بررسی اثر نسبت های مختلف کربن- نیتروژن در سیستم پرورش متراکم بایوفلاک بر شاخص های رشد و سلامت ماهی کپور معمولی (Cyprinus carpio)

نوع مقاله : سایر

نویسندگان

1 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز

2 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز

چکیده

فن‌آوری بایوفلاک سیستمی است که با تنظیم نسبت کربن و نیتروژن درون آب و افزایش تراکم میکروارگانیزم‌های هتروتروف برای پیشبرد منافع پرورش‌دهنده استفاده می‌شود. در این تحقیق اثر نسبت‌های مختلف کربن به ازت در تکنولوژی پرورش متراکم بایوفلاک بر شاخص‌های رشد و برخی شاخص‌های آسایش ماهی کپور معمولی ارزیابی گردید. به این منظور تعداد  420  عدد بچه ماهی کپور معمولی با وزن متوسط (5/9±40)گرم به چهار تیمار در سه تکرار تقسیم گردیدند: تیمار اول، دوم و سوم به­ترتیب به سیستم بایوفلاک با نسبت­های کربن به ازت  1:15، 1:20 و 1:25 اختصاص  داده شد، و تیمار چهارم تیمار شاهد بود. ماهی‌ها به ­مدت 90 روز پرورش داده شدند و به فواصل یک‌ماهه زیست‌سنجی و نمونه‌گیری انجام گرفت. شاخص‌های رشد و  برخی شاخص‌های سلامت ماهی شامل: خصوصیات ظاهری شامل رنگ ماهی، آلودگی انگلی و قارچی، بدشکلی ­ها، خصوصیات رفتاری (تنفسی، اشتها و نوع شنا) و ساختارهای بافتی آبشش، کبد، کلیه و پوست پرداخته شد. نتایج نشان دادند که  شاخص‌های رشد در تیمارهای بایوفلاک به ­طور معنی‌داری بهتر از تیمار شاهد بود ( 0/05>p). هرچند که شاخص‌های رشد مورد بررسی در بین سه  تیمارهای بایوفلاک تفاوت معنی‌داری نداشتند.  در بین شاخص‌های سلامت ماهی مورد بررسی نیزتغییرات قابل ملاحظه‌ای در بررسی‌های فوق مشاهده نشد و ماهیان در این شرایط توانستند با سلامت نسبی و قابل قبولی پرورش یابند و هیچ‌گونه ضایعه بافتی و تک‌یاخته‌ای بیماری‌زایی در مطالعات میکروسکوپی مشاهده نشد. به ­عنوان نتیجه‌گیری کلی می ­توان سیستم پرورش متراکم با فن‌آوری بایوفلاک را برای برخی گونه ­ها ازجمله کپور معمولی مناسب دانست.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of different C/N ratios effect at intensive biofloc system on growth performance and health of Common Carp (Cyprinus carpio)

نویسندگان [English]

  • Mohammad MEHDI HAGHPARAST RADMARD 1
  • Mojtaba Alishahi 1
  • Masoud Ghorbanpour 2
  • Ali shahriari 1
1 Department of Clinical sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz
2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz
چکیده [English]

Biofloc technology system that be done by adjusting the ratio of C/N in the water and increases the density of heterotrophic microorganisms are used to improve the farmer interests. At this study the effects of different ratios of carbon to nitrogen in intensive culture of common carp on growth and health  performance  was evaluated . for this purpose, a total of 420 healthy common carp with an average weight of 40 ±5.9 (SDِ±Mean) Were divided into four treatments with three replications include A(15:1), B(20:1),C(25:1) and D(control) . The fish were reared for 90 days . Biometery and sampling was carried at one- month interval. Growth performance and fish health indicators, including  Appearance, including color fish, Parasitic and fungal infections, deformities and behavioral characteristics (Respiratory, appetite and swimming) and gill structures, liver, kidney and skin were evaluated. The results showed that the index for the treatment of  Biofloc was significantly better than the control treatment(P<0.05). Although an exact growth parameters evaluated showed no significant difference among the three treatments of  biofloc. Among the health of the fish has been studied, significant changes were not observed in these studies and fish in these condition could be raised with relative health  and any tissue lesions and protozoan pathogen was not found in microscopic studies

کلیدواژه‌ها [English]

