فعالیت آنتی اکسیدان های تام و اکسیدنیتریک در مایع سمینال موش های نر نژاد Balb/c تیمار شده با غلظت های مختلف نانوذرات نقره

نوع مقاله : زیست شناسی (جانوری)

نویسنده

گروه بیوشیمی، دانشگاه آزاد اسلامی، واحد ساری، ایران، کد پستی: 4816613485

چکیده

 با توجه به نقش نانوذرات نقره در القاء تولید رادیکال­ های آزاد، در این مطالعه اثرات سمی نانوذرات نقره بر پارامترهای اسپرم، غلظت نیتریک اکسید (NO) و فعالیت آنتی اکسیدان­ های تام در مایع سمینال موش نر مورد بررسی قرار گرفته است. در این مطالعه تجربی، 24 موش نر بالغ از نژاد سوری به ­صورت تصادفی به سه گروه تجربی و یک گروه شاهد تقسیم شدند. نانوذرات نقره با غلظت­ های 0/07 (A گروه)،  0/14 (گروه B) و 0/28 (گروه C) میکروگرم در هر روز، به ­ترتیب به گروه­ های اول تا سوم مورد مطالعه و به ­صورت دهانی به ­مدت پنج هفته داده شد. سپس فعالیت آنتی ­اکسیدان­ های تام و نیتریک اکسید به ­ترتیب به ­روش ­های FRAP(Ferric reducing antioxidant of power) و کیت NO اندازه­ گیری شد. پارامترهای اسپرمی به ­روش میکروسکوپی مورد آنالیز قرار گرفتند. کاهش معنی­ داری در میانگین کیفیت پارامترهای اسپرمی در گروه­ های تیمار شده با نانوذره، به­ خصوص گروه­ های B و C در مقایسه با گروه شاهد مشاهده گردید (0/01>p). تفاوت معنی­ داری در میانگین غلظت FRAP بین گروه ­های تیمارشده A(28/14±290/29) ،B(39/32±225/47) ،C(22/73±225/54 و  گروه شاهد (19/08±300/18) میکرومول بر لیتر مشاهده گردید (0/001>p). تفاوت معنی­ داری در میانگین NO نیز مشاهده گردید، به ­طوری ­که گروه C بیش ­ترین میانگین NO را در مقایسه با سایر گروه ­ها داشت. نانوذرات نقره با کاهش فعالیت آنتی ­اکسیدان­ های تام و افزایش NO سبب کاهش پارامترهای اسپرم می­ گردد که اثرات آن وابسته دوز نیز می­ باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Total antioxidant capacity and nitric oxide values in seminal plasma of Balb/c male rats treated with different concentrations of silver nanoparticle

نویسنده [English]

  • ISSA LAYALI
Department of Biochemistry, Islamic Azad University, Sari Branch, Sari, Iran
چکیده [English]

According to the harmful effects of silver nanoparticles induced by free radicals production, it is assumed that it can lead to reduced level of seminal total antioxidant capacity and then poor sperm quality. In this research, we aimed to investigate the toxic effects of silver nanoparticles on sperm parameters quality as well as total antioxidant capacity and nitric oxide (NO) in seminal plasma of male rats. In this experimental research, 24 male rats (Balb/c) were randomly divided into four groups including: controls (n=6), treatment group A (n=6), treatment group B (n=6) and treatment group C (n=6). Commercial silver nanoparticle was injected orally in a single dose (A=0.007μg, B=0.14μg and C=0.28μg per day) for 4 consecutive weeks. After that seminal total antioxidant capacity and NO values were measured using Ferric reducing antioxidant of power (FRAP) and microscopic techniques, respectively. Sperm parameters quality was considered by microscopic methods. There was a significant reduction in sperm parameters quality in treatment groups, especially in group C and group B, when compared with controls (p<0.01). There was a significant difference for FRAP between study groups of A (290.29±28.14), B (225.47±39.32), C (225.54±22.73) and control (300.18±19.08) group (p<0.001). There was a significant in mean of NO levels between groups (p<0.05), in which group C demonstrated higher mean value of NO concentration compared to the other groups. Silver nanoparticle leads to poor sperm quality via reducing of seminal total antioxidant capacity; however, its effect is dose dependent.         

کلیدواژه‌ها [English]

  • Silver nanoparticles
  • sperm parameters
  • Total Antioxidant Capacity
  • NO
  • male rats

مراجع

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فصلنامه محیط زیست جانوری
دوره 10، شماره 2
تیر 1397
صفحه 51-56

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