تأثیر اسید چرب امگا-3 و آنتی اکسیدان طی دوره فلاشینگ بر برخی فراسنجه های خون و خصوصیات بره زایی گوسفندان داشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم دامی، واحد اراک، دانشگاه آزاد اسلامی، اراک، ایران

چکیده

این مطالعه به‌منظور بررسی تأثیر استفاده از منابع اسید چرب امگا-3 و آنتی‌اکسیدان طی دوره‌ فلاشینگ بر برخی فراسنجه‌های خونی و خصوصیات بره‌زایی گوسفندان داشتی انجام شد. تجربیات اخیر نشان داده است که استفاده از امگا 3 و آنتی اکسیدان ها عملکرد تولید مثل را بهبود می بخشد. در این مطالعه، 256 رأس گوسفند، به‌طور تصادفی در 8 گروه آزمایشی و در هر گروه آزمایشی 32 رأس میش مورد مطالعه قرار گرفتند. میش‌ها، روزانه جیره‌های حاوی ویتامین E (800 واحد بین المللی/ روز)، روغن ماهی (35 گرم/کیلوگرم ماده خشک) و ال-کارنیتین (500 میلی گرم/کیلوگرم ماده خشک) و یا ترکیبی از این‌ها را دریافت کردند. نمونه‌های خون برای بررسی میزان لیپوپروتئین‌های با دانسیته‌ بالا، پایین، بسیار پایین، تری‌گلیسرید و کلسترول اخذ شد. تعداد فولیکول‌ها، دوقلوزایی، زنده‌مانی و سقط بررسی شد. غلظت سرمی لیپوپروتئین­ های با دانسیته پایین و لیپوپروتئین ­های با دانسیته بسیار پایین در تیمارهای روغن ماهی در مقایسه با دیگر تیمارها به­ طور معنی­ داری پایین ­تر بود (0/05>P).بیش ترین تعداد فولیکول در زمان قوچ اندازی، بیش ترین دوقلوزایی و کم ترین سقط در گروه‌های استفاده توام روغن ماهی و آنتی‌اکسیدان‌ها مشاهده شد (0/05>P). هم چنین کم ترین تعداد فولیکول‌ها، کم ترین دوقلوزایی و بیش ترین سقط در گروه روغن ماهی به تنهایی مشاهده شد (0/05>P). نتایج حاکی از این یافته‌ها نشان داد، ترکیبی از روغن ماهی و آنتی‌اکسیدان‌ها در جیره طی دوره‌ فلاشینگ، برای بهبود تولید مثل در میش‌ها در دوره‌ فلاشینگ توصیه می‌شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of omega-3 fatty acid and antioxidant sources during flushing period on some blood parameters and lambing performance of breeding ewes

نویسندگان [English]

  • Mahdi Hatami
  • Hossein Mansouri Yarahmadi
  • Jafar Fakhraei
  • Abbas Ahmadi
Department of Animal Science, Arak Branch, Islamic Azad University, Arak, Iran
چکیده [English]

This study was conducted to investigate the effect of using omega-3 fatty acid and antioxidant sources during flushing period on some blood parameters and lambing performance of breeding ewes. Recent experience has shown that the use of omega-3 and antioxidants improves reproduction performance. 256 ewes were randomly divided into 8 groups with 32 ewes. The ewes daily received diets containing vitamin E (800 IU/day), fish oil (35g/kg DM), and L-carnitine (500mg/kg DM) or a combination of these. The blood samples were collected to investigate HDL-C, LDL-C, VLDL-C, triglycerides and cholesterol. Number of follicles, twinning, viability and abortion were investigated. Adding fish oil into diet, decreased LDL-C and VLDL-C (P<0.05). The highest number of follicles at the time of lambing, the highest number of twinning and the lowest number of abortions were observed in the groups of combined use of fish oil and antioxidants (P<0.05). Also, the lowest number of follicles, the lowest number of twinning and the highest number of abortions were observed in the fish oil group alone (P<0.05). The results showed that a combination of fish oil and antioxidants in the diet during the flushing period is recommended to improve reproduction in ewes during the flushing period.

کلیدواژه‌ها [English]

  • Antioxidants
  • Bloods parameters
  • Fish oil
  • Flushing
  • Reproductive traits

