تاثیر مقادیر مختلف پودر افشره طبیعی پوست انار و یک پری بیوتیک تجاری بر عملکرد رشد و جمعیت میکروبی دستگاه‌ گوارش جوجه های گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تغذیه دام و طیور، دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 گروه علوم دامی، دانشکده پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

چکیده

این پژوهش به منظور مقایسه تاثیر مقادیر مختلف پودر افشره طبیعی پوست انار با یک پری‌بیوتیک تجاری بر عملکرد رشد و جمعیت میکروبی دستگاه گوارش جوجه‌های‌گوشتی انجام شد. این آزمایش با 250 قطعه جوجه نر یک روزه سویه راس 308 در 5 تیمار و 5 تکرار در قالب طرح کاملا تصادفی به مدت 42 روز انجام شد. تیمارهای آزمایشی شامل مقادیر صفر(گروه شاهد)، 150، 300 و 450 میلی‌گرم پودر افشره طبیعی پوست انار در هر کیلو‌گرم و یک تیمار حاوی 1000 میلی‌گرم پری‌بیوتیک در هر کیلوگرم خوراک بودند. عملکرد رشد پرنده در سه دوره آغازین، رشد و پایانی ثبت و جمعیت میکروبی لاکتوباسیل‌ها، کلی‌فرم و کل باکتری‌های هوازی و pH ایلئوم و سکوم آنها در سن 42 روزگی اندازه‌‌گیری شد. نتایج این پژوهش نشان داد که افزودن 150 و 300 میلی‌گرم پودر افشره طبیعی پوست انار وزن زنده در سن 42 روزگی را نسبت به سایر تیمارها افزایش داد (0/05˂P)، اما تاثیری بر خوراک مصرفی و ضریب تبدیل غذایی در سن 42 روزگی نداشت. استفاده از پری‌بیوتیک نسبت به گروه شاهد نه تنها تاثیری بر عملکرد رشد در کل دوره پرورش (1 تا 42 روزگی) نداشت بلکه باعث افزایش جمعیت کلی‌فرم‌ها در ناحیه ایلئوم در سن 42 روزگی شد (0/05˂P). افزودن 150 میلی‌گرم پودر افشره طبیعی پوست انار در مقایسه با سایر تیمارها منجر به افزایش جمعیت لاکتوباسیل‌ها در ناحیه ایلئوم شد (0/05˂P). هم‌چنین، تاثیر تیمارهای آزمایشی بر پی‌اچ ایلئوم و سکوم در سن 42 روزگی معنی‌دار نبود. بنابراین، استفاده از 150 میلی‌گرم پودر افشره طبیعی پوست انار در هر کیلوگرم جیره به عنوان افزودنی خوراکی تاثیر مثبتی بر عملکرد رشد و جمعیت میکروبی لاکتوباسیلوس‌های دستگاه گوارش جوجه‌های گوشتی داشت.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effects of different levels of pomegranate peel crude juice powder and a commercial prebiotic on growth performance and gut microbial population of boilers chickens

نویسندگان [English]

  • Seyed Ghasem Shirangi 1
  • Mahmoud Shams Shargh 1
  • Omid Ashayerzadeh 1
  • Mohammad Hossein Mohammadi Ghasem Abadi 2
1 Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

This study was conducted to compare the effects of different levels of pomegranate peel crude juice powder (PPCJP) with a commercial prebiotic on growth performance and gut microbial population of boiler chicks. This trial was done with 250 one-day-old Ross 308 male chicks in five treatments and five replicates in a completely randomized design for 42 days. The treatments included zero (control), 150, 300 and 450 mg of PPCJP per kg feed and 1000 mg commercial prebiotic per kg feed. The growth performance parameters of broilers in starter, grower and finisher periods were recorded and microbial population of Lactobacillus spp., Coliforms spp. and Total aerobics bacteria spp. of ileum and ceca were measured at day 42. Although adding 150 and 300 mg of PPCJP in comparison with other treatments increased BW at day 42 (P<0.05), these treatments had no significant effects on feed intake and feed conversion ratio at day 42. Not only adding prebiotic in contrast with control group had no significant effects on the whole period (1 until 42 d) growth performance, but also increased ileum Coliforms spp. population at day 42 (P<0.05). Adding 150 mg of PPCJP significantly increased (P<0.05) ileum Lactobacillus spp. population compared with other treatments. Moreover, the effect of treatments on ileum and ceca were not significant at day 42. Overall, supplementing 150 mg PPCJP per kg of diet as a feed additive had the positive effects on growth performance and gut microbial population of broiler chicks.

