicken sperm. Cryobiology. 98: 57-62.
  13. Dalgaard, T.S., Briens, M., Engberg, R.M. and Lauridsen, C., 2018. The influence of selenium and selenoproteins on immune responses of poultry and pigs. Animal Feed Science and Technology. 238: 73-83.
  14. Dalia, A.M., Loh, T.C., Sazili, A.Q., Jahromi, M.F. and Samsudin, A.A., 2018. Effects of vitamin E, inorganic selenium, bacterial organic selenium, and their combinations on immunity response in broiler chickens. BMC Veterinary Research. 14: 1-10.
  15. Ebeid, T.A., 2009. Organic selenium enhances the antioxidative status and quality of cockerel semen under high ambient temperature. British Poultry Science. 50: 641-647.
  16. Edens, F.W. and Sefton, A.E., 2009. Sel-Plex® improves spermatozoa morphology in broiler breeder males. International Journal of Poultry Science. 8: 853-861.
  17. Hama, K.O., 2015. Effect of organic and inorganic sources of selenium on semen quality in roosters. Research Opinions in Animal and Veterinary Sciences. 5: 392-394.
  18. Hezarjaribi, A., Rezaeipour, V. and Abdollahpour, R., 2016. Effects of intramuscular injections of vitamin
    E-selenium and a gonadotropin releasing hormone analogue (GnRHa) on reproductive performance and blood metabolites of post-molt male broiler breeders. Asian Pacific Journal of Reproduction. 5: 156-160.
  19. Huang, Y., Li, W., Xu, D., Li, B., Tian, Y. and Zan, L., 2016. Effect of dietary selenium deficiency on the cell apoptosis and the level of thyroid hormones in chicken. Biological Trace Element Research. 171: 445-452.
  20. Jafari Ahangari, Y., Parizadian, B. and Zamani, M., 2013. The impact of organic selenium supplementation on rooster semen quality in liquid condition. Poultry Science 1: 23-31.
  21. Jones, C.A., Edens, F.W. and Denbow, D.M., 1983. Influence of age on the temperature response of chickens to Escherichia coli and Salmonella typhimurium endotoxins. Poultry Science. 62: 1553-1558.
  22. Khalid, A., Khudhair, N., He, H., Peng, Z., Yaguang, T. and Guixue, Z., 2016. Effects of dietary selenium supplementation on seminiferous tubules and SelW, GPx4, LHCGR, and ACE expression in chicken testis. Biological Trace Element Research. 173: 202-209.
  23. Karimi, A., Besharati, M. and Nemati, Z., 2018.Protective effects of fennel (Foeniculum vulgare) extract on frozen-thowed sperm of Ghezel ram. Journal of Animal Environment. 10(4): 83-90. (In Persian) 
  24. Khalil-Khalili, A.A., Zhandi, M., Zaghari, M., Mehrabani-Yeganeh, H., Yousefi, A.R. and Tavakoli-Alamooti, M., 2021. The effect of dietary organic selenium on reproductive performance of broiler breeder roosters under dexamethasone induced stress. Theriogenology 161: 16-25.
  25. Łukaszewicz, E., Jerysz, A., Partyka, A. and Siudzińska, A., 2008. Efficacy of evaluation of rooster sperm morphology using different staining methods. Research in Veterinary Science. 85: 583-588.
  26. Přinosilová, P., Kopecká, V., Hlavicová, J. and Kunetková, M., 2014. Modified hypoosmotic swelling test for the assessment of boar and bull sperm sensitivity to cryopreservation. Acta Veterinaria Brno. 83: 313-319.
  27. Qazi, I.H., Angel, C., Yang, H., Zoidis, E., Pan, B., Wu, Z., Ming, Z., Zeng, C.-J., Meng, Q. and Han, H., 2019. Role of selenium and selenoproteins in male reproductive function: a review of past and present evidences. Antioxidants. 8: 268.
  28. Raei, H., Torshizi, M.A., Sharafi, M. and Ahmadi, H., 2021. Improving seminal quality and reproductive performance in male broiler breeder by supplementation of camphor. Theriogenology. 166: 1-8.
  29. Rui, B.R., Shibuya, F.Y., Kawaoku, A.J.T., Losano, J.D.A., Angrimani, D.S.R., Dalmazzo, A., Nichi, M. and Pereira, R.J.G., 2017. Impact of induced levels of specific free radicals and malondialdehyde on chicken semen quality and fertility. Theriogenology. 90: 11-19.
  30. Sabzian-Melei, R., Zare-Shahneh, A., Zhandi, M., Yousefi, A.R. and Rafieian-Naeini, H.R., 2022. Effects of dietary supplementation of different sources and levels of selenium on the semen quality and reproductive performance in aged broiler breeder roosters. Poultry Science. 101908.
  31. Santiago-Moreno, J., Castaño, C., Coloma, M.A., Gómez-Brunet, A., Toledano-Díaz, A., López-Sebastián, A. and Campo, J.L., 2009. Use of the hypo-osmotic swelling test and aniline blue staining to improve the evaluation of seasonal sperm variation in native Spanish free-range poultry. Poultry Science. 88: 2661-2669.
  32. Shamiah, S.M., El-Karim, A., Ragaa, E., Eshera, A.A.M., Fouda, F. and Zaghloul, H.K., 2017. Effects of Dietary Selenomethionine Supplementation on Semen Quality, Fertility and Antioxidat Status of Cockerels. Egyptian Journal of Nutrition and Feeds. 20: 227-236.
  33. Sharpe, R.M., McKinnell, C., Kivlin, C. and Fisher, J.S., 2003. Proliferation and functional maturation of Sertoli cells, and their relevance to disorders of testis function in adulthood. Reproduction. 125: 769-784.
  34. Shi, L., Zhang, C., Yue, W., Shi, L., Zhu, X. and Lei, F., 2010. Short-term effect of dietary selenium-enriched yeast on semen parameters, antioxidant status and Se concentration in goat seminal plasma. Animal Feed Science and Technology. 157: 104-108.
  35. Słowińska, M., Jankowski, J., Dietrich, G.J., Karol, H., Liszewska, E., Glogowski, J., Kozłowski, K., Sartowska, K. and Ciereszko, A., 2011. Effect of organic and inorganic forms of selenium in diets on turkey semen quality. Poultry Science. 90: 181-190.
  36. Vahedi, V. and Hedayat Evrigh, N., 2019.Improvement in frozen-thawed ram sperm quality parameters by adding Mentha piperita extract in extender. Journal of Animal Environment. 11(1): 83-90. (In Persian).
  37. Watanabe, T. and Endo, A., 1991. Effects of selenium deficiency on sperm morphology and spermatocyte chromosomes in mice. Mutation Research Letters. 262: 93-99.
فصلنامه محیط زیست جانوری
دوره 15، شماره 2
مرداد 1402
صفحه 149-156

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