مراجع

  1. Ali, A., Derar, D.R. and Alshahed, M., 2020. Management strategies, reproductive performance and causes of infertility in sheep flocks in the central region of Saudi Arabia. Tropical Animal Health and Production. 52(4): 1691-1697.
  2. Assan, N., 2020. Indicators of reproductive performance in goats and sheep meat production. Scientific Journal of Crop Science. 9(5): 608-619.
  3. Mugabe, L.C., Bagaldo, A.R., Barbosa, L.P., Araújo, F.L.D., Oliveira, B.Y.D.S., Silva, R.V.M.M., Oliveira, R.L. and Pinheiro, E.E.G., 2017. Biochemical and seminal parameters of lambs fed palm kernel cake under grazing system. Revista Brasileira de Zootecnia. 46: 670-677.
  4. Burritt B., McNeal, L., Miller, R. and Villar, F., 2012. The effect of flushing ewes on a commercial sheep operation on rangeland in Wyoming. Journal of the Nacca. 10: 1-14.
  5. Luan, X., Yan, Y., Zheng, Q., Wang, M., Chen, W., Yu, J. and Fang, J., 2020. Excessive reactive oxygen species induce apoptosis via the APPL1-Nrf2/HO-1 antioxidant signaling pathway in trophoblasts with missed abortion. Life Sciences. 254: 117781.
  6. Alemi, , Samadi, F., Dastar, B. and Hasani, S., 2018. Effect of different levels Artichoke and vitamin E on humoral immunity in Japanese quail. Journal of Animal Environmental. 9(4): 113-118.(In Persian)
  7. Dønnem, I., Randby, Å.T., Hektoen, L., Avdem, F., Meling, S., Våge, Å.Ø., Ådnøy, T., Steinheim, G. and Waage, S., 2015. Effect of vitamin E supplementation to ewes in late pregnancy on the rate of stillborn lambs. Small Ruminant Research. 125: 154-162.
  8. Liu, S., Masters, D., Ferguson, M. and Thompson, A., 2014. Vitamin E status and reproduction in sheep: potential implications for Australian sheep production. Animal Production Science. 54(6): 694-714.
  9. Nurlatifah, A., Khotijah, L., Komalasari, K. and Astuti, D.A., 2020. The effect of flushing with fatty acid supplementation in ewes ration on folliculogenesis. In IOP Conference Series: Earth and Environmental Science. 411(1): 012036.
  10. Abbasi, S., Fakhraei, J., Mansoori Yarahmadi, H. and Khaghani, Sh., 2020. Effects of different sources fat on performance immune system and intestinal microflora in commercial laying hens. Journal of Animal Environmental. 12(4): 225-230.(In Persian)
  11. Annett, R.W., Carson, A.F. and Dawson, L.E.R., 2008. Effects of digestible undegradable protein (DUP) supply and fish oil supplementation of ewes during late pregnancy on colostrum production and lamb output. Animal Feed Science and Technology. 146(3-4): 270-288.
  12. Agarwal, A., Sengupta, P. and Durairajanayagam, D., 2018. Role of L-carnitine in female infertility. Reproductive Biology and Endocrinology. 16(1): 1-18.
  13. El-Shahat, K.H., 2010. The effect of dietary supplementation with calcium salts of long chain fatty acids and/or l-carnitine on ovarian activity of Rahmani ewes. Animal reproduction science. 117(1-2): 78-82.
  14. Hernández-García, P.A., Mendoza-Martínez, G.D., Sánchez, N., Martínez-García, J.A., Plata-Pérez, F.X., Lara-Bueno, A. and Ferraro, S.M., 2017. Effects of increasing dietary concentrations of fish oil on lamb performance, ruminal fermentation, and leptin gene expression in perirenal fat. Revista Brasileira de Zootecnia. 46: 521-526.
  15. Liu, G., Ding, Y., Chen, Y. and Yang, Y., 2020. Effect of energy intake and L-carnitine on fattening performance, carcass traits, meat quality, blood metabolites, and gene expression of lamb. Small Ruminant Research. 183: 106025.
  16. National Research Council (NRC). 2007. Nutrient Requirements of Gots, 7th rev. ed. National Academy of Sciences, Washington, DC.
  17. Asbaghi, O., Choghakhori, R. and Abbasnezhad, A., 2019. Effect of Omega-3 and vitamin E co-supplementation on serum lipids concentrations in overweight patients with metabolic disorders: A systematic review and meta-analysis of randomized controlled trials. Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 13(4): 2525-2531.
  18. Ziaei, N., 2015. Effect of selenium and vitamin E supplementation on reproductive indices and biochemical metabolites in Raieni goats. Journal of Applied Animal Research. 43(4): 426-430.
  19. Cai, W.Y., Luo, X., Chen, E., Lv, H., Fu, K., Wu, X.K. and Xu, J., 2021. Serum Lipid Levels and Treatment Outcomes in Women Undergoing Assisted Reproduction: A Retrospective Cohort Study. Frontiers in endocrinology. 12: 633766.
  20. Leroy, J.L.M.R., Vanholder, T., Delanghe, J.R., Opsomer, G., Van Soom, A., Bols, P.E.J., Dewulf, J. and de Kruif, A., 2004. Metabolic changes in follicular fluid of the dominant follicle in high-yielding dairy cows early post-partum. Theriogenology. 62(6):1131-1143.
