بررسی تأثیر عصاره نعناع (Mentha piperita L) و پونه (Mentha pulegium) بر شاخص های خونی و بیوشیمیایی در قزل‌آلای رنگین‌کمان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران

2 گروه دامپزشکی، دانشکده کشاورزی، واحد کرمانشاه، دانشگاه آزاد اسلامی، کرمانشاه، ایران

10.22034/aej.2022.354899.2862

چکیده

در سال‌های اخیر، به‌کارگیری مکمل‌های گیاهی به دلیل مزایای زیاد جایگزین مکمل‌های مصنوعی در آبزیان شده‌است. این پژوهش به منظور بررسی اثرات عصاره نعناع و پونه بر روی برخی از فراسنجه‌های خونی و بیوشیمیایی ماهی قزل‌آلای رنگین‌کمان انجام گردید. بدین منظور، تعداد 320 قطعه ماهی قزل‌آلا در قالب طرح کاملاً تصادفی با شش تیمار و چهار تکرار در استخر پرورش ماهی اطراف استان کرمانشاه ارزیابی شد. گروه‌های آزمایشی شامل (1): گروه شاهد (جیره فاقد عصاره پونه و نعناع)، (2): تیمار حاوی 1 درصد پونه، (3): تیمار حاوی 2 درصد پونه، (4): تیمار حاوی 1 درصد نعناع، (5) تیمار حاوی 2 درصد نعناع و (6) تیمار ترکیبی (1 درصد از هر عصاره) بودند. برخی از فراسنجه‌های خونی شامل تعداد گلبول‌های سفید و قرمز، هموگلوبین و فراسنجه‌های بیوشیمیایی شامل آلبومین، گلوبولین، گلوکز، تری‌گلیسیرید و آنزیم‌های آلکالین فسفاتاز، آلانین آمینو ترنسفراز و آسپارتات آمینوترانسفراز بررسی شد. نتایج نشان دادن که افزودن ترکیب دو عصاره پونه و نعناع به جیره، سبب افزایش معنی‌دار گلبول‌های سفید، گلبول‌های قرمز و هموگلوبین در مقایسه با افزودن هر یک از این عصاره‌ها به تنهایی گردید. عصاره 1 درصد نعناع بیش ترین تأثیر را بر تعداد گلوبولین خون داشت. بیشترین تأثیر معنی‌دار بر روی میزان آلبومین، مربوط به عصاره 2 درصد نعناع بود. بیش ترین میانگین گلوکز خون و آلبومین به تیمار حاوی 2 درصد نعناع و بیش ترین و کم ترین میزان تری‌گلیسیرید به ترتیب به عصاره 1 درصد نعناع و ترکیب دو عصاره نعناع و پونه تعلق گرفت. سطوح بالاتر هر دو عصاره پونه و نعناع موجب افزایش مقادیر آنزیم های آلکالین فسفاتاز، آلانین آمینو ترنسفراز و آسپارتات آمینوترانسفراز گردید. با توجه به نتایج مطالعه، به نظر می رسد در شرایط فیزیولوژیک، استفاده از عصاره پونه و نعناع ضمن بهبود عملکرد سیستم ایمنی و برخی فراسنجه‌های بیوشیمیایی خون، می تواند فیزیولوژی ماهی را بهبود بخشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of the effect of Mentha piperata and Mentha Pulegium extract on blood and biochemical parameters in Oncorhynchus mykiss

نویسندگان [English]

  • Maryam Karimi Dehkordi 1
  • Forogh Mohammadi 2
1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 Department of veterinary, Agriculture Faculty, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
چکیده [English]

In recent years, the application of herbal compounds has replaced artificial supplements in the fisheries due to their benefits. This study was performed to investigate the effects of Mentha piperata and Mentha Pulegium extracts on some blood and biochemical parameters in Oncorhynchus mykiss. For this purpose, 320 fish were evaluated in a completely randomized design with six treatments and four replications in a farm around Kermanshah Province. Experimental groups included (1): control group (basic diet), (2): treatment containing 1% Pennyrile, (3): treatment containing 2% M. Pulegium, (4): treatment containing 1% M. piperata, (5) treatments contained 2% M. piperata and (6) combination treatments (1% of each extract). Blood parameters including white and red blood cells, globulin, hemoglobin, and biochemical parameters, including albumin, glucose, triglycerides, and the enzymes alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase were evaluated. According to the results, the combination of M. pulegium and M. piperata extracts resulted in more white blood cells, red blood cells, and hemoglobin compared to the individual levels of both mint extracts. 1% M. piperata extract had the greatest effect on blood globulin count. The most significant effect on albumin content was observed in 2% M. piperata extract group. The highest mean blood glucose and albumin were found in the fish received 2 percent of M. piperata. Also, the highest levels of and the lowest triglyceride were observed in the fish supplemented with M. piperata extract and the combination of M. piperata and M. pulegium extracts, respectively. Higher levels of both M. pulegium and M. piperita L extracts increased the levels of alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase enzymes. According to the results of the study, it seems that in physiological conditions, the use of M. pulegium and M. piperata extracts can improve the physiology of fish while improving the function of the immune system and some blood biochemical parameters.

کلیدواژه‌ها [English]

  • M. Piperata
  • M.Pulegium
  • Blood parameters
  • Biochemical parameters
  • Oncorhynchus mykiss
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