تاثیر هم زمان شدت و دوره نوری بر تکامل فعالیت آنزیم های گوارشی لارو تاس ماهی ایرانی (Acipenser persicus)

نوع مقاله: فیزیولوژی (جانوری)

نویسندگان

1 گروه بیولوژی و تکثیر و پرورش آبزیان، پژوهشکده آرتمیا و آبزی پروری، دانشگاه ارومیه. ارومیه، ایران. صندوق پستی: 57135165

2 مؤسسه تحقیقات بین‌المللی تاس‌ماهیان دریای خزر، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ایران. صندوق پستی: 416353464

چکیده

در تحقیق حاضر تاثیر دوره­ های نوری مختلف و شدت نور بر فعالیت آنزیم­ های گوارشی (پپسین، تریپسین، کموتریپسین، لیپاز، آمیلاز و آلکالین فسفاتاز) لارو تاس­ ماهی ایرانی (Acipenser persicus ) پس از تخم­ گشایی تا جذب کامل کیسه زرده مورد ارزیابی قرار گرفت. لاروهای تازه تفریخ شده تاس ­ماهی ایرانی (قره­برون) به ­ترتیب با میانگین وزنی و طولی 0/08±23/19میلی ­گرم و 0/03±35/11 میلی­ متر تحت شش تیمار با دوره­ های مختلف روشنایی و تاریکی L00D:24، D12L:12،D 00L:24 و در سه شدت نور 50، 150 و 300 لوکس به­ جز تیمار تاریکی کامل که فقط دارای یک شدت نور 10-0 لوکس بود، قرار گرفتند. در طول مدت پروژه لاروها در شرایط پرورشی یکسان (pH، دما، دبی آب و میزان تغذیه) قرار داشتند. نتایج حاصله نشان داد که فعالیت پپسین و تریپسین در روز نهم پس از تخم ­گشایی (dph) به ­ترتیب در تیمار 5 با شدت نور 150 لوکس و 12 ساعت روشنایی و تیمار 2 در 24 روشنایی و 150 لوکس بیش ­ترین میزان را دارا بود و به ­صورت معنی­ داری بالاتر از تیمار شاهد بود. فعالیت آنزیم آلکالین فسفاتاز در تیمار 2 و آمیلاز و کموتریپسین به ­ترتیب در دوره نوری L12 وL 24 و شدت نور 300 لوکس از فعالیت بیش ­تری برخوردار بود ولی با تیمار شاهد اختلاف معنی ­داری نداشتند. نتایج این تحقیق نشان داد نور فاکتور مهمی در مراحل اولیه تکامل سیستم گوارش ماهی قره­ برون به­ شمار می ­رود. هم ­چنین اغلب آنزیم ­های گوارشی تحت دوره نوری 12 ساعت و شدت نور بین 150 تا 300 لوکس فعالیت بیش ­تری از خود نشان دادند.

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