تاثیر خوراکی افزودنی لیورگل بر شاخص های رشد و پیشگیری از عارضه کبد چرب ماهی کوی (Cyprinus carpio var. Koi) تغذیه شده با رژیم غذایی حاوی سطوح مختلف چربی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، دانشکده شیلات و محیط زیست، واحد آزادشهر، دانشگاه آزاد اسلامی، آزادشهر، ایران

2 گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

10.22034/aej.2021.282955.2514

چکیده

لیورگل از عصاره گیاهی خار مریم تهیه می شود. خار مریم محتوی فلاوانو لیگنان ها می باشد و دارای اثرات محافظت کننده‌ کبدی است. در این آزمایش به بررسی اثرات خوراکی لیورگل بر شاخص های رشد و پیشگیری از عارضه کبد چرب در سطوح مختلف چربی جیره ماهی کوی پرداخته شد. برای این آزمایش 9 جیره غذایی مختلف با 3 سطح 0، 0/5 و 1 درصد لیورگل با سه سطح 5 ،10 و 20 درصد چربی تهیه گردید. غذادهی به ماهیان به مدت 150 روز انجام شد. پس از پایان دوره پرورش، شاخص های رشد ماهی (وزن انتهایی، افزایش وزن، نرخ رشد ویژه، بازماندگی و ضریب تبدیل غذایی) و نمونه برداری از بافت کبد ماهیان مورد بررسی قرار گرفت. نتایج آزمایش نشان داد وزن نهایی، افزایش وزن بدن، نرخ رشد ویژه و ضریب تبدیل غذایی در میان تیمارهای آزمایشی معنی دار بود (0/05>P)، در حالی که بازماندگی در میان تیمارها تفاوت معنی داری را نشان نداد (0/05<P). بالاترین شاخص های رشد در تیمار 8 (چربی 20 درصد + داروی لیورگل 1 درصد) مشاهده شد. هم چنین نتایج آزمایش نشان داد که در بین تمام تیمارهای آزمایش بیش ترین عارضه کبدی در تیمار 6 که حاوی جیره غذایی 20 درصد چربی و بدون داروی لیورگل بود مشاهده شد. نتیجه نهایی تحقیق نشان داد در تمام تیمارهای چربی جیره غذایی (5، 10 و 20 درصد) اضافه کردن داروی لیورگل به جیره غذایی احتمالا به علت وجود سیلی مارین باعث کاهش ضایعات کبدی در ماهی کوی گردید.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of livergol drug on growth performance and preservation of fatty liver in Koi (Cyprinus carpio var. Koi) fed with diets containing different level of fatty.

نویسندگان [English]

  • Mohadeseh Ahmadi 1
  • Abdolmajid Hajimoradloo 2
1 Department of Fisheries, Faculty of Fisheries and Environment, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran
2 Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

Livergol drug is made from a herbal extract of Mary Magenta. Milk thistle contains flavonoids and has hepatoprotective effects. The purpose of this study was to evaluate the effects of livergol drug on growth indices and prevention of fatty liver disease in different levels of fat in Koi fish diets. For this experiment, 9 diets of different nutrients were prepared with 3 levels of 0, 0.5 and 1% of livergol and three levels of 5, 10 and 20% fat. The fish were fed for 150 days. After the end of breeding period, fish growth indices (end weight, weight gain, specific growth rate, survival rate and food conversion ratio) and samples of fish liver tissue were investigated. The results of the experiment showed that the final weight, body weight gain, specific growth rate and feed conversion ratio were significant in the experimental treatments (P≤0.05). While survival did not show significant difference among treatments (P≥0.05).The highest growth indices were observed in treatment 8 (fat 20% + livergol 1%). The results of the experiment showed that among all treatments, the highest liver toxicity among the all groups was observed in treatment 6 containing 20% fat and without livergol. The final result of the study showed that in all dietary fat treatments (5, 10 and 20%), adding livergol drug to the diet was probably due to the presence of silimarin reduced liver lesions in Koi fish.