کلیدواژه‌ها [English]

  • Broiler
  • Microbial population
  • Prebiotic
  • Performance
  • Pomegranate peel crude juice

مراجع

  1. Bahrekazemi, M., Memarian, K. and Nikbakhsh, J., 2020. Effect of continuous and intermittent oral administration of prebiotic mito on the growth factors, body composition, and hematological parameters in common Carp Cyprinus carpio. Journal of Animal Environment. 11(4): 229-236. (In Persian)
  2. Shams Shargh, M. and Khosravi, A., 2011. Feed additives in poultry nutrition. Gorgan University of Agricultural Sciences and Natural Resources. Nourozi Publications, first edition. 208 p. (In Persian)
  3. Saleh, H., Golian, A., Kermanshahi, H., Farhoosh, R. and Taher Mirakzehi, M., 2015. Effect of α-Tocopherol Acetate, Pomogrante Peel and Extract on Performance, Nutrient Digestibilities and Tibia Bone Calcifaction in Broiler Chickens. Research on Animal Production. 6(12): 1-9. (In Persian)
  4. Naraghi Rad, Z., Ghoreishi, S.M. and Kargar, Sh., 2021. Chemical Composition and In Vitro Digestibility of Pomegranate Byproducts in Ruminant diet. Journal of Animal Environment. 13(2): 67-74. (In Persian)
  5. Adhikari, P.A. and Kim, W.K., 2017. Overview of prebiotics and Probiotics: Focus on Performance, Gut health and immunity. Animal Science. 17: 949-966.
  6. Ahmed, S.T., Islam, M.M., Bostami, A.R., Mun, H.S., Kim, Y.J. and Yang, C.J., 2015. Meat composition, fatty acid profile and oxidative stability of meat from broilers supplemented with pomegranate (Punica granatum) by-products. Food Chemistry. 188: 481-488.
  7. Akuru, E.A., Mpendulo, C.T., Oyeagu, C.E. and Nantapo, C.W.T., 2021. Pomegranate (Punica granatum) peel powder meal supplementation in broilers: effect on growth performance, digestibility, carcase and organ weights, serum and some meat antioxidant enzyme biomarkers. Italian Journal of Animal Science. 20(1): 119-131.
  8. Akuru, E.A., Oyeagu, C.E., Mpendulo, T.C., Rautenbach, F. and Oguntibeju, O.O., 2020. Effect of pomegranate (Punica granatum L) peel powder meal dietary supplementation on antioxidant status and quality of breast meat in broilers. Heliyon. 6(12): 1-12.
  9. Al-Khalaifa, H., Al-Nasser, A., Al-Surayee, T., Al-Kandari, S., Al-Enzi, N., Al-Sharrah, T., Ragheb, G., Al-Qalaf, S. and Mohammed, A., 2019. Effect of dietary probiotics and prebiotics on the performance of broiler chickens. Poultry Science. 98(10): 4465-4479.
  10. Bozkurt, M., Küçükyilmaz, K., Çatli, A.U. and Çinar, M., 2008. Growth performance and slaughter characteristics of broiler chickens fed with antibiotic, mannan oligosaccharide and dextran oligosaccharide supplemented diets. International Journal of Poultry Science. 7(10): 969-977.
  11. Bostami, A.B.M., Ahmed, S.T., Islam, M.M., Mun, H.S., Ko, S.Y., Kim, S.S. and Yang, C.J., 2015. Growth performance, fecal noxious gas emission and economic efficacy in broilers fed fermented pomegranate byproducts as residue of fruit industry. International Journal Advance. 3: 102-114.
  12. Brenes, A., Viveros, A., Goñí, I., Centeno, C., Sayago-Ayerdy, S., Arija, I. and Saura-Calixto, F., 2008. Effect of grape pomace concentrate and vitamin E on digestibility of polyphenols and antioxidant activity in chickens. Poultry Science. 87: 307-316.
  13. Celik, I., Temur, A. and Isik, I., 2009. Hepato protective role and antioxidant capacity pomegranate flowers infusion against trichloroacetic acid-exposed in rats. Food and Chemical Toxicology. 47: 145-149.
  14. Choi, J. and Kim, W.K., 2020. Dietary Application of Tannins as a Potential Mitigation Strategy for Current Challenges in Poultry Production: A Review. Animals. 10(12): 1-21.
