نوع مقاله : ژنتیک
نویسندگان
1 گروه علوم دامی، پردیس بین الملل دانشگاه فردوسی مشهد، صندوق پستی: 64955
2 گروه علوم دامی، دانشکده کشاورزی دانشگاه فردوسی مشهد، صندوق پستی: 91775
3 دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، صندوق پستی: 15739
4 سازمان تحقیقات، آموزش و ترویج کشاورزی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گلستان، صندوق پستی: 85799
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
This study aimed to study polymorphism of myostatin gene promoter and its association with growth and carcass traits in Mazandaran native chicken. For this purpose, 200 male chicks of Mazandaran native fowls reared in the same conditions and all were slaughtered at 12 weeks of age, were used. Traits before and after slaughter were measured and recorded. Before the destruction of all birds, blood sample and extraction of DNA from samples were performed by Cinnagen kit. Then, the desired locus of gene was amplified using specific primers and genotyped using PCR-RFLP method by specific enzyme. Phenotypic and genotypic data analysis conducted using statistical software of SAS9.2. The frequency of each allele (+) and (-) were estimated to be 0.77 and 0.23 in myostatin locus, respectively. Hardy-Weinberg equilibrium study using Chi-square test showed that the study population in loci is not in balance. The results showed a significant association between myostatin gene genotypes and traits of live weights at 12 weeks of age, carcass weight, heart weight and gizzard weight (P <0.05). Based on the results of this research can be concluded that myostatin gene can be used as a candidate for growth and carcass traits in Mazandaran native fowls breeding programs.
کلیدواژهها [English]