مقایسه خواص آنتی اکسیدانی بین عصاره‌ سه گونه جلبک بومی سبز و قهوه‌ای در سواحل شمالی دریای عمان

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه شیلات، واحد چابهار، دانشگاه آزاد اسلامی، چابهار، ایران

10.22034/aej.2022.333075.2772

چکیده

حضور ماکروجلبک ‌ها در زیستگاه‌ های آبی بسیار حائز اهمیت می‌باشد. این گیاهان امروزه به عنوان منابع غنی از آنتی‌ اکسیدان ‌های طبیعی مورد توجه بسیاری از محققین قرار گرفته ‌اند. آنها اثرات سودمند متعددی روی فعالیتهای بیولوژیکی مانند آنتی اکسیدان ها و سمیت سلولی از خود نشان داده اند. در همین ارتباط در پژوهش حاضر خواص آنتی اکسیدانی سه گونه از ماکرو جلبک‌ های بومی در سواحل شمالی دریای عمان (استان سیستان و بلوچستان) بررسی و مقایسه شد. نمونه برداری در فصول پاییز و زمستان 1397 از سواحل شمالی دریای عمان (خلیج چابهار) انجام شد. نمونه‌ های مورد مطالعه شامل یک گونه جلبک سبز (Ulva lactuca) و دو گونه جلبک قهوه‌ای (Sargassum illicifolium و Nizamuddinia zanardinii) بود. این گونه‌ها به شکل غالب در سواحل شمالی دریای عمان حضور دارند. بعد از نمونه برداری جلبک ها توسط آب دریا و سپس آب شیرین شستشو شدند.سپس نمونه های تهیه شده خشک و سپس آسیاب گردیدند. تهیه عصاره در سه مرحله انجام شد. مراحل اول و دوم توسط آب مقطر و مرحله سوم توسط حلال متانول بود. سنجش میزان آنتوسیانین و محتوای فلاونوئیدی به روش اسپکتوفتومتری انجام شد. خواص آنتی‌ اکسیدانی نیز با اندازه‌ گیری قدرت مهارکنندگی یون آهن اندازه‌ گیری گردید. بر اساس نتایج پژوهش، اختلاف معنی‌ داری در شاخص‌ های اندازه‌گیری شده بین گونه‌های مورد مطالعه مشاهده شد (0/05>p). میزان آنتوسیانین تام در عصاره S. illicifoliu، N. zanardinii و U. lactuca به ترتیب، 1/14±8/31، 1/36±5/19 و 1/84±3/59 میلی‌گرم در 100 گرم وزن خشک اندازه گیری شد (0/05>p). هم چنین محتوای فلاونوئیدی تام نیز بین عصاره های مورد مطالعه به ترتیب 2/71±27/40، 1/14±21/12 و 0/12±0/39 میلی‌گرم کوئرستین بر 100 گرم وزن خشک و قدرت مهارکنندگی یون آهن به ترتیب 0/36±1/21، 0/10±0/67 و 0/12±0/60 بود (0/05>p). در مجموع نتایج حاضر نشان داد که میزان آنتوسیانین، محتوای فلاونوئیدی و قدرت مهارکنندگی یون آهن در عصاره S. illicifolium به شکل معنی‌داری بالاتر از عصاره‌های U. lactuca و N. zanardinii می‌باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Comparison of antioxidant properties between extracts of three species of green and brown algae on the northern shores of the Oman Sea

نویسندگان [English]

  • Ali Mahdi Abkenar
  • Eid Mohammad Sarani Yaztapeh
Department of Fisheries, Chabahar Branch, Islamic Azad University, Chabahar, Iran
چکیده [English]

The presence of macroalgae, are important in aquatic habitats. Nowadays, this plants have been receiving increasing attention by many researchers as a promising natural rich sources of antioxidants. They have displayed numerous advantageous effects on biological activities, including antioxidants and cytotoxicity. In this regard, at the present study, the antioxidant properties of three species of native macroalgae to the northern coast of the Oman Sea (Sistan and Baluchestan province) were studied and compared. Sampling was carried out in autumn and winter of 2018 on the northern coast of the Oman Sea (Chabahar Gulf). The samples included one species of green (Ulva lactuca) and two brown (Sargassum illicifolium and Nizamuddinia zanardinii) algae. These species are predominantly found on the northern shores of the Oman Sea. After sampling, the algae were washed with sea water and tap water. The prepared samples were then dried and powdered with an electric mill. The extract was prepared in three steps. The first and second stages was carried out with distilled water and the third stage with methanol solvent. The total content of anthocyanin and flavonoid were measured by spectrophotometry method. Antioxidant properties were also measured by ferric reducing antioxidant power. Based on the results, a significant difference were observed in the studied indices between the studied species (p<0.05). Total anthocyanin contents in S. illicifolium, N. zanardinii and U. lactuca extracts was 8.31±1.14, 5.19±1.36 and 3.59±1.84 mg per 100 g dry weight, respectively (p<0.05). However, total flavonoid content between the studied extracts was 27.40±2.71, 21.12±1.14 and 0.39±0.12 mg per 100 g QE per 100 g dry weight and ferric reducing antioxidant power was 1.21±0.36, 0.67±0.10 and 0.60±0.12, respectively (p<0.05). In conclusion, the results of this study indicate that the total amount of anthocyanin and flavonoid contents as well as ferric reducing antioxidant power in S. illicifolium extract were significantly higher than U. lactuca and N. zanardinii extracts.

کلیدواژه‌ها [English]

  • Antioxidants
  • anthocyanin
  • flavonoid
  • Oman Sea
  • FRAP
  • Sargassum illicifolium

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فصلنامه محیط زیست جانوری
دوره 15، شماره 1
خرداد 1402
صفحه 295-302

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