عنوان مقاله [English]
The aim of this study was to evaluate the probiotic effects of Enterococcus faecium and Lactobacillus brevis on blood parameters of Sander lucioperca weighing 14±1 g. The number of fish was 225 completely randomly distributed in 5 treatments with three replications and 15 in each the ponds. Dietary treatments containing Enterococcus faecium and Lactobacillus brevis (1010, 108 probiotic cells per gram of diet) and probiotic-free control treatments were fed in three replications for 6 weeks and then evaluated for blood. Nutritional treatments were named, A2, A1,B1 ,B2, respectively. A1 (Enterococcus faecium probiotic (number of bacteria/g 1010 + basic food), A2 (Enterococcus faecium probiotic (number of bacteria/g 108+ basic food), B1 (Lactobacillus brevis probiotic) Number of bacteria/g 1010 + Basic food), B2 (probiotic Lactobacillus brevis) number of bacteria /g 1010 + basic food) and control treatment were Lactobacillus (basic food + physiological serum) Hematocrit (HCT) was measured using hematocrit capillary tubes and a hematocrit centrifuge. Hemoglobin by chlorometric method with a wavelength of 540 nm in a spectrophotometer was measured. The results showed that HCT increased in A1 treatment compared to control and A2 groups. There were no significant differences in treatments B1 and B2, but they had a significant increase compared to the control group(P<0.05) Leukocyte count in probiotic treatments was higher than the control group was significantly higher (P<0.05) . Lymphocyte counts in fish fed diets containing probiotics were significantly higher than in the control group. MCV had a significant difference in treatments B1 and A1 and control with A2 and B2 (P<0.05). Howevr, other parameters e.g. RBC, HB, MCH, MCHC had no significant different (P>0.05). The present study showed that the addition of probiotic bacteria in treatments B1, A1 to the diet of Sander lucioperca can have a favorable effect on their blood factors.