نوع مقاله : فیزیولوژی (جانوری)
نویسندگان
1 موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات،آموزش و ترویج کشاورزی، تهران، صندوق پستی: 141556116
2 گروه شیلات، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، صندوق پستی: 1477893855
3 گروه بهداشت آبزیان، دانشکده دامپزشکی، دانشگاه تهران، ایران، صندوق پستی: 1419963111
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Estrogenic effect of 4 - nonylphenol on plasma vitellogenin and sex steroid hormones (17 – β-estradiol ,testosterone and progesterone ) were studied in immature Koi carp . In order to, fish for
over three weeks Received Intraperitoneal injection of 10 , 50 and 100 microgram per gram of 4 -nonylphenol and 2 microgram per gram of 17- β-estradiol per body weight . Fish samples
were taken on days 0 and 22. The concentration of sex hormones in plasma measured by radioimmunoassay
and Plasma vitellogenin levels were measured by ELISAThe results showed that amount of vitellogenin
protein and estradiol hormne one at concentration 50 micrograms of 4- nonylphenol (51/66± 21/3)
and 2 micrograms of 17 β-estradiol (58±6/02) in males and females increased significantly compared with
the control group(P <0.05).But the changes in vitellogenin and 17- β-estradiol at a concentration of 100
micrograms nonylphenol showed significantly reduced compared with concentrations of 10 and 50
micrograms of nonylphenol (P <0.05). In the case of progesterone in all the groups that were treated with
nonylphenol ,the effect was not showed statistically significant,and only at concentrations of 2 micrograms
17-β-estradiol showed significant decrease(P> 0.05),Also testosterone levels,except in group 2 microgram
17-β-estradiol treated group had no significan difference(P >0.05). These results indicate harmful effects of 4-
nonylphenol using hormonal markers and assess the susceptibility of immature Koi Carp are against these
compounds .
کلیدواژه‌ها [English]
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