  • Biofloc
  • Common carp
  • Intensive system
  • histology
  • Fish health

مراجع

  1. Algers, B.; Blokhuis, H.J. and Broom, D.M., 2008. Animal welfare aspects of husbandry systems for farmed fish Carp. EFSA journal. Vol. 843, pp: 1-28.
  2. Algers, B.; Blokhuis, H.J.; Bøtner, A.; Broom, D.M.; Costa, P.; Domingo, M.; Greiner, M.; Hartung, J.; Koenen, F.; Müller-Graf, C. and Morton, D.B., 2009. Scientific opinion of the panel on animal health and welfare on a request from European Commission on general approach to fish welfare and the concept of sentience in fish. The European Food and Safety Authority Journal. Vol. 954, pp: .1-12.
  3. Azim, M.E.; Little, D.C. and Bron, J.E., 2008. Microbial protein production in activated suspension tanks manipulating C: N ratio in feed and the implications for fish culture. Bioresource Technology. Vol. 99, pp: 3590-3599. 
  4. Avnimelech, Y., 1999. Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture. Vol. 176, pp: 227-235.
  5. Anand, P.S.; Kohli, M.P.S.; Kumar, S.; Sundaray, J.K.; Roy, S.D.; Venkateshwarlu, G.; Sinha, A. and Pailan, G.H., 2014. Effect of dietary supplementation of biofloc on growth performance and digestive enzyme activities in Penaeus monodon. Aquaculture. Vol. 418, pp:108-115.
  6. APHA. 1998. Standard Methods for the Examination of the Water and Wastewater, 22nd edn. American Public Health Association, Washington, DC.
  7. Avnimelech, Y., 2007. Feeding with microbial flocs by tilapia in minimal discharge bioflocs technology ponds. Aquaculture. Vol. 264, pp: 140-147. 
  8. Bagni, M.; Civitareale, C.; Priori, A.; Ballerini, A.; Finoia, M.; Brambilla, G. and Marino, G., 2007. Preslaughter crowding stress and killing procedures affecting quality and welfare in sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata). Aquaculture. Vol. 263, pp: 52-60.
  9. Bucke, D.A.V.I.D., 1972. Some histological techniques applicable to fish tissues. In Disease of Fish. Proceeding of Symposium. Vol. 30, pp: 257-301.
  10. Caballero, M.J.; Izquierdo, M.S.; Kjørsvik, E.; Montero, D.; Socorro, J.; Fernández, A.J. and Rosenlund, G., 2003. Morphological aspects of intestinal cells from gilthead seabream (Sparus aurata) fed diets containing different lipid sources. Aquaculture. Vol. 225, pp: 325-340.
  11. Craig, S. and Helfrich, L.A., 2002. Understanding Fish Nutrition, Feeds and Feeding (Publication 420–256). Virginia Cooperative Extension, Yorktown (Virginia). 4 p.
  12. Chavan, V.R. and Muley, D.V., 2014. Effect of heavy metals on liver and gill of fish Cirrhinus mrigala. Int. J. Curr. Microbiol. App. Sci. Vol. 3, No. 5, pp: 277-288.
  13. Cengiz, E.I., 2006. Gill and kidney histopathology in the freshwater fish Cyprinus carpio after acute exposure to deltamethrin. Environmental Toxicology and Pharmacology. Vol. 22, No. 2, pp: 200-204.
  14. De Silva, S.S. and Anderson, T., 1995. Fish Nutrition in aquaculture. London, UK: Chapman and Hall.
  15. Dutta, H.M.; Adhikari, S.; Singh, N.K.; Roy, P.K. and Munshi, J.S.D., 1993. Histopathological changes induced by malathion in the liver of a freshwater catfish, Heteropneustes fossilis (Bloch). Bulletin of environmental contamination and toxicology. Vol. 51, No. 6, pp: 895-900.
  16. Erickson, R.J., 1985. An evaluation of mathematical models for the effects of pH and temperature on ammonia toxicity to aquatic organisms. Water Research. Vol. 19, No. 8, pp: 1047-1058.
  17. Emerenciano, M.; Ballester, E.L.C.; Cavalli, R.O. and Wasielesky, W., 2013. Effect of biofloc technology (BFT) on the early postlarval stage of pink shrimp Farfantepenaeus paulensis: growth performance, floc composition and salinity stress tolerance. Aquac Int. Vol. 19, pp: 891-901.
  18. Hargreaves, J.A., 2006. Photosynthetic suspended-growth systems in aquaculture. Aquacultural Engineering. Vol. 34, pp: 344-363.
