اثر آنتی‌اکسیدان‌های عصاره زعفران و زعفران تیمار شده با کیتوزان و ایزونیازید بر پارامترهای بیوشیمیایی آنزیم‌های کبدی در رت‌

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه بیوشیمی، دانشکده علوم، دانشگاه پیام نور، تهران، ایران

2 گروه شیمی آلی، دانشکده علوم، دانشگاه پیام نور، تهران، ایران

3 گروه علوم دامی، دانشکده کشاورزی، دانشگاه پیام نور، تهران، ایران

10.22034/aej.2021.314306.2683

چکیده

زعفران از 3000 سال پیش به‌عنوان چاشنی، رنگ، عطر و همچنین به‌عنوان گیاه دارویی استفاده شده است. هدف این تحقیق بررسی اثر زعفران بر قند پلاسما، چربی و آنزیم‌های کبدی است. در این مطالعه 60 سر موش سفید آزمایشگاهی نژاد ویستار نر بالغ با میانگین وزن 200 تا 250 گرم، به شش گروه شاهد، عصاره خوراکی زعفران، عصاره زعفران تیمار شده با کیتوزان، تیمار با ایزونیازید، تیمار با ایزونیازید (همراه با زعفران) و گروه ایزونیازید (زعفران تیمار شده با کیتوزان) تقسیم شدند. عصاره زعفران و تیمار کیتوزان برای القاء اثر اکسیداتیو ایزونیازید به‌صورت گاواژ استفاده شد. برای مقایسه اثرات آنتی‌اکسیدانی زعفران و زعفران تیمار شده با کیتوزان از روش DPPH استفاده گردید. استفاده از عصاره گیاه زعفران با کاهش تری گلیسیرید (31%)، کلسترول (35%)، LDL (22%) و ALP (16%) همراه بود و نیز باعث افزایش HDL (32%) سرم شد. هم چنین عصاره زعفران تیمار با کیتوزان باعث کاهش LDL (21%)، ALT (7%) و باعث افزایش HDL (26%) سرم گردید. در گروه‌های دریافت‌کننده ایزونیازید، گروه دریافت‌کننده ایزونیازید و زعفران، سطح سرمی آنزیم‌های ALT (افزایش %7 تا %16)، ALP (افزایش 26% تا 69%) و نیز کلسترول (افزایش %3 تا 31%)، تری گلیسیرید (افزایش 18% تا 44%) و گلوکز (افزایش 4% تا 14%) نسبت به گروه شاهد افزایش قابل‌توجهی نشان دادند. از این‌رو باتوجه‌به اثرات مثبت استفاده از زعفران و زعفران تیمار شده با کیتوزان، اثرات بهبوددهنده فاکتورهای ارزیابی‌شده قابل‌توجه است و باتوجه‌به تأثیرات زعفران بر قند خون و تری گلیسیرید و نیز آنزیم‌های کبدی می‌تواند در کنترل قند خون و چربی خون اثر مثبت داشته باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of saffron extract antioxidants and treated saffron with chitosan and isoniazid on biochemical parameters of liver enzymes in rats

نویسندگان [English]

  • Shahriar Saeidian 1
  • Bahaaldin Rashidzadeh 2
  • Zhilla Zarei 3
  • Mohammad Haidari 3
1 Department of Biochemistry, Faculty of Science, Payam Noor University, Tehran, Iran
2 Department of Organic Chemistry, Faculty of Science, Payam Noor University, Tehran, Iran
3 Department of Animal Science, Faculty of Agriculture, Payam Noor University, Tehran, Iran
چکیده [English]

Saffron for 3000 years ago was valued as culinary condiment, dye, perfume and medicinal herb. The aim of research was to examine the effects of saffron supplementation on glucose levels, lipids and liver enzymes. In present study, the homogenate of saffron and saffron treated with chitosan was extracted after drying using 96% ethanol. Then, ethanol solvent was removed using rotary equipment. 60 Wistar rats (200-250g) were divided into six groups, including normal group, saffron group, saffron group treated with Chitosan, isoniazid group, isoniazid-saffron group and isoniazid-saffron group treated with Chitosan. For induction of oxidative effect, oral extract of saffron and saffron treated with chitosan was used as gavage. The DPPH method was used to compare the antioxidant effects of chitosan extract. The use of extract of saffron led to decreasing of triglyceride(31%), cholesterol(35%), LDL(22%), ALP(16%) and increasing of HDL levels until to 32%. Chitosan-treated saffron extract also led to decreasinf of LDL to 21%, ALT to 7% and increasing of HDL to 26%. In group receiving isoniazid and saffron, serum levels of enzymes of ALT(7%-16%), ALP(26%-69%), cholesterol(3%-31%), triglycerides(18%-44%) and glucose(4%-14%) showed a significant increasing compared to the control group. Therefore, considering these positive effects of saffron and chitosan-treated saffron, it is obvious the enhancing effects of the evaluated factors. Regarding the effects of saffron on blood glucose and triglycerides and also Liver enzymes, they have a positive effect on blood glucose and lipid control. The results of this study confirmed the undesirable effects of isoniazide on the cells of body.

کلیدواژه‌ها [English]

  • Chitosan
  • Saffron
  • Serum biochemical parameters
  • Wistar rat
  • DPPH

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فصلنامه محیط زیست جانوری
دوره 15، شماره 1
خرداد 1402
صفحه 113-122

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