اثرات استفاده از واکسن غوطه وری گاروواک در پرورش بچه ماهی سی باس آسیایی (Lates calcarifer)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات و زیست شناسی دریا، پژوهشکده خلیج فارس، دانشگاه خلیج فارس، بوشهر، ایران

2 گروه زیست شناسی، دانشکده علوم زیستی، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی، پیشوا، ایران

10.22034/AEJ.2023.387492.2939

چکیده

هدف از این مطالعه بررسی اثرات واکسن غوطه وری گاروواک علیه بیماری های استرپتوکوکوزیس و لاکتوکوکوزیس در وزن های مختلف بر عملکرد رشد و تغذیه، برخی شاخص های ایمنی و میزان بازماندگی ماهی سی باس آسیایی (Lates calcarifer) بود. ماهیان با میانگین وزنی 5، 10 و 14 گرم در تانک های مدور 300 لیتری پلی اتیلنی در 6 تیمار و دو تکرار شامل تیمارهای واکسینه شده در وزن های پایین (5 گرم)، متوسط (10 گرم) و بالا (14 گرم) (ST, MT, LT) و گروه های شاهد هر یک از وزن ها بدون اعمال واکسیناسیون (SC, MC, LC) مورد بررسی قرار گرفتند. غذادهی دو بار در روز و تا حد سیری به مدت 8 هفته انجام شد. نتایج حاصل بیانگر این بود که واکسن غوطه وری گاروواک اثر معنی داری بر عملکرد رشد و تغذیه ماهیان واکسینه شده و شاهد ندارد (0/05<P). در مورد شاخص های ایمنی میزان پروتئین کل و آلبومین در تیمار واکسینه شده وزن پایین (ST) به صورت معنی داری بالاتر از گروه شاهد وزن پایین (SC) بود (0/05>P) و در تیمار وزن متوسط و وزن بالا تفاوت معنی داری بین گروه های واکسینه شده و شاهد مشاهده نگردید (0/05<P). هم چنین انجام واکسیناسیون تاثیر معنی داری بر میزان تیتر آنتی بادی و لیزوزیم در هیچ کدام از تیمارها نشان نداد (0/05<P). علاوه بر این، میزان بازماندگی نیز در پایان آزمایش اختلاف معنی داری بین تیمارهای واکسینه و گروه شاهد نشان نداد (0/05<P). با این حال، نتایج مربوط به چالش باکتریایی نشان داد که میزان تلفات در تیمار واکسینه شده وزن پایین (ST) به صورت معنی داری نسبت به گروه شاهد وزن متوسط (MC) کم تر بود (0/05>P). با توجه به نتایج کلی این مطالعه، تجویز واکسن غوطه وری گاروواک بر علیه بیماری استرپتوکوکوزیس توانسته است در وزن پایین ماهی سی باس آسیایی (5 گرم) میزان بازماندگی بعد از چالش باکتریایی را بهبود ببخشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effects of using GaroVak immersion vaccine in rearing juvenile Asian sea bass (Lates calcarifer)

نویسندگان [English]

  • Vahid Morshedi 1
  • Nehzat Bakhshi 1
  • Hadi Ebrahimi 1
  • Siamak Yousefi Siahkalroudi 2
1 Department of Fisheries, Persian Gulf Research Institute, Persian Gulf University, Bushehr, Iran
2 Department of Biology, Faculty of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Pishva, Iran
چکیده [English]

The aim of this study was to investigate the effects of GaroVak immersion vaccine against lactococcosis and streptococcosis disease in different weights on growth and feeding performance and some immunological responses and survival rate of Asian Sea bass (Lates calcarifer). Fish with an average weight of 5, 10 and 14 g in 300-liter polyethylene circular tanks in 6 treatments and 2 repetitions including vaccinated groups in low (5 g), medium (10 g) and high-weights (14 g) (ST, MT, LT) and control groups of each the weight without vaccination (SC, MC, LC) were examined. Feeding was done twice a day ad libitum for 8 weeks. The obtained results showed that GaroVak immersion vaccine has no significant effect on growth and feeding performance the vaccinated fish and the control fish (P>0.05). Regarding immunological responses, the amount of total protein and albumin was significantly higher in the low weight-treatment (ST) compared to the low-weight control group (SC), and no significant difference was observed in the medium-weight and the high-weight between the vaccinated treatments and the control group (P>0.05). Also, vaccination did not show a significant effect on antibody titer and lysozyme activity in any of the treatments (P>0.05). In addition, the survival rate at the end of the experiment did not show a significant difference between the vaccinated treatments and the control group (P> 0.05). However, the result of the bacterial challenge showed that mortality rate in the low-weight vaccinated group (ST) was lower than the medium-weight control group (MC) (P<0.05). According to the general results of this study, administration of GaroVak immersion vaccine against streptococcosis disease has been able to improve the survival rate after bacterial challenge in low-weight of Asian seabass (5 g).

کلیدواژه‌ها [English]

  • Immersion vaccine
  • Survival rate
  • Bacterial challenge
  • Growth and feeding performance
  • Asian sea bass

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فصلنامه محیط زیست جانوری
دوره 15، شماره 1
خرداد 1402
صفحه 191-200

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