  15. Dikmen, M., Ozturk, N. and Ozturk, Y., 2011. The antioxidant potency of Punica granatum L. fruit peel reduces cell proliferation and induces apoptosis on breast cancer. Journal of Medicinal Food. 14: 219-228.
  16. Farag, R.S., Arafat, S.M. and Tawfeek, L.S., 2015. Antioxidant activities of pomegranate peels and leave crude juices on sunflower oil oxidative rancidity. Journal of Medical and Biological Science Research.1(7): 92-99.
  17. Gibson, G.R. and Roberfroid, M.B., 1995. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. The Journal of Nutrition. 125(6): 1401-1412.
  18. Gibson, G.R., Probert, H.M., Van Loo, J., Rastall, R.A. and Roberfroid, M.B., 2004. Dietary modulation of the human colonic microbiota: updating the concept of prebiotics. Nutrition Research Reviews. 17(2): 259-275.
  19. Gopi, M., Dutta, N., Pattanaik, A.K., Jadhav, S.E. and Mohan, J., 2017. Diet composition and polyphenols improved performance in heat stress broilers. In the 28th Annual Australian Poultry Science Symposium. 252-255.
  20. Hamady, G.A.A., Abdel-Moneim, M.A., El-Chaghaby, G.A., Abd-El-Ghany, Z.M. and Hassanin, M.S., 2015. Effect of Pomegranate peel extract as natural growth promoter on the productive performance and intestinal microbiota of broiler chickens. African Journal Science Technology. 3: 514-519.
  21. Hashemi, S.R. and Davoodi, H., 2011. Herbal plants and their derivatives as growth and health promoters in animal nutrition. Veterinary Research Communications. 35: 169-180.
  22. Hassan, Z.M., Manyelo, T.G., Selaledi, L. and Mabelebele, M., 2020. The Effects of Tannins in Monogastric Animals with Special Reference to Alternative Feed Ingredients. Molecules. 25(20): 1-17.
  23. Houshmand, M., Azhar, K., Zulkifli, I., Bejo, M.H. and Kamyab, A., 2012. Effects of prebiotic, protein level, and stocking density on performance, immunity, and stress indicators of broilers. Poultry Science. 91(2): 393-401.
  24. Ibrahim, A., Awad, S. and El-Sayed, M., 2020. Impact of pomegranate peel as prebiotic in bio-yoghurt. British Food Journal. 122(9): 2911-2926.
  25. Kishawy, A.T.K., Amer, S.A., El-Hack, M.A., Saadeldin, I.M. and Swelum, A.A., 2019. The impact of dietary linseed oil and pomegranate peel extract on broiler growth, carcass traits, serum lipid profile, and meat fatty acid, phenol, and flavonoid contents. Asian-Australians Journal Animal Science. 32: 1161-1171.
  26. Lavina, S., Gabi, D., Drinceano, D., Stef, D., Daniela, M., Julean. C., Ramona, T. and Corcionivoschi, N., 2009. The effect of medicinal plants and plant extracted oils on broiler duodenum morphology and immunological profile. Journal of Romanian Society of Biological Sciences. 14: 4606-4614.
  27. Mabelebele, M., Norris, D., Brown, D., Ginindza, M.M. and Ngambi, J.W., 2017. Breed and sex differences in the gross anatomy, digesta pH and histomorphology of the gastrointestinal tract of gallus gallus domesticus. Brazilian Journal of Poultry Science.19: 339-346.
  28. Mahmoudi, M., Farhoomand, P. and Nourmohammadi, R., 2015. Effects of different levels of hemp seed (Cannabis sativa) and dextran oligosaccharide on growth performance and antibody titer response of broiler chickens. Italian Journal of Animal Science. 14(1): 114-119.
  29. Magangana, T.P., Makunga, N.P., Fawole, O.A. and Opara, U.L., 2020. Processing Factors Affecting the Phytochemical and Nutritional Properties of Pomegranate (Punica granatum) Peel Waste: A Review. Molecules. 25(20): 4690.
  30. Mašek, T., Starčević, K. and Mikulec, Ž., 2014. The influence of the addition of thymol, tannic acid or gallic acid to broiler diet on growth performance, serum malondialdehyde value and cecal fermentation. European Poultry Science. 78: 1-8.
  31. Mathew, A.G., Cissell, R. and Liamthong, S., 2007. Antibiotic resistance in bacteria associated with food animals: a United States perspective of livestock production. Foodborne pathogens and disease. 4(2): 115-133.