کلیدواژه‌ها [English]

  • Koi fish
  • Livergol
  • Growth index
  • Fatty liver
  1. Abdel-Tawwab, M. and Ahmad, M.H., 2009. Effect of dietary protein regime during the growing period on growth performance, feed utilization and whole-body chemical composition of Nile Tilapia, Oreochromis niloticus (L.). Aquaculture Research. 40(13): 1532-1537.
  2. Hall, P.D.E.L.A.M., Plummer, J.L., Ilsley, A.H., Ahern, M.J., Cmielewski, P.L. and Williams, R.A., 2015. The pathology of liver injury induced by the chronic administration of alcohol and ‘low-dose’ carbon tetrachloride in Porton rats. Journal of Gastroenterology and Hepatology. 9(4): 305-317.
  3. Webster, C.D. and Lim, C., 2002. Nutrient requirements and feeding of finfish for aquaculture. Auburn, Alabama, USA: CAB International Publishing.
  4. López, B. and Bureau, D.P., 2015. Effect of dietary lipid levels on performance, body composition and fatty acid profile of juvenile white seabass, Atractoscion nobilis. Aquaculture. 306(9): 297-309.
  5. Haghighi, A., 2007. Pathology of fish and shrimp. Editor: Sohrabi Haqdoost, A., Tehran, Iran: Islamic Azad University, Tehran Science and Research Unit, Faculty of Veterinary Science. 411 p. (In Persian)
  6. Spanò, L., Tyler, C.R., Van Aerle, R., Devos, P., Mandiki, S.N.M., Silvestre, F. and Kestemont, P., 2014. Effects of atrazine on sex steroid dynamics, plasma vitellogenin concentration and gonad development in adult goldfish (Carassius auratus). Aquatic Toxicology. 90(5): 535-543.
  7. Ogata, H.Y. and Oku, H., 2016. The effects of dietary retinoic acid on body lipid deposition in juvenile red sea bream (Pagrus major); a preliminary study. Aquaculture. 298(8): 631-640.
  8. Hasanloo, T., Khavari Nejad, R. and Majidi, E., 2007. Evaluation of Phenotypic Coefficient and Flavonolignan Content in Dried Fruits of Cultivated and Endemic Silybum marianum(L.) Gaertn. Journal of Medicinal Plants. 6(22): 77-90. (In Persian)
  9. Vogel, G., Trost, W., Braatz, R., Odenthal, K.P., Brüsewitz, G., Antweiler, H. and Seeger, R., 2016. Pharmacodynamics, site and mechanism of action of silymarin, the antihepatoxic principle from Silybum mar. (L) Gaertn. 1. Acute toxicology or tolerance, general and specific (liver-) pharmacology. Arzneimittel-Forschung. 86(4): 201-212.
  10. PourAmir, M., Sajadi, P., Shahabi, S., Rezaei, S. and Samadi, P., 2006. Effects of food diet of tomato and carrot juices on serum lipids in rats. Journal of Birjand University of Medical Sciences. 13(2): 9-15. (In Persian)
  11. Škottová, N., Večevra, R., Urbánek, K., Vávna, P., Walterová, D. and Cvak, L., 2003. Effects of polyphenolic fraction of silymarin on lipoprotein profile in rats fed cholesterol-rich diets. Pharmacological Research. 47(1): 17-26.
  12. Muriel, P. and Mourelle, M., 2016. Prevention by silymarin of membrane alterations in acute CCI4 liver damage. Journal of Applied Toxicology. 34(6): 365-373.
  13. Naseri, M., 2016. Maintaining health from the perspective of traditional Iranian medicine. Editor: Parviz, M., Traditional Iranian Medicine. Tehran, Iran. 189 p. (In Persian)
  14. Yang, S.D., Lin, T.S., Liu, F.G. and Liou, C.H., 2007. Influence of dietary phosphorus levels on growth, metabolic response and body composition of juvenile silver perch (Bidyanus bidyanus). Aquaculture. 254(5): 405-413.
  15. Pourmozaffar, S., Hajimoradloo, A., Paknejad, H. and Rameshi, H., 2019. Effect of dietary supplementation with apple cider vinegar and propionic acid on hemolymph chemistry, intestinal microbiota and histological structure of hepatopancreas in white shrimp, Litopenaeus vannamei. Fish and Shellfish Immunology. 86: 900-905. https://doi.org/10.1016/j.fsi.2018.12.019
  16. Azarin, H., Imanpour, M.R., Karamad, A., Jebeleh, A.R. and Pourmozaffar, S., 2020. In vitro oocyte maturation and blood-circulating steroid hormones in Sterlet (Acipenser ruthenus) and Siberian sturgeon (Acipenser baeri). Aquaculture Research. 51(9): 3488-3494. https://doi.org/10.1111/are.14683