  19. Hargreaves, J.A., 2013. Biofloc Production System for Aquaculture. Southern RegionalAquaculture Center (SRAC) Publication No. 4503.
  20. Handy, R.D. and Depledge, M.H., 1999. Physiological responses: their measurements and use as environmental biomarkers in ecotoxicology. Ecotoxicology. Vol. 8, pp: 329-349.
  21. Heidarieh, M.; Mirvaghefi, A.R.; Akbari, M.; Farahmand, H.; Sheikhzadeh, N.; Shahbazfar, A.A. and Behgar, M., 2012. Effect of dietary Ergosan on growth performance, digestive enzymes, intestinal histology, hematological parameters and body composition of rainbow trout (Oncorhynchus mykiss). Fish Physiol. Biochem.
    Doi 10.1007/s10695-012-9602-8.
  22. Hari, B.; Kurup, B.M.; Varghese, J.T.; Schrama, J.W. and Verdegem, M.C.J., 2006. The effect of carbohydrate addition on water quality and the nitrogen budget in extensive shrimp culture systems. Aquaculture. Vol. 252, No. 2, pp: 248-263.
  23. Ju, Z.Y.; Forster, I.; Conquest, L.; Dominy, W.; Kuo, W.C. and David Horgen, F., 2008. Determination of microbial community structures of shrimp floc cultures by biomarkers and analysis of floc amino acid profiles. Aquaculture Research. Vol. 39, No. 2, pp: 118-133.
  24. Kristiansen, T.S.; Ferno, A.; Holm, J.C.; Trivitera, L.;Bakke, S. and Fosseidengen, J.E., 2004. Swimming behaviour as an indicator of low growth rate and impaired welfare in Atlantic halibut (Hippoglossushippoglossus L.) reared at three stocking densities. Aquaculture. Vol. 230, pp: 137-151.
  25. Kramer, D.L., 1987. Dissolved oxygen and fish behaviour. Environ. Biol. Fish. Vol. 18, pp: 81-92.
  26. Kuhn, D.D.; Lawrence, A.L.; Boardman, G.D.; Patnaik, S.; Marsh, L. and Flick, G.J., 2010. Evaluation of two types of bioflocs derived from biological treatment of fish effluent as feed ingredients for Pacific white shrimp, Litopenaeus vannamei. Aquaculture. Vol. 303, No. 1, pp: 28-33.
  27. Luo, G.; Gao, Q.; Wang, C.; Liu, W.; Sun, D.; Li, L. and Tan, H., 2014. Growth, digestive activity, welfare, and partial cost-effectiveness of genetically improved farmed tilapia (Oreochromis niloticus) cultured in a recirculating aquaculture system and an indoor biofloc system. Aquaculture. Vol. 422, pp: 1-7.
  28. MacIntyre, C.M., 2008. Water quality and welfare assessment on United Kingdom trout farms. Chapter 2. Influences of water quality on the welfare of farmed rainbow trout. PhD thesis. Institute of Aquaculture, University of Stirling, UK.
  29. Morton, D.B., 1990. Guidelines on the recognition of pain, distress and discomfort in experimentalanimals. Europ. J. Pharmacol. Vol. 183, pp: 1583-1590.
  30. Mettam, J.J.; Oulton, L.J.; McCrohan, C.R. and Sneddon, L.U., 2011. The efficacy of three types of analgesic drugs in reducing pain in the rainbow trout, Oncorhynchus mykiss. Applied Animal Behaviour Science. Vol. 133, No. 3, pp: 265-274.
  31. Morton, A.B., 1990. A quantitative comparison of the behaviour of resident and transient forms of the killer whale off the central British Columbia coast.Reports of the International Whaling Commission Special. Vol. 12,
    pp: 245-248.
  32. McKim, J.M. and Erickson, R.J., 1991. Environmental impacts on the physiological mechanisms controlling xenobiotic transfer across fish gills. Physiological zoology. Vol. 64, No. 1, pp: 39-67.
  33. Mohamed, M.E., 2010. The effect of microbial biofloc on water quality, survival and growth of the green tiger shrimp (Penaeus semisulcatus) fed with different crude protein levels. Journal of the Arabian Aquaculture Society. Vol. 5, No. 2, pp: 119-142
  34. Noble, C.; Jones, H.A.C.; Damsgård, B.; Flood, M.J.; Midling, K.Ø.; Roque, A.; Sæther, B.S. and Cottee,
    S.Y., 2012.     Injuries and deformities in fish: their potential impacts upon aquacultural production and welfare. Fish physiology and biochemistry. Vol. 38, No. 1, pp: 61-83.