  32. Mohammadi Ghasem Abadi, M.H., Moravej, H., Shivazad, M., Torshizi, M.A.K. and Kim, W.K., 2019. Effects of feed form and particle size, and pellet binder on performance, digestive tract parameters, intestinal morphology, and cecal microflora populations in broilers. Poultry Science. 98(3): 1432-1440.
  33. Oduho, G.W. and Baker, D.H., 2005. Some tropical high tannin sorghums and their effects on broiler performance. Agricultura Tropica et Subtropica. 38(3-4): 105-111.
  34. Orgil, O. and Amir, R., 2014. The antioxidative and antiproliferative potential of non-edible organs of the pomegranate fruit and tree. Food Science and Technology. 58: 571-577.
  35. Perricone, V., Comi, M., Giromini, C., Rebucci, R., Agazzi, A., Savoini, G. and Bontempo, V., 2020. Green Tea and Pomegranate Extract Administered During Critical Moments of the Production Cycle Improves Blood Antiradical Activity and Alters Cecal Microbial Ecology of Broiler Chickens. Journal of Animals. 8: 785-798.
  36. Rao, S.R., Raju, M.V.L.N., Prakash, B., Rajkumar, U. and Reddy, E.P.K., 2019. Effect of supplementing moringa (Moringa oleifera) leaf meal and pomegranate (Punica granatum) peel meal on performance, carcass attributes, immune and antioxidant responses in broiler chickens. Animal Production Science. 59(2): 288-294.
  37. Rehman, A., Arif, M., Sajjad, N., Al-Ghadi, M.Q., Alagawany, M., Abd El-Hack, M.E., Alhimaidi, A.R., Elnesr, S.S., Almutairi, B.O., Amran, R.A. and Hussein, E.O.S., 2020. Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity. Poultry Science. 99(12): 6946-6953.
  38. Rajani, J., Karimi Torshizi, M.A. and Rahimi, M.S., 2011. Control of Ascites Mortality and Improved Performance and Meat Shelf life in Broilers Using Feed Adjuncts with Presumed Antioxidant Activity. Animal Feed Science and Technology. 170: 239-245.
  39. Salehi, M., Hosseini, S.A.E., Rasoulpour, R., Salehi, E. and Bertaccini, A., 2016. Identification of a phytoplasma associated with pomegranate little leaf disease in Iran. Crop Protection. 87: 50-54.
  40. Sarangi, N.R., Babu, L.K., Kumar, A., Pradhan, C.R., Pati, P.K. and Mishra, J.P., 2016. Effect of dietary supplementation of prebiotic, probiotic, and synbiotic on growth performance and carcass characteristics of broiler chickens. Veterinary World. 9(3): 313-319.
  41.  SAS. 2003. SAS/STATE Users Guide: 2003 Edition: SAS Institute Inc., Cary. Nc.
  42. Sharifian, M., Hosseini-vashan, S.J., Fathi-nasri, M.H. and Perai, A.H., 2019. Pomegranate peel extract for broiler chickens under heat stress: Its influence on growth performance, carcass traits, blood metabolites, immunity, jejunal morphology, and meat quality. Livestock Science. 227: 22-28.
  43. Sorrenti, V., Randazzo, C.L., Caggia, C., Ballistreri, G., Romeo, F.V., Fabroni, S., Timpanaro, N., Raffaele, M. and Vanella, L., 2019. Beneficial effects of pomegranate peel extract and probiotics on pre-adipocyte differentiation. Frontiers in Microbiology. 10: 1-11.
  44. Teng, P.Y. and Kim, W.K., Review: Roles of Prebiotics in Intestinal Ecosystem of Broilers. Front Veterinary Science. 1-18.
  45. Tripathi, K., 2004. Essential of medical pharmacology. Jaypee Brothers Medical Publishers. New Dehli. India.
  46. Ullah, N., Ali, J., Khan, F.A., Khurram, M., Hussain, A., Rahman, I.U., Rahman, Z.U. and Ullah, S., 2012. Proximate composition, minerals content, antibacterial and antifungal activity evaluation of pomegranate (Punica granatum) peels powder. Middle-East Journal of Scientific Research. 11(3): 396-401.
فصلنامه محیط زیست جانوری
دوره 15، شماره 2
مرداد 1402
صفحه 169-176

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