تاثیر شدت‌های مختلف امواج الکترومغناطیس بر فراسنجه‌های اسپرم و شاخص استرس در منی قوچ نژاد لری بختیاری

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

10.22034/aej.2022.362925.2887

چکیده

میدان‌های الکترومغناطیسی و تابش‌ها با فرکانس رادیویی روی بافت‌های بدن انسان برهمکنش داشته و می‌توانند روی تولیدمثل اثراتی داشته باشند و بیش ترین عوارض مطرح شده امواج تأثیر روی سیستم تولیدمثل، آنزیم‌های بدن، هورمون‌های جنسی مردانه، مغز استخوان، ضربان قلب، تغییرات هماتولوژیک و اختلال‌های عصبی- رفتاری است. این تخقیق بررسی تاثیر شدت امواج الکترومغناطیس روی برخی فراسنجه‌های اسپرم قوچ و شاخص استرس اسید تیوبابیوتیریک (TBRS) به عنوان یک مدل حیوانی می‌باشد. نمونه‌های منی پس از جمع آوری به کمک واژن مصنوعی، در دستگاه تولید کننده امواج الکترومغناطیس با شدت های مختلف شامل کنترل، 1، 1/5، 2، 2/5، 3، و 3/5 میلی تسلا قرار داده شد. فراسنجه‌های منی با میکروسکوپ مجهز به کامپیوتر (کاسا) ارزیابی و زنده‌مانی اسپرم توسط کیت دیف کوئیک تعیین شد. بیش ترین تحرک در شدت 1 میلی تسلا و کم ترین آن در 2 میلی تسلا بود (85 درصد در مقابل 45/5 درصد). تحرک اسپرم در 3 میلی تسلا افزایش و به 74% رسید ولی پس از آن در شدت 3/5 به طور معنی‌داری کاهش و به 50/5 درصد رسید. زنده‌مانی در تیمار 3 و 3/5 میلی‌تسلا به ترتیب به 76/4 و 85/2 درصد رسید. زنده‌مانی در شدت 2/5 میلی‌تسلا نسبت به سایر تیمارها کاهش معنی‌داری داشت. غلظت TBARS به طوری معنی‌داری در شدت 3 میلی تسلا نسبت به گروه شاهد و سایر تیمارها پائین‌تر آمد. قرار دادن اسپرم در شدت‌های مختلف امواج اثرات متفاوتی را ایجاد می‌کند. می‌توان از برخی شدت‌ها در درمان ناباروری کمک گرفت.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of different intensities of electromagnetic field on some parameters and stress index in semen Lori Bakhtiari ram

نویسندگان [English]

  • Ruzita Bababasi
  • Saied Mohammadzadeh
  • Seyed Mojtaba Musavi
Department of Animal Science, Faculty of Agriculture, Lorestan University, Khoramabad, Iran
چکیده [English]

Electromagnetic fields and radio frequency radiation interact in human body tissues and can have effects on reproduction and the most mentioned side effects of the impact waves is in the enzyme reproductive system, male sex hormones, bone marrow, heart rate, hematological changes and neurobehavioral disorders.. This research is to investigate different electromagnetic intensity on some parameters of ram semen and TBARS stress index as an animal model. After semen collection with the help of an artificial vagina, samples were placed in a device that produces electromagnetic waves with different intensities, including control, 1, 1.5, 2, 2.5, 3, and 3.5 millitesla (mT). The Semen parameters were evaluated with a microscope equipped with a computer, Computer Assisted Sperm Analysis (CASA). The sperm viability was determined by Dif Quick kit. The highest motility was at the intensity of 1 mT and the lowest at 2 mT (85% vs 45.5%). The sperm motility increased at 3 mT and reached 74%, but then decreased significantly at 3.5 mT intensity and reached 50.5%. The Sperm viability in 3 and 3.5 mT reached 76.4 and 85.2% respectively. There was a significant decrease in viability at the intensity of 2.5 mT compared to other treatments. The concentration of TBARS significantly decreased at the intensity of 3 mT compared to the control group and other treatments. The dissimilar intensities create different effects. It is possible to get help some intensity in sterility treatment.

کلیدواژه‌ها [English]

  • Electromagnetic field
  • Motility
  • Semen
  • TBARS concentration
  • Viability

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فصلنامه محیط زیست جانوری
دوره 15، شماره 3
آبان 1402
صفحه 35-40

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