  35. Najdgerami, E.H.; Bakhshi, F. and Lakani, F.B., 2015. Effects of biofloc on growth performance, digestive enzyme activities and liver histology of common carp (Cyprinus carpio L.) fingerlings in zero-water exchange system. Fish physiol biochem. Vol. 10695, No. 15, pp: 151-159.
  36. Ostaszewska, T.; Dabrowski, K.; Czuminska, K.; Olech, W. and Olejniczak, M., 2005. Rearing of pikeperch larvae using formulated diets-first success with starter feeds. Aquaculture Research. Vol. 36, pp: 1167-1176.
  37. Ottesen, O.H. and Olafsen, J.A., 2000. Effects on survival and mucous cell proliferation of Atlantic halibut, Hippoglossus hippoglossus L., larvae following microflora manipulation. Aquaculture. Vol. 187, pp: 225-228.
    Doi: 10.1016/S0044-8486(00)00314-8.
  38. Odegard, J.; Olesen, I.; Dixon, P.; Jeney, Z.; Nielsen, H.M.; Way, K.; Joiner, C.; Jeney, G.; Ardó, L.; Rónyai, A. and Gjerde, B., 2010. Genetic analysis of common carp (Cyprinus carpio) strains. II: Resistance to koi herpesvirus and Aeromonas hydrophila and their relationship with pond survival. Aquaculture. Vol. 304, No. 1, pp: 7-13.
  39. Patriche, T.; Patriche, N.; Bocioc, E. and Coada, M.T., 2011. Serum biochemical parameters of farmed carp (Cyprinus carpio). Aquaculture, Aquarium, Conservation & Legislation-International Journal of the Bioflux Society (AACL Bioflux). Vol. 4, No. 2.
  40. Pan, L.Q. and Xu, W.J., 2012. Effects of bioflocs on growth performance, digestive enzyme activity and body composition of juvenile Litopenaeus vannamei in zero-water exchange tanks manipulating C/N ratio in feed. Aquaculture. Vol. 356, pp: 147-152.
  41. Poleksić, V.; Karan, V.; Elezović, I. and Nešković, N., 1995. Estimation of harmful effects of pesticides on fish: biochemical and histopathological approach. I Regional Symposium: Chemistry and the Environment, Vrnjačka Banja, Serbian Chemical Society, Proceedings. Vol. 1, pp: 487-490.
  42. Poleksić, V.; Rašković, B.; Marković, Z.; Dulić, Z.; Stanković, M.; Živić, I. and Lakić, N., 2007. Effects of different dietary protein sources on intestine and liver morphology of carp yearlings. Proceedings of the 3rd Serbian Congress for Microscopy. Belgrade, Serbia, Serbian Microscopy Society. pp: 237-238.
  43. Ruyet, J.P.; Labbe, L.; Bayon, N.L.; Severe, A. and Roux, A.L., 2008. Combined effects of water quality and stocking density on welfare and growth of rainbow trout (Oncorhynchus mykiss). Aquat. Vol. 21, pp: 185-195.
  44. Rašković B.; Poleksić V.; Živić I. and Spasić, M., 2010. Histology of carp (Cyprinus carpio, L.) gills and pond water quality in semiintensive production, Bulg. J. Agric. Sci. Vol. 16, pp: 253-262.
  45. Raskovic, B.; Jaric, I.; Koko, V.; Spasic, M.; Dulic, Z. and Markovic, Z., 2013. Histopathological indicators: a useful fish health monitoring tool in common carp (Cyprinus carpio Linnaeus, 1758) culture. J.Biol. Vol. 8, pp: 975-985.
  46. Relić, R.; Ćirić, M.; Radenković-Damnjanović, B. and Vučinić, M., 2009. Kvalitet vode i dobrobit riba. Zbornik radova XX Savetovanja “Dezinfekcija, dezinsekcija i deratizacija u zaštiti zdravlja životinja i ljudi” sa međunarodnim učešćem, Divčibare. pp: 199-206.
  47. Relic, R.; Hristov, S.; Poleksic, V. and Markovic, Z., 2009. Establishment of fish stress studies within the Center for fishery and applied hydrobiology at the Faculty of agriculture University of Belgrade. In book of abstracts of the Conference Aquaculture Europe. pp: 269-270.
  48. Rankin, J.C. and Bolis, L., 1984. 6 Hormonal Control of Water Movement Across the Gills. Fish physiology. Vol. 10, pp: 177-201.
  49. Rodrigues, E.D.L. and Fanta, E., 1998. Liver histopathology of the fish Brachydanio rerio Hamilton Buchman after acute exposure to sublethal levels of the organophosphate Dimethoate 500. Revista Brasileira de Zoologia. Vol. 15, No. 2, pp: 441-450.
  50. Segner, H. and Juario, J.V., 1986. Histological observations on the rearing of milkfish, Chanos chanos, fry using different diets. Journal of Applied Ichthyology. Vol. 2, pp: 162-172.