  21. Liu, M., Wang, D.Q.H., Black, D.D. and Tso, P., 2020. Differential Effect of Four-Week Feeding of Different Dietary Fats on the Accumulation of Fat and the Cholesterol and Triglyceride Contents in the Different Fat Depots. Nutrients. 12(11): 3241.
  22. Kim, S.Y., Chae, C.W., Lee, H.J., Jung, Y.H., Choi, G.E., Kim, J.S., Lim, J.R., Lee, J.E., Cho, J.H., Park, H. and Park, C., 2020. Sodium butyrate inhibits high cholesterol-induced neuronal amyloidogenesis by modulating NRF2 stabilization-mediated ROS levels: involvement of NOX2 and SOD1. Cell death & disease. 11(6): 1-19.
  23. Yulianto, A.B., Lokapirnasari, W.P., Najwan, R., Wardhani, H.C.P., Rahman, N.F.N., Huda, K. and Ulfah, N., 2020. Influence of Lactobacillus casei WB 315 and crude fish oil (CFO) on growth performance, EPA, DHA, HDL, LDL, cholesterol of meat broiler chickens. Iranian Journal of Microbiology. 12(2): 148-155.
  24. Quiroz, A., Molina, P., Santander, N., Gallardo, D., Rigotti, A. and Busso, D., 2020. Ovarian cholesterol efflux: ATP-binding cassette transporters and follicular fluid HDL regulate cholesterol content in mouse oocytes. Biology of reproduction. 102(2): 348-361.
  25. Ringseis, R., Keller, J. and Eder, K., 2018. Regulation of carnitine status in ruminants and efficacy of carnitine supplementation on performance and health aspects of ruminant livestock: a review. Archives of animal nutrition. 72(1): 1-30.
  26. Komal, F., Khan, M.K., Imran, M., Ahmad, M.H., Anwar, H., Ashfaq, U.A., Ahmad, N., Masroor, A., Ahmad, R.S., Nadeem, M. and Nisa, M.U., 2020. Impact of different omega-3 fatty acid sources on lipid, hormonal, blood glucose, weight gain and histopathological damages profile in PCOS rat model. Journal of Translational Medicine. 18(1): 1-11.
  27. Mossa, F., Jimenez-Krassel, F., Walsh, S., Berry, D.P., Butler, S.T., Folger, J., Smith, G.W., Ireland, J.L.H., Lonergan, P., Ireland, J.J. and Evans, A.C.O., 2010. Inherent capacity of the pituitary gland to produce gonadotropins is not influenced by the number of ovarian follicles≥ 3 mm in diameter in cattle. Reproduction, Fertility and Development. 22(3): 550-557.
  28. Rizzo, A., Roscino, M.T., Binetti, F. and Sciorsci, R.L., 2012. Roles of reactive oxygen species in female reproduction. Reproduction in Domestic Animals. 47(2): 344-352.
  29. Abu Ghazaleh, A.A. and Ishlak, A., 2014. Effects of incremental amounts of fish oil on trans fatty acids and b utyrivibrio bacteria in continuous culture fermenters. Journal of animal physiology and animal nutrition. 98(2): 271-278.
  30. Morrison, J.A., Glueck, C.J. and Wang, P., 2008. Dietary trans fatty acid intake is associated with increased fetal loss. Fertility and sterility. 90(2): 385-390.
  31. Bryk, D., Zapolska-Downar, D., Malecki, M., Hajdukiewicz, K. and Sitkiewicz, D., 2011. Trans fatty acids induce a proinflammatory response in endothelial cells through ROS-dependent nuclear factor-κB activation. Journal of Physiology and Pharmacology. 62(2): 229-238.
  32. Brigelius-Flohé, R., Banning, A., Kny, M. and Böl, G.F., 2004. Redox events in interleukin-1 signaling. Archives of biochemistry and biophysics. 423: 66-73.
  33. Chavarro, J.E., Rich-Edwards, J.W., Rosner, B.A. and Willett, W.C., 2007. Dietary fatty acid intakes and the risk of ovulatory infertility. The American journal of clinical nutrition. 85(1): 231-237.
  34. Agarwal, A., Gupta, S., and Sharma, R.K., 2005. Role of oxidative stress in female reproduction. Reproductive biology and endocrinology. 3(1): 1-21.
  35. Xie, H., Heier, C., Kien, B., Vesely, P.W., Tang, Z., Sexl, V., Schoiswohl, G., Strießnig-Bina, I., Hoefler, G., Zechner, R. and Schweiger, M., 2020. Adipose triglyceride lipase activity regulates cancer cell proliferation via AMP-kinase and mTOR signaling. Biochimica et Biophysica Acta (BBA). Molecular and Cell Biology of Lipids. 1865(9): 158737.
  36. Poljsak, B., Šuput, D. and Milisav, I., 2013. Achieving the balance between ROS and antioxidants: when to use the synthetic antioxidants. Oxidative medicine and cellular longevity. 2013: 956792.
  37. Agarwal, A., Aponte-Mellado, A., Premkumar, B.J., Shaman, A. and Gupta, S., 2012. The effects of oxidative stress on female reproduction: a review. Reproductive biology and endocrinology. 10(1): 1-31.
  38. Sanders, L.M., Henderson, C.E., Hong, M.Y., Barhoumi, R., Burghardt, R.C., Wang, N., Spinka, C.M., Carroll, R.J., Turner, N.D., Chapkin, R.S. and Lupton, J.R., 2004. An increase in reactive oxygen species by dietary fish oil coupled with the attenuation of antioxidant defenses by dietary pectin enhances rat colonocyte apoptosis. The Journal of nutrition. 134(12): 3233-3238.

 

فصلنامه محیط زیست جانوری
دوره 14، شماره 3
آبان 1401
صفحه 19-26

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