تاثیر هم زمان شدت و دوره نوری بر تکامل فعالیت آنزیم های گوارشی لارو تاس ماهی ایرانی (Acipenser persicus)

نوع مقاله : فیزیولوژی (جانوری)

نویسندگان

1 گروه بیولوژی و تکثیر و پرورش آبزیان، پژوهشکده آرتمیا و آبزی پروری، دانشگاه ارومیه. ارومیه، ایران. صندوق پستی: 57135165

2 مؤسسه تحقیقات بین‌المللی تاس‌ماهیان دریای خزر، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ایران. صندوق پستی: 416353464

چکیده

در تحقیق حاضر تاثیر دوره­ های نوری مختلف و شدت نور بر فعالیت آنزیم­ های گوارشی (پپسین، تریپسین، کموتریپسین، لیپاز، آمیلاز و آلکالین فسفاتاز) لارو تاس­ ماهی ایرانی (Acipenser persicus ) پس از تخم­ گشایی تا جذب کامل کیسه زرده مورد ارزیابی قرار گرفت. لاروهای تازه تفریخ شده تاس ­ماهی ایرانی (قره­برون) به ­ترتیب با میانگین وزنی و طولی 0/08±23/19میلی ­گرم و 0/03±35/11 میلی­ متر تحت شش تیمار با دوره­ های مختلف روشنایی و تاریکی L00D:24، D12L:12،D 00L:24 و در سه شدت نور 50، 150 و 300 لوکس به­ جز تیمار تاریکی کامل که فقط دارای یک شدت نور 10-0 لوکس بود، قرار گرفتند. در طول مدت پروژه لاروها در شرایط پرورشی یکسان (pH، دما، دبی آب و میزان تغذیه) قرار داشتند. نتایج حاصله نشان داد که فعالیت پپسین و تریپسین در روز نهم پس از تخم ­گشایی (dph) به ­ترتیب در تیمار 5 با شدت نور 150 لوکس و 12 ساعت روشنایی و تیمار 2 در 24 روشنایی و 150 لوکس بیش ­ترین میزان را دارا بود و به ­صورت معنی­ داری بالاتر از تیمار شاهد بود. فعالیت آنزیم آلکالین فسفاتاز در تیمار 2 و آمیلاز و کموتریپسین به ­ترتیب در دوره نوری L12 وL 24 و شدت نور 300 لوکس از فعالیت بیش ­تری برخوردار بود ولی با تیمار شاهد اختلاف معنی ­داری نداشتند. نتایج این تحقیق نشان داد نور فاکتور مهمی در مراحل اولیه تکامل سیستم گوارش ماهی قره­ برون به­ شمار می ­رود. هم ­چنین اغلب آنزیم ­های گوارشی تحت دوره نوری 12 ساعت و شدت نور بین 150 تا 300 لوکس فعالیت بیش ­تری از خود نشان دادند.

کلیدواژه‌ها

عنوان مقاله [English]

The impact of light intensity and photoperiod on ontogeny of digestive enzymes activity of Persian sturgeon (Acipenser persicus Borodin, 1897)

نویسندگان [English]

  • Farzaneh Noori 1
  • Rezvanolah Kazemi 2
  • Elaheh Hasan Nataj Niazi 1
1 Department of Biology and Aquaculture, Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran
2 Agriculture Research, Education and Extension Organization (AREEO), International Sturgeon Research Institute, Rasht, Iran
چکیده [English]

In the present study the impact of different photoperiod and light intensity on digestive enzymes activity (pepsin, trypsin, chymotrypsin, lipase, amylase and alkaline phosphatase) of Persian sturgeon (Acipenser persicus) from hatching until complete absorption of yolk sac were monitored. The newly hatched Persian sturgeon larvae with average weight and length of 19.23 ± 0.08 mg and 11.35 ± 0.03 mm respectively were treated with 3 photoperiods (24L, 12L, 24D) and 3 light intensities (50, 150 and 300 lux). The light intensity for 24D was 0-10 lux. The larvae in different treatments were cultured under similar experimental conditions (pH, temperature, water flow and feeding rate). The results demonstrated significantly higher activity of pepsin and trypsin at 9 days post hatch (dph) in treatment 2 (12L-150 lux light intensity) and in treatment 5 (24L-150 lux light intensity) compared to control respectively.  The alkaline phosphatase was higher in treatment 2 whereas amylase and chymotrypsin exhibited higher activity under 12L-300 lux, however showed no significant difference with control. In conclusion the results of this study indicated that light is an important physical factors in ontogeny of Persian sturgeon digestive system during early larval stages. Moreover most of the enzymes showed higher activity under 12L and 150 and 300 lux light intensity.

کلیدواژه‌ها [English]

  • Persian sturgeon larvae
  • Ontogeny
  • light intensity
  • Photoperiod
  • Digestive enzymes

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فصلنامه محیط زیست جانوری
دوره 10، شماره 1
فروردین 1397
صفحه 213-222

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