  51. Roberts, R.J., 1989. Fish pathology. Baillière Tindall, London.
  52. Svobodova, Z.; Lloyd, R.; Machova, J. and Vykusova, B., 1993. Water quality and fish health. EIFAC Technical Paper. No. 54. Rome, FAO. 59 p.
  53. Sutor, H.C. and Huntingford, F.A., 2002. Eye colour in juvenile Atlantic salmon: effects of social status, aggression and foraging success. J. Fish Biol. Vol. 61, pp: 606-614.
  54. Strüssmann, C.A. and Takashima, F., 1990. Hepatocyte nuclear size and nutritional condition of larval pejerrey, Odontesthes bonariensis (Cuvier et Valenciennes). Journal of Fish Biology. Vol. 36, pp: 59-65.
  55. Sharma, A.; Sharma, K. and Sangotra, R., 2015. Biofloc culture and its utilisation as feed in limited water exchange system for the culture of labeo rohita. Journal of international academic research for multidisciplinary. Vol. 2320, Vol. 5083. pp: 1-9.
  56. Souza, D.M.; Martins, Á.C.; Jensen, L., Wasielesky Jr, W., Monserrat, J.M. and Garcia, L.D.O., 2014. Effect of temperature on antioxidant enzymatic activity in the Pacific white shrimp Litopenaeus vannamei in a BFT (Biofloc technology) system. Marine and Freshwater Behaviour and Physiology. Vol. 47, No. 1, pp: 1-10.
  57. Svobodova, Z.; Blahova, J.; Modra, H. and Velíšek, J., 2009. The effect of transport on biochemical and haematological indices of common carp (Cyprinus carpio L.). Czech J Anim Sci. Vol. 54, No. 11, pp: 510-518.
  58. Turnbull, J.F.; Bell, A.; Adams, C.E.; Bron, J. and Huntingford, F.A., 2005. Stocking density and welfare of cage farmed Atlantic salmon: application of a multivariate analysis. Aquaculture. Vol. 243, pp: 121-132.
  59. Takashima, F. and Hibiya, T., 1982. An atlas of fish histology: normal and pathological features. Kodansha, distributed by Fischer, G., Tokyo.
  60. Tacon, A.G.J., 1992. Nutritional fish pathology: morphological signs of nutrient deficiency and toxicity in farmed fish. Food and Agriculture Organization of the United Nations, Rome.
  61. Vis, H.; Oehlenschlager, J.; Kuhlmann,H.; Munkner, W.; Robb, D.H.F. and Schelvis-Smit,A.A.M., 2001. Effect of the Commercial and Experimental Slaughter of Eels (Anguilla Anguilla L.) on Quality and Welfare. In: S.C. Kestin and P.D. Warriss (eds.) Farmed Fish Quality. Fishing News Books, Oxford, UK. pp: 234-248.
  62. Verreth, J.; Coppoolse, J. and Segner, H., 1994. The effect of low HUFA-and high HUFA-enriched Artemia, fed at different feeding levels, on growth, survival, tissue fatty acids and liver histology of Clarias gariepinus larvae. Aquaculture. Vol. 126, pp: 137-150.
  63. Widanari, A.; Ekasari, J. And Maryam, S., 2012. Evaluation of Biofloc Technology Application on Water Quality and Production Performance of Red Tilapia Oreochromis sp Cultured at Different Stocking Densities. Journal of Biosciences. Vol. 2, pp: 73-80.
  64. Xueqin, J.; Kania, P.W. and Buchmann, K., 2012. Comparative effects of four feed types on white spot disease susceptibility and skin immune parameters in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Fish Dis. Vol. 35, pp: 127-135. doi: 10.1111/j.1365-2761.2011.01329.
  65. Yuan, D.; Yi, Y.; Yakupitiyage, A.; Fitzimmons, K. and Diana, J.S., 2010. Effects of addition of red tilapia (Oreochromis spp.) at different densities and sizes on production, water quality and nutrient recovery of intensive culture of white shrimp (Litopenaeus vannamei) in cement tanks. Aquaculture. Vol. 298, No. 3, pp: 226-238.
  66. Zhao, P.; Huang, J.; Wang, X.H.; Song, X.L.; Yang, C.H.; Zhang, X.G. and Wang, G.C., 2012. The application of bioflocs technology in high-intensive, zero exchange farming systems of Marsupenaeus japonicus. Aquaculture. Vol. 354, pp: 97-106.
فصلنامه محیط زیست جانوری
دوره 10، شماره 2
تیر 1397
صفحه 169-170

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