  17. Golalipour, Y., Khara, H. and Mohseni, M., 2016. Effects of Different levels of lipid on liver enzymes, blood hematological and biochemical parameters in Siberian sturgeon (Acipenser Bearii). Journal of Animal Environment. 8(1): 229-236. (In Persian)
  18. Neverian, H.A., Mostafazadeh, S. and Toluei, M.H., 2005. A study on various protein levels on growth indices (SR, WG, RGR, FCR and PER) of Rutilus frisii kutum, Kamenskii 1901(Advanced fry) Pajouhesh & Sazandegi. 68: 61-68. (In Persian)
  19. Shymaa, M., Shalaby, A.Y., Olfat, M.W. and Saoud, I.P., 2018. Growth, feed utilization and body composition of white Rutilus rutilus juveniles offered diets with various protein and energy levels. Journal of King Saud University. Science. 27: 12-23.
  20. Schuchardt, D., Vergara, J.M., Fernandez, H., Izquierdo, M.S. and Robaina, L., 2018. Effects of different dietary protein and lipid levels on growth, feed utilization and body composition of Huso huso. Aquaculture Nutrition. 22(3): 57-66.
  21. Li, X., Jiang, Y., Liu, W. and Ge, X., 2012. Protein-sparing effect of dietary lipid in practical diets for blunt snout bream (Megalobrama amblycephala) fingerlings: effects on digestive and metabolic responses. Fish Physiology and Biochemistry. 38(2): 529-541.
  22. Chai, X.J., Ji, W.X., Han, H., Dai, Y.X. and Wang, Y., 2013. Growth, feed utilization, body composition and swimming performance of giant croaker, Nibea japonica Temminck and Schlegel, fed at different dietary protein and lipid levels. Aquaculture Nutrition. 19(6): 928-935.
  23. Du, Z.Y., Liu, Y.J., Tian, L.X., Wang, J.T., Wang, Y. and Liang, G.Y., 2005. Effect of dietary lipid level on growth, feed utilization and body composition by juvenile grass carp (Ctenopharyngodon idella). Aquaculture Nutrition. 11(2): 139-146.
  24. Sadeghi, , Imanpour, M.R., Shabani, A., Mazandarani, M. and Bagheri, T., 2018. Effect of dietary lipid levels on some growth indices and survival rate of gold fish (Carassius auratus). Journal of Animal Environment. 10(1): 231-236. (In Persian)
  25. Ebrahimi, R., Mohammadabadi, T., Sari, M., Salari, S., Zamiri, M.J. and Beigi Nasiri, M.T., 2013. Effect of silymarin against lead induced oxidative stress in broiler chicken. Iranian Journal of Animal Science Research. 5(4): 302-312.
  26. Thyagarajan, S.P., Jayaram, S., Gopalakrishnan, V., Hari, R., Jeyakumar, P. and Sripathi, M.S., 2002. Herbal medicines for liver diseases in India. Journal of Gastroenterology and Hepatology. 17: 370-376.
  27. Windisch, W., Schedle, K., Plitzner, C. and Kroismayr, A., 2018. Use of phytogenic products as feed additives for swine and poultry. Journal of Animal Science. 90(6): 208-217.
  28. Golalipour, Y., Khara, H. and Mohseni, M., 2017. Effects of different levels of lipid on growth and body composition of Siberian sturgeon Acipenser Bearii Brandt, 1869. Journal of Applied Ichthyological Research. 5(3): 91-102. (In Persian)
  29. Hinton, D.E., Lauren, D.J., Holliday, T.L. and Giam, C.S., 2016. Liver structural alterations accompanying chronic toxicity in fishes: potential biomarkers of exposure. Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry. American Chemical Society. 12(6): 404-413.
  30. Lettéron, P., Labbe, G., Degott, C., Berson, A., Fromenty, B., Delaforge, M. and Pessayre, D., 2018. Mechanism for the protective effects of silymarin against carbon tetrachloride-induced lipid peroxidation and hepatotoxicity in mice: evidence that silymarin acts both as an inhibitor of metabolic activation and as a chain-breaking antioxidant. Biochemical Pharmacology. 56(14): 1350-1362.
  31. Sobolová, L., Škottová, N., Veče\vra, R. and Urbánek, K., 2017. Effect of silymarin and its polyphenolic fraction on cholesterol absorption in rats. Pharmacological Research. 64(4): 202-213.
  32. Saller, R., Melzer, J., Reichling, J., Brignoli, R. and Meier, R., 2016. An updated systematic review of the pharmacology of silymarin. Complementary Medicine Research. 18(6